RAMAN FLOW CYTOMETRY FOR DRUG DISCOVERY AND DIAGNOSTICS

用于药物发现和诊断的拉曼流式细胞术

• 批准号: 8361750
• 负责人: JOHN P NOLAN
• 金额: $ 4.47万
• 依托单位: LOS ALAMOS NAT SECTY-LOS ALAMOS NAT LAB
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-04-01 至 2013-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8361750
• 关键词: Academia; Area; Biomedical Engineering; Biomedical Research; Detection; Diagnostic; Engineering; Flow Cytometry; Funding; Government; Grant; Individual; Industry; Measurement; Molecular; Molecular Analysis; National Center for Research Resources; Optics; Plant Resins; Polymers; Principal Investigator; Property; Raman Spectrum Analysis; Reporting; Research; Research Infrastructure; Resources; Screening procedure; Sorting - Cell Movement; Source; Technology; Toxin; United States National Institutes of Health; base; biological systems; chemical synthesis; cost; drug discovery; high throughput screening; instrumentation; molecular assembly/self assembly; nanostructured; new technology; particle; pathogen; research and development; therapeutic development

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. The ability to make quantitative, high throughput molecular measurements of biological systems is a critical need for many areas of biomedical research. This Bioengineering Research Partnership (BRP) aims to develop a powerful new analytical platform for high throughput screening and selection based on Raman Flow Cytometry. This Partnership will develop new analytical instrumentation, optically encoded polymer resins for chemical synthesis and screening, and nanostructured materials with unique optically properties for sensitive reporting and encoding. The new technology will perform Raman spectroscopy on single particles in flow to enable new applications in sensitive multiplexed detection, drug discovery, and diagnostics. The Raman Flow Cytometry instrumentation and applications will be developed by a Partnership involving engineers, biologists, and chemists from academia, government and industry. In the first year of the Partnership, we will modify a commercial particle sorter to detect individual Raman vibrational bands from single particles and sort these particles based on their optical signature. In Years 2-5, we will develop the ability to collect and analyze complete Raman spectra from single particles. In parallel, the partnership will develop new encoding and reporting strategies for multiplexed molecular analysis and separation. This Raman Flow Cytometry technology will be applied to the development of therapeutics and diagnostics for bacterial pathogens and their toxins. Raman Flow Cytometry will be an important and general new analytical and separation capability that will impact many areas of basic and applied biomedical research in addition to the applications proposed here. This collaborative project drives Core R&D Projects 2 (Molecular Assembly) and 4 (Spectral Flow Cytometry).

该子项目是利用资源的众多研究子项目之一 由 NIH/NCRR 资助的中心拨款提供。子项目的主要支持 并且子项目的首席研究员可能是由其他来源提供的， 包括其他 NIH 来源。 子项目可能列出的总成本 代表子项目使用的中心基础设施的估计数量， NCRR 赠款不直接向子项目或子项目工作人员提供资金。 对生物系统进行定量、高通量分子测量的能力是生物医学研究许多领域的关键需求。该生物工程研究合作伙伴关系 (BRP) 旨在开发一个强大的新型分析平台，用于基于拉曼流式细胞术的高通量筛选和选择。该合作伙伴关系将开发新的分析仪器、用于化学合成和筛选的光学编码聚合物树脂，以及用于敏感报告和编码的具有独特光学特性的纳米结构材料。这项新技术将对流动中的单个颗粒进行拉曼光谱分析，以实现灵敏的多重检测、药物发现和诊断方面的新应用。拉曼流式细胞术仪器和应用程序将由来自学术界、政府和工业界的工程师、生物学家和化学家组成的合作伙伴关系开发。在合作伙伴关系的第一年，我们将修改商用颗粒分选机，以检测单个颗粒中的单个拉曼振动带，并根据这些颗粒的光学特征对这些颗粒进行分类。在第 2-5 年，我们将开发收集和分析单个粒子的完整拉曼光谱的能力。与此同时，该合作伙伴关系将为多重分子分析和分离开发新的编码和报告策略。这种拉曼流式细胞术技术将应用于细菌病原体及其毒素的治疗和诊断的开发。拉曼流式细胞术将成为一种重要且通用的新型分析和分离功能，除了此处提出的应用之外，还将影响基础和应用生物医学研究的许多领域。该合作项目推动了核心研发项目 2（分子组装）和 4（光谱流式细胞术）。