MULTIMODALITY HEART PHANTOM MOTION VALIDATION STUDIES

多模态心脏幻影运动验证研究

• 批准号: 8363154
• 负责人: PANOS P CONSTANTINIDES
• 金额: $ 1.84万
• 依托单位: DUKE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-07-01 至 2012-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8363154
• 关键词: Air; Animals; Area; Collaborations; Development; Electrocardiogram; Environment; Ethics; Female; Fiber Optics; Funding; Grant; Heart; Heart Rate; Heating; IACUC; Image; International; Investigation; Isoflurane; Magnetic Resonance Imaging; Maps; Microscopy; Modality; Monitor; Motion; Mus; Myocardial; National Center for Research Resources; Nose; Oxygen; Performance; Phase; Physiologic pulse; Preparation; Principal Investigator; Relaxation; Research; Research Infrastructure; Resolution; Resources; Retinal Cone; Source; Supine Position; System; Systolic Time Intervals; Temperature; Time; United States National Institutes of Health; Universities; Validation; Weight; Work; cost; design; in vivo; instrument; male; multimodality; programs; reconstruction; rectal; seal; validation studies

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. AIM Investigation of the spatial, temporal resolution, and SNR requirements for the optimization, validation and estimation of: i. Myocardial Motion and Eulerian strain using a multimodality realistic heart phantom using DENSE MRI ii. Myocardial strain from the in vivo mouse heart using DENSE MRI METHODOLOGY Animal Preparation and Monitoring: Mice (male/female, CB57L, CD-1, or FVB strains, weightH20-30g) will be induced using 5% isoflurane in a sealed chamber for approximately 3 minutes. The mice will then be mechanically ventilated through a nose cone (with a mixture of 1-2% isoflurane and 100% oxygen to effect), and placed in the supine position in the middle of either a birdcage transmit excitation coil or/and a receive coil, in a specially designed cradle. ECG leads will be attached to the paws and connected to a commercially available fiber optic recording system (SA Instruments Inc.) for monitoring electrical activity and heart rate. A rectal probe will be used to monitor temperature. Air pipes with circulating heated air at a set temperature of 37-38oC will maintain the animals at normal temperature levels. All studies will be performed in accordance with the rules of ethical conduct of research at Duke University and the Institutional Animal Care and Use Committee. DENSE Pulse Sequence Development and Optimization: Implementation of the pulse sequence (PSD) will be achieved by programming in the Excite EPIC environment (EPIC, General Electric, USA), as described in a separate Targeted International Collaboration proposal. In the proposed work, the pulse sequence performance will be assessed and validated in the multi-modality moving phantom and in mice, though phase map reconstructions, optimization of encoding gradients and crusher/spoiler strength-time areas (encoding strength [Genc], time duration [tenc] product), repetition (TR), echo times (TE), and mixing times (TM) for optimal T1-relaxation weighting. Application in in-vivo mouse imaging and optimization of the same parameters for maximum SNR and CNR performance will be conducted in mice, considering its ultra short systolic time intervals (20-30% of the total R-R interval).

该副本是利用资源的众多研究子项目之一 由NIH/NCRR资助的中心赠款提供。对该子弹的主要支持 而且，副投影的主要研究员可能是其他来源提供的 包括其他NIH来源。 列出的总费用可能 代表subproject使用的中心基础架构的估计量， NCRR赠款不直接向子弹或副本人员提供的直接资金。 目的 对空间，时间分辨率和SNR的调查，以优化，验证和估计： 我。使用多模式逼真的心脏幻象使用密集的MRI使用多模式的心肌运动和欧拉尔菌株 ii。使用密集的MRI从体内小鼠心脏的心肌菌株 方法论 动物制备和监测：小鼠（男性/女性，CB57L，CD-1或FVB菌株，重20-30g）将在密封室中使用5％异氟烷诱导大约3分钟。然后，小鼠将通过鼻锥机械通风（用1-2％的异氟烷​​和100％的氧气进行作用），并将其放置在仰卧位的中间，要么是鸟笼传输激发线圈或/和一个接收盘线圈，在专门设计的摇篮中。 ECG潜在客户将连接到爪子上，并连接到商业可用的光纤记录系统（SA Instruments Inc.），以监视电活动和心率。直肠探针将用于监测温度。在37-38oc的设定温度下具有循环加热空气的空气管将使动物保持正常温度水平。所有研究将根据杜克大学和机构动物护理和使用委员会的研究规则进行。 密集的脉冲序列的开发和优化：将通过在一个单独的有针对性的国际协作建议中所述，通过在Excite Epic环境（Epic，General Electric，USA）中进行编程来实现脉冲序列（PSD）的实现。在拟议的工作中，脉冲序列性能将在多模式的移动幻影和小鼠中进行评估和验证，尽管相位图的重建，编码梯度的优化以及破碎机/扰流板/扰流板/扰流板/扰流板的强度时间[GENC]，时间[GENC]，时间[GENC]，时间[GENC]，时间[GENC]持续时间[tenc]产品），重复（TR），回声时间（TE）和混合时间（TM），以实现最佳的T1 - 拉伸权重。考虑到其超短的收缩时间隔（占R-R总间隔的20-30％），将在小鼠中进行最大SNR和CNR性能的相同参数的优化和相同参数的优化。