PHYSIOLOGICAL MECHANISMS OF MYOCARDIAL FORCE GENERATION

心肌力量产生的生理机制

• 批准号: 8171606
• 负责人: PANOS P CONSTANTINIDES
• 金额: $ 1.09万
• 依托单位: DUKE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-07-01 至 2011-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8171606
• 关键词: Agriculture; Anesthesia procedures; Animal Experimentation; Cardiac; Computer Retrieval of Information on Scientific Projects Database; Cyprus; Development; Dose; Ethics; European; Female; Funding; Generations; Genetic; Gills; Grant; Heart; Housing; IACUC; Image; Institutes; Institution; Laboratories; Licensing; Lung; Magnetic Resonance Imaging; Maps; Mechanics; Methodology; Mus; Myocardial; Neurology; Perfusion; Phase; Physiologic pulse; Physiological; Poaceae; Protocols documentation; Radial; Research; Research Personnel; Resources; Rodent; Slide; Source; Testing; United States National Institutes of Health; Universities; Validation; Work; base; male; radiofrequency; reconstruction; research study; simulation

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. AIM: Global mechanical function and perfusion of the mouse heart can be accurately and precisely characterized with strain tensor phase mapping with Displacement Encoding with Stimulated Echoes and radial projection imaging, under optimal anesthesia types and doses, using MRI. WORK PACKAGES -- Development and Testing of Radiofrequency (RF) coils for Cardiac MRI studies. -- Development of a DENSE pulse sequence for cardiac functional mouse studies. -- Development of a comprehensive cardiac function for high-throughput acquisitions in normal mice under anesthesia using MRI. METHODOLOGY: For MRI studies, pulse sequences, imaging protocols and hardware tests, will be validated with simulations and in experiments using phantoms and mice at CIVM-Duke 290 normal mice (male and female, CB57L, CD-1, and FVB strains, weightH20-30g) will be used for the studies of [WP 3-4, 5-8], at LBI and CIVM. For the LBI studies, mice will be purchased from the Cyprus Institute of Neurology and Genetics or from The Jackson Laboratory, USA. Additional mice will be ordered and used at CIVM for testing and validation of the proposed cardiac MRI studies. All studies will be performed in accordance with the rules of ethical conduct of research at Duke University and the Institutional Animal Care and Use Committee, and in accordance with national rules set by the Ministry of Agriculture in Cyprus and according to the European Animal Research directives. The project coordinator and the LBI are fully licensed by the Ministry of Agriculture in Cyprus to conduct the proposed experimental work with mice Pulse Sequence Development and Testing: The 7.0 Tesla scanner (on an Excite GE operating console) will be used for implementation, simulation, and testing of hardware (birdcage transmit [TX]/receive [RX] coil, and RX phased array) and the DENSE/perfusion pulse sequences. A DENSE sequence will be implemented in-house, and tested on the simulator, and on stationary phantoms, and mice. A dynamic contrast enhanced MRI perfusion pulse sequence, previously implemented for pulmonary perfusion in rodents at CIVM, will be modified for use in cardiac mice studies. The sequence is based on interleaved radial imaging and a 'sliding window keyhole reconstruction'. Other commercially available pulse sequences will be tested and optimized for mouse cardiac functional studies, including Radial, Carr Purcell Meiboom Gill (CPMG), Spoiled Grass [SPGR], and FLASH type sequences.

该副本是利用众多研究子项目之一 由NIH/NCRR资助的中心赠款提供的资源。子弹和 调查员（PI）可能已经从其他NIH来源获得了主要资金， 因此可以在其他清晰的条目中代表。列出的机构是 对于中心，这不一定是调查员的机构。 目的：在最佳的麻醉类型和剂量下，使用MRI，可以通过应变张量相位映射与应变张量相位映射的全局机械功能和灌注精确地以应变张量相位映射和径向投影成像进行编码。 工作包 - 用于心脏MRI研究的射频（RF）线圈的开发和测试。 - 开发用于心脏功能小鼠研究的致密脉冲序列。 - 使用MRI在麻醉下，开发正常小鼠中高通量采集的全面心脏功能。 方法论：对于MRI研究，脉冲序列，成像协议和硬件测试将通过模拟验证，并在使用Phantoms和Civm-Duke的幻像和小鼠的实验中进行验证 在LBI和CIVM上，将使用290只普通小鼠（男性和雌性，CB57L，CD-1和FVB菌株，称重20-30G）进行[WP 3-4、5-8]的研究。对于LBI研究，将从塞浦路斯神经病学研究所或美国杰克逊实验室购买小鼠。将在CIVM订购和使用其他小鼠，以测试和验证拟议的心脏MRI研究。所有研究将根据杜克大学和机构动物护理委员会的研究规则，以及根据塞浦路斯农业部制定的国家规则以及欧洲动物研究指令规定的国家规则。该项目协调员和LBI已由塞浦路斯农业部完全许可，以与小鼠进行拟议的实验工作 脉冲序列开发和测试：7.0 Tesla扫描仪（在激发GE操作控制台上）将用于硬件（Birdcage Transmit [TX]/接收[RX] COIL和RX pHASE阵列）和密集/灌注脉冲序列的实现，仿真和测试。将在内部实施密集的序列，并在模拟器，固定幻影和小鼠上进行测试。先前用于CIVM啮齿动物肺灌注的动态对比增强的MRI灌注脉冲序列将被修改用于心脏小鼠研究。该序列基于交织的径向成像和“滑动窗口钥匙孔重建”。其他市售的脉冲序列将进行测试和优化，以用于小鼠心脏功能研究，包括径向，Carr Purcell Meiboom Gill（CPMG），变质的草[SPGR]和Flash型序列。