ASSESSMENT OF MURINE CARDIAC FUNCTION USING MR IMAGING

使用 MR 成像评估小鼠心脏功能

• 批准号: 7956896
• 负责人: PANOS P CONSTANTINIDES
• 金额: $ 1.09万
• 依托单位: DUKE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-07-01 至 2010-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7956896
• 关键词: Address; Agonist; Anesthesia procedures; Animals; Area; Arts; Calcium; Cardiac; Cardiomyopathies; Cardiovascular Diseases; Cardiovascular system; Catheterization; Cell physiology; Computer Retrieval of Information on Scientific Projects Database; Cues; Cyprus; Development; Disease Management; Employment Opportunities; Europe; European; Funding; Gene Transfer Techniques; Generations; Genetic; Goals; Grant; Heart; Heart failure; Human; Image; Institution; Interdisciplinary Study; Intervention; Knowledge; Lead; Magnetic Resonance Imaging; Mechanics; Mediating; Metabolism; Microscopy; Modeling; Molecular; Mus; Myocardial; Myocardial Contraction; Nature; Organ; Perfusion; Physiology; Property; Quality of life; Relaxation; Research; Research Personnel; Resources; Role; Services; Signal Transduction; Societies; Source; Structure; Techniques; Technology; Time; Tissue Engineering; Tissues; Training Programs; Transgenic Organisms; United States National Institutes of Health; Work; analog; bioimaging; cardiac depression; comparative; depressive symptoms; functional genomics; health care delivery; hemodynamics; improved; man; mouse model; multidisciplinary; novel; skills; tool development

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. This project aims to elucidate mechanisms of myocardial force generation and relaxation in mice through (a) the study of the cardio-depressive effects of inhalational anesthesia at the cellular and tissue levels; (b) the hemodynamic assessment of global mechanical function using invasive catheterization techniques in normal states and with the use of inotropic agents, that include calcium analogs or intracellular agonists; and (c) non-invasive characterization of the murine cardiac function (strain tensor imaging) using Magnetic Resonance Imaging (MRI). All such aims will contribute in part to the complete characterization of the mouse model as a potentially useful model (structure-function, metabolism, perfusion) for extrapolation of scientific conclusions to man, and will ultimately allow comparative analyses of functional genomics in the mouse heart. Collectively, this project aims to develop the technological platform that will allow detailed studies of the cardiovascular system at the molecular, cellular, tissue and organ levels, addressing fundamental questions of the role of genetic cues in human cardiovascular disease, establishing at the same time the scientific platform for the development of a state-of-the-art animal imaging facility in Cyprus. In addition to the scientific value, the value for health care delivery and disease management, such a project will stimulate mobility efforts between USA, Europe and Cyprus and help attract investigators for the pursuit of such work, enable access opportunities to expertise and resources, and establish training programs polarised towards the transfer of knowledge from the USA to Europe and Cyprus, improving the skills and knowledge of European researchers. In all cases novel and superior products to existing ones, new employment opportunities in the high-technology sector would also offer a direct service to society, scientifically and through improvements in the value of the health care delivery and quality of life. The technical research and broader mobility and societal goals will be pursued through this proposed project, within the context of the analysis of the cardiodepressive effects of anesthesia on the animal physiology and myocardial contraction and relaxation, the study of global mechanical function and material properties of tissue at normal states and after positive inotropic intervention of calcium analogs or agonists. Technically, the project is targeted to address its objectives through the proposed research work in the elucidation of mechanisms of cardiac function and the application of pharmacological inotropic agents, as well as the direct use of the developed techniques in the study of mice with transgenic changes. Also, due to its multidisciplinary nature in physiology, cellular signalling, transgenesis, tissue engineering and bioimaging, multidisciplinary research efforts will be stimulated in the respective areas for the development of tools and techniques to study function, to analyse cellular mechanisms that mediate cardiodepression,, and to identify and study critical genetic foci that lead to prominent cardiomyopathies and heart failure.

该副本是利用众多研究子项目之一 由NIH/NCRR资助的中心赠款提供的资源。子弹和 调查员（PI）可能已经从其他NIH来源获得了主要资金， 因此可以在其他清晰的条目中代表。列出的机构是 对于中心，这不一定是调查员的机构。 该项目旨在通过（a）研究细胞和组织水平上吸入麻醉的心脏抑制作用，以阐明小鼠心肌产生和松弛的机制； （b）使用正常状态中的浸润性导管技术对全球机械功能的血液动力学评估以及使用肌力剂的使用，包括钙类似物或细胞内激动剂； （c）使用磁共振成像（MRI）对鼠心脏功能（应变张量成像）的非侵入性表征。 所有此类目标将部分促进小鼠模型作为一种潜在有用的模型（结构功能，代谢，灌注），以推断对人的科学结论，并最终允许对小鼠心脏中功能基因组学的比较分析。总的来说，该项目旨在开发技术平台，该平台将允许在分子，细胞，组织和器官水平上对心血管系统进行详细研究，从而解决遗传提示在人类心血管疾病中的作用的基本问题，并在同一时间建立了在Cyprus中典型动物成像设施开发的科学平台。除科​​学价值外，这种项目的价值还将刺激美国，欧洲和塞浦路斯之间的流动性工作，并帮助吸引调查人员从事此类工作，使访问机会获得专业知识和资源的机会，并建立培训计划，以极化知识从美国转移到欧洲和塞浦路斯，改善了欧洲研究人员的技能和知识。在所有情况下，新颖的产品与现有产品相比，高科技领域的新就业机会也将通过科学地为社会提供直接服务，并通过改善医疗保健提供和生活质量的价值来提供。在分析麻醉对动物生理学和心肌收缩的心脏抑制作用的背景下，将通过该拟议的项目实现技术研究，更广泛的流动性和社会目标，研究正常状态和钙抑制钙类似物的正常状态的全球机械功能和材料的研究。从技术上讲，该项目旨在通过拟议的研究工作来解决其目标，以阐明心脏功能机理和药理学肌力肌体功能的应用，以及在具有转基因变化的小鼠研究中直接使用开发的技术。同样，由于其在生理学上的多学科性质，细胞信号，转基因，组织工程和生物成像，多学科研究工作将在各个领域刺激，以开发研究功能的工具和技术，以分析细胞机制，以介导心脏地位抑制心脏地位，并介导关键的遗传性局部性局部性疾病和心脏病。