MECHANICAL TISSUE CHARACTERIZATION AND STRESS / STRAIN IMAGING OF MURINE HEART

小鼠心脏的机械组织特征和应力/应变成像

• 批准号: 8363222
• 负责人: PANOS P CONSTANTINIDES
• 金额: $ 0.31万
• 依托单位: DUKE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-07-01 至 2012-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8363222
• 关键词: Anatomy; Atomic Force Microscopy; Behavior; Cardiac; Characteristics; Computational Technique; Elasticity; Elastomers; Funding; Grant; Heart; Human; Hydrogels; Image; Laws; Magnetic Resonance Imaging; Mechanics; Methodology; Microscopy; Modeling; Morphology; Mus; National Center for Research Resources; Organ; Principal Investigator; Property; Research; Research Infrastructure; Resources; Source; Stress; Tensile Strength; Tissue Engineering; Tissues; United States National Institutes of Health; base; chemical synthesis; cost; density; in vivo; manufacturing process; mouse model; multimodality; programs; shear stress; two-dimensional

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. The specific scientific and technological objectives of this program are: A. Image based modeling and rapid prototyping manufacturing processes can produce two-dimensional (2D) and three-dimensional (3D) tissue mimicking models of the mouse and human cardiac anatomy. B. Standard chemical synthesis methodologies can produce engineered tissue mimicking materials (elastomers, hydrogels) that match the morphology and emulate the in vivo murine and human cardiac tissue mechanical and imaging characteristics. C. Mechanical properties of tissue engineered and murine cardiac myofibers can be accurately and precisely characterized ex-vivo. Specifically: C.1 There are non-significant differences between strain, stress, shear modulus of elasticity, Poisson ratio, density estimates, maximum force and tensile strength developed, of the synthesized tissue mimicking materials and the murine heart C.2 There are significant differences between local (tissue) and global (organ) estimates of tissue elasticity of the fixed murine heart, determined by atomic force microscopy D. Constitutive law behavior of engineered tissue mimicking materials and the in vivo heart, can be accurately and precisely computed with model-based 3D- and 4D-computational techniques using MRI, and validated under dynamic conditions using a multimodality cardiac phantom and in vivo.

该子项目是利用资源的众多研究子项目之一 由 NIH/NCRR 资助的中心拨款提供。子项目的主要支持 并且子项目的首席研究员可能是由其他来源提供的， 包括其他 NIH 来源。 子项目可能列出的总成本 代表子项目使用的中心基础设施的估计数量， NCRR 赠款不直接向子项目或子项目工作人员提供资金。 该计划的具体科技目标是： 答：基于图像的建模和快速原型制造流程可以生成小鼠和人类心脏解剖结构的二维 (2D) 和三维 (3D) 组织模仿模型。 B. 标准化学合成方法可以生产工程组织模拟材料（弹性体、水凝胶），其与形态相匹配并模拟体内小鼠和人类心脏组织的机械和成像特征。 C. 组织工程和小鼠心肌纤维的机械特性可以在体外准确且精确地表征。具体来说： C.1 合成的组织模拟材料和小鼠心脏的应变、应力、弹性剪切模量、泊松比、密度估计、最大力和拉伸强度之间不存在显着差异 C.2 通过原子力显微镜确定的固定鼠心脏组织弹性的局部（组织）和整体（器官）估计之间存在显着差异 D. 工程组织模拟材料和体内心脏的本构律行为可以通过基于模型的 3D 和 4D 计算技术使用 MRI 进行准确和精确的计算，并在动态条件下使用多模态心脏模型和体内进行验证。