Molecular Biology Core

分子生物学核心

• 批准号: 10618376
• 负责人: Jon Scott Blevins
• 金额: $ 22.8万
• 依托单位: UNIV OF ARKANSAS FOR MED SCIS
• 依托单位国家: 美国
• 财政年份: 2022
• 资助国家: 美国
• 起止时间: 2022-05-05 至 2027-04-30
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10618376
• 关键词: Address; Antibody Formation; Applications Grants; Arkansas; Award; Centers of Research Excellence; Cloning; Core Facility; DNA; DNA Sequencing Facility; DNA biosynthesis; DNA sequencing; Data; Dedications; Doctor of Philosophy; Ensure; Equation; Equipment; Fee-for-Service Plans; Fees; Flow Cytometry; Funding; Gel; Genes; Genomic Library; Genomics; Goals; Growth; Human Resources; Immunology; Inflammatory; Inflammatory Response; Institution; Investments; Laboratory Personnel; Maintenance; Manuscripts; Medical; Medicine; Methods; Microbial Genome Sequencing; Microbiology; Modernization; Molecular Biology; Opportunistic Infections; Pathogenesis; Phase; Preparation; Proteins; RNA; Recovery; Research; Research Infrastructure; Research Personnel; Resources; Sampling; Science; Scientist; Service delivery model; Services; Side; Supervision; Technology; Time; Training; Universities; Validation; Western Blotting; Work; base; cellular imaging; college; cost; equipment acquisition; genome-wide; instrument; instrumentation; microbial; microscopic imaging; next generation; next generation sequencing; operation; pathogen; programs; protein expression; protein purification; quantitative imaging; response; success; tool; user-friendly; voucher

PROJECT SUMMARY/ABSTRACT In Phases I and II, the Center for Microbial Pathogenesis and Host Inflammatory Responses (CMPHIR) established and maintained a Research and Technical Advancement Core to fill experimental gaps in the resources available in institutional cores maintained by the UAMS College of Medicine. One of these was a DNA Sequencing Core. However, the capability of this core was limited to single-gene sequencing. While this was an important and necessary service, it was not sufficient, particularly in an era of genomic and next-generation sequencing technology. Thus, the CMPHIR also invested heavily in augmenting the resources available in the existing DNA Sequencing Core. We accomplished this task, and, in Phase III, we propose to integrate these services under the common umbrella of a Molecular Biology Core (Core B) capable of providing CMPHIR and other UAMS investigators with the resources they need, particularly with respect to the pathogen side of the host–pathogen equation. The activities of Core B include enhancing the capability of obtaining high-quality RNA, DNA, and protein samples for analysis; high-throughput single-gene sequencing using conventional Sanger methods; next-generation sequencing of microbial genomes and genomic libraries; protein expression and purification; and antibody production. The proposed Core B will be supported through a combination of CMPHIR funding and continued institutional support. Daily operations will be supervised by Dr. Jon S. Blevins, Director, who will work with 2 full-time technicians. One of these technicians will have primary responsibility for the DNA component of Core B, while the other will have primary responsibility for the protein component. To ensure consistency and promote self-sustainability, Core B instruments will be operated by these technicians on behalf of CMPHIR and UAMS investigators on a fee-for-service basis. This will allow us to recover a significant proportion of the costs and thereby ensure the ability to upgrade and maintain the equipment in this core as needed as we move forward into Phase III and beyond.

项目摘要/摘要 在第I和II阶段，微生物发病机理和宿主炎症反应中心（CMPHIR） 建立并维护了研究和技术进步核心，以填补实验空白 UAMS医学院维护的机构核心中可用的资源。其中之一是DNA 测序核心。但是，该核心的能力仅限于单基因测序。虽然这是一个 重要和必要的服务，这还不够，尤其是在基因组和下一代的时代 测序技术。这，CMPHIR还大量投资于增加 现有的DNA测序核心。我们完成了这项任务，在第三阶段，我们建议将这些任务整合在一起 分子生物学核心（核心B）的公共伞下的服务能够提供CMPHIR和 其他UAM调查人员拥有所需的资源，尤其是在病原体方面 宿主 - 病因方程。核心B的活动包括增强获得高质量RNA的能力， DNA和蛋白质样品进行分析；使用常规Sanger的高通量单基因测序 方法;微生物基因组和基因组文库的下一代测序；蛋白表达和 纯化;和抗体产生。提出的核心B将通过CMPHIR组合支持 资金和持续的机构支持。每日操作将由乔恩·S·Blevins博士进行监督 谁将与2位全职技术人员一起工作。这些技术人员之一将对DNA负责 核B的组成部分，而另一个将对蛋白质成分负有主要责任。确保 一致性和促进自我可持续性，核心B工具将由这些技术人员代表操作 CMPHIR和UAMS调查人员的费用为基础。这将使我们能够恢复重要的 成本的比例，从而确保能够升级和维护此核心设备的能力 当我们进入第三阶段及以后时，需要。