MOLECULAR BIOLOGY/VIRAL VECTOR CORE

分子生物学/病毒载体核心

• 批准号: 8360465
• 负责人: DAVID J POULSEN
• 金额: $ 10.42万
• 依托单位: UNIVERSITY OF MONTANA
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-06-01 至 2013-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8360465
• 关键词: Axon; Centers of Research Excellence; Cloning; Computer software; Core Facility; Environmental Health; Equipment; Funding; Gene Expression; Genomics; Genotype; Grant; Molecular Biology; National Center for Research Resources; Pathway interactions; Principal Investigator; Production; Research; Research Infrastructure; Resources; Reverse Transcriptase Polymerase Chain Reaction; Source; Techniques; Time; United States National Institutes of Health; Viral Vector; cost; instrument

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. The Molecular Biology Core Facility (MBCF) was formed during the COBREII funding period from the consolidation of two other cores, the Viral Vector and Microarray cores. It has provided users with equipment and expertise in a wide range of techniques, including gene expression analysis, genotyping, cloning, viral vector production, and basic molecular biology. Instruments and equipment currently available in the facility include: + Agilent (Stratagene) Mx3005p Real-time PCR Instrument + Agilent 2100 Bioanalyzer + Axon GenePix 4000B Microarray Scanner + NanoDrop 1000D Spectrophotometer + MJ Research PTC-100 Thermal Cycler + Eppendorf 5810R Refrigerated Centrifuge + Forma Scientific -80¿C Freezer + Roche Universal ProbeLibrary for RT-PCR + Ariadne Genomics Pathway Studio Software The MBCF has been directed by Dr. David Poulsen, and staffed by Corbin Schwanke. Over the past year funding for the core has been solely provided by the CEHS COBRE grants for the Center for Environmental Health Sciences.

该副本是利用资源的众多研究子项目之一 由NIH/NCRR资助的中心赠款提供。对该子弹的主要支持 而且，副投影的主要研究员可能是其他来源提供的 包括其他NIH来源。列出的总费用可能 代表subproject使用的中心基础架构的估计量， NCRR赠款不直接向子弹或副本人员提供的直接资金。 分子生物学核心设施（MBCF）是在COBREII资金期间由其他两个核心（病毒载体和微阵列核心的合并）形成的。它为用户提供了各种技术的设备和专业知识，包括基因表达分析，基因分型，克隆，病毒载体产生和基本分子生物学。该设施中当前可用的仪器和设备包括： + Agilent（Stratagene）MX3005P实时PCR仪器 + Agilent 2100生物分析仪 + Axon Genepix 4000B微阵列扫描仪 + Nanodrop 1000D分光光度计 + MJ Research PTC-100热循环器 + Eppendorf 5810R冷藏离心机 + Forma Scientific-80¿CFreezer + ROCHE通用概率RT-PCR + Ariadne Genomics Pathway Studio软件 MBCF由David Poulsen博士执导，由Corbin Schwanke组成。在过去的一年中，核心的资金仅由CEHS COBRE赠款为环境健康科学中心提供。