MOLECULAR BIOLOGY/VIRAL VECTOR CORE

分子生物学/病毒载体核心

• 批准号: 8167595
• 负责人: DAVID J POULSEN
• 金额: $ 10.48万
• 依托单位: UNIVERSITY OF MONTANA
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-06-01 至 2011-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8167595
• 关键词: Computer Retrieval of Information on Scientific Projects Database; Core Facility; Equipment; Funding; Gene Expression; Genes; Genotype; Grant; Institution; Mass Spectrum Analysis; Methods; Molecular Biology; Molecular Biology Techniques; Montana; Nucleic Acids; Production; Proteomics; Reporter; Research; Research Personnel; Resources; Reverse Transcriptase Polymerase Chain Reaction; Services; Source; Two-Dimensional Gel Electrophoresis; United States National Institutes of Health; Universities; Viral Vector; instrument; overexpression

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The Molecular Biology Core Facility was created in 2008 by combining the equipment and expertise from two under-utilized cores at The University of Montana. Utilizing the instruments and staff from the Microarray Core Facility and the Viral Vector Core Facility, this new core covers the entire spectrum of molecular biology techniques and applications. The main services provided by the core are: 1) Gene expression analysis by RT-PCR and microarray, 2) Proteomics analysis by 2D gel electrophoresis and mass spectrometry, 3) Genotyping by a variety of methods, 4) Viral vector production for gene overexpression, gene knockdown, and reporter expression, 5) Assistance with basic molecular biology techniques, such as nucleic acids isolation and PCR.

该副本是利用众多研究子项目之一 由NIH/NCRR资助的中心赠款提供的资源。子弹和 调查员（PI）可能已经从其他NIH来源获得了主要资金， 因此可以在其他清晰的条目中代表。列出的机构是 对于中心，这不一定是调查员的机构。 分子生物学核心设施是通过结合蒙大拿大学两个未充分利用的核心的设备和专业知识来创建的。 该新核心利用微阵列核心设施和病毒矢量核心设施的工具和人员涵盖了整个分子生物学技术和应用。 核心提供的主要服务是：1）通过RT-PCR和微阵列进行的基因表达分析，2）通过2D凝胶电泳和质谱法分析蛋白质组学分析，3）3）通过多种方法进行基因分型，4）基因过表达，基因敲低和依赖基本酸的基因构图，5）隔离物质的基因过表达，基因敲低，5）隔离学技术，例如，peccr and ccr and ccr sectre and ccr secral precer precer precer precer secriques secriques sekiques，例如。