COBRE: UNE MED CTR: CORE D: DNA MICROARRAY CORE

COBRE：UNE MED CTR：核心 D：DNA 微阵列核心

• 批准号: 8360393
• 负责人: JAMES D EUDY
• 金额: $ 6.42万
• 依托单位: UNIVERSITY OF NEBRASKA MEDICAL CENTER
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-07-01 至 2012-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8360393
• 关键词: Biological; Biological Process; Centers of Research Excellence; Clinical; Core Facility; DNA Microarray Chip; DNA-Protein Interaction; Data; Development; Diagnosis; Disease; Drosophila genome; Extramural Activities; Funding; Future; Gene Expression; Genetic Variation; Genome; Grant; Human; Medicine; Microarray Analysis; Molecular Biology; Molecular Profiling; Mus; National Center for Research Resources; Outcome; Pathway interactions; Principal Investigator; Relative (related person); Research; Research Infrastructure; Research Personnel; Research Project Grants; Resources; Source; Stimulus; System; Technology; United States National Institutes of Health; cost; design; insight; neurosurgery; new technology; novel; prognostic; research study; response

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. The completion of the sequencing of several different genomes including the human, mouse, and drosophila genomes, has resulted in tremendous opportunities for researchers to make biomedical advances (Waterston et al, 2002; Venter et al, 2001). DNA microarray technology is one of the new technologies that takes advantage of the sequence data and is a powerful highthroughput means to gain crucial insight into both normal and disease-related biological processes (Holloway AJ et al, 2002; Shultz and Downward, 2001). Although the technology has only been developed in roughly the last ten years, it has advanced rapidly and the list of applications in which microarrays have been used is growing. Microarray experiments are capable of comprehensivelyassessing relative changes in global gene expression profiles and as such can be used to elucidate transcriptional mechanisms dictating development and the response to environmental stimuli (lyer et al, 1999). They can also be used to characterize disease states and associate these transcriptional profiles with prognostic outcome (Chung et al, 2002). In addition to DNA microarrays that are designed to assess transcriptional activity, there are DNA microarrays that are designed to characterize DNA / protein interactions as well as genetic variation (Pollack and lyer, 2002; Patil N et al, 2001). It is also predicted that in the future DNA microarrays will be used in clinical settings for diagnosis and perhaps personalized medicine (Petricoin EF et al, 2002). The UNMC DNA Microarray Core Facility will support the COBRE project as outlined by providing the necessary resources for the investigators to utilize DNA microarray technology in their respective research projects. Microarray technology is a means by which much needed novel insights can be gained in biological pathways, as such it will help the junior investigators obtain data to significantly increase the likelihood that they will be competitive in regard to applying for extramural funding.

该副本是利用资源的众多研究子项目之一 由NIH/NCRR资助的中心赠款提供。对该子弹的主要支持 而且，副投影的主要研究员可能是其他来源提供的 包括其他NIH来源。 列出的总费用可能 代表subproject使用的中心基础架构的估计量， NCRR赠款不直接向子弹或副本人员提供的直接资金。 几种不同基因组的测序完成，包括人，小鼠和果蝇基因组，为研究人员带来了巨大的机会 生物医学进步（Waterston等，2002； Venter等，2001）。 DNA微阵列技术是利用序列数据的新技术之一，并且是一种强大的高率手段，可以洞悉正常和疾病相关的生物学过程（Holloway AJ等，2002; Shultz and Downward，2001）。尽管该技术仅在过去十年中开发，但它已经迅速发展，并且使用微阵列的应用程序列表正在增长。微阵列实验能够全面评估全局基因表达谱的相对变化，因此可以用来阐明决定发展的转录机制和对环境刺激的反应（Lyer等，1999）。它们也可以用来表征疾病状态并将其关联 具有预后结果的转录谱（Chung等，2002）。除了旨在评估转录活性的DNA微阵列外，还有DNA微阵列 旨在表征DNA /蛋白质相互作用以及遗传变异（Pollack和Lyer，2002； Patil N等，2001）。还可以预测，将来DNA微阵列将用于 诊断和个性化医学的临床环境（Petricoin EF等，2002）。 UNMC DNA微阵列核心设施将通过为调查人员提供必要的资源来支持毛茸茸的项目，以便研究人员在其各自的研究项目中利用DNA微阵列技术。微阵列技术是一种急需小说的手段 可以在生物途径中获得见解，因此，这将有助于初级研究人员获得数据，以显着增加其在申请申请方面具有竞争力的可能性 壁外资金。