UNMC: MICROARRAY CORE, GENOMICS

UNMC：微阵列核心、基因组学

• 批准号: 7725172
• 负责人: JAMES D EUDY
• 金额: $ 9.77万
• 依托单位: UNIVERSITY OF NEBRASKA MEDICAL CENTER
• 依托单位国家: 美国
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-05-01 至 2009-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7725172
• 关键词: Area; Bacterial Infections; Biological Models; Biological Process; Cell Membrane Permeability; Cells; Chlorella; Communities; Computer Retrieval of Information on Scientific Projects Database; Congenital Heart Defects; Core Facility; Custom; Data; Development; Diabetes Mellitus; Disease; Doctor of Philosophy; Drosophila genus; Equipment; Evolution; Fetal Alcohol Syndrome; Funding; Gene Expression; Gene Expression Regulation; Genes; Genetic Transcription; Genomics; Glaucoma; Grant; HIV; Heart failure; Human; Human Resources; Immune; Immune system; Institution; Knowledge; Laboratories; Listeria; Listeria monocytogenes; Lung diseases; Lymphoma; Malignant Neoplasms; Malignant neoplasm of prostate; Metabolism; Modeling; Molecular; Mus; Nebraska; Nerve Degeneration; Oligonucleotides; Operative Surgical Procedures; Paramecium; Parkinson Disease; Pattern; Pituitary Neoplasms; Printing; Process; Protein Overexpression; Pseudomonas aeruginosa; Reagent; Research; Research Personnel; Resources; Retinal Diseases; Robotics; Services; Signal Transduction; Slide; Source; Spottings; Support of Research; Surveys; United States National Institutes of Health; Universities; Virulence Factors; Virus; Work; alcohol exposure; cancer genetics; college; design; functional genomics; hearing impairment; homoserine lactone; insight; instrumentation; leukemia; malignant breast neoplasm; malignant mouth neoplasm; pathogen; programs; quorum sensing; rat genome; research study

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The Microarray Core Facility has provided service and support to INBRE associates as well as other academic investigators in the State of Nebraska during Year #1 of the INBRE grant. Funds were used to purchase reagents to fabricate and process slides, upgrade microarray specific instrumentation critical to the operations of the facility, and to provide personnel support including 50 % FTE for a research technologist and 15% FTE for the director of the core. The support received from the INBRE program has had a tremendous impact on the ability of the core facility to provide functional genomics support to the academic community. Outlined below are the accomplishments of the core since June of 2004. MICROARRAY SERVICES AND SUPPORT YEAR #4 INBRE Associates: Kim Carlson Ph.D. University of Nebraska at Kearney: The facility has completed a set of microarray experiments (analysis underway) with Dr. Carlson to perform a functional genomics experiment using Drosophila Affymetrix GeneChips. The specific project is determining the gene regulation of developmental and immune genes as a result of OTK18 overexpression using Drosophila as a model system. Doug Christensen PhD-Wayne State: The facility has printed a custom spotted microarray to survey Listeria monocytogenes gene expression. Gene expression experiments will begin soon. This data will be used to further knowledge of virulence factors associated with Listeria and how cells of the human immune system may or may not stimulate unique gene transcription in this pathogen. Barbara Clement PhD-Doane College: The core facility has been working with Dr. Clement designing experiments to study the effect of membrane permeability on the ability of Pseudomonas aeruginosa to detect quorum sensing (QS) signals (N-acyl homoserine lactones or AHL). Experiments are underway. Garry Duncan PhD Nebraska Wesleyan University: The facility has been working with Dr. Duncan designing a custom spotted microarray to survey Paramecium bursaria Chlorella virus-1 (PBCV-1) gene expression. Preliminary printing and subsequent gene expression data is intriguing. This virus is an ancient virus with some atypical molecular mechanisms and thus may yield insight into evolution of metabolism and other biological processes. Kathleen Tallman PhD-Doane College: The facility has been working with Dr. Tallman designing experiments to study changes in gene expression in mice following ethanol exposure. This is intended to serve as a model for fetal alcohol syndrome studies. Non-INBRE Investigators: Spotted Array Platform: The core routinely prints and processes 10K slide sets representing human, mouse and rat genomes using the oligonucleotide sets and robotic spotter purchased with BRIN funds. The Core also prints custom arrays for researchers. In addition to the INBRE users listed above, the facility serviced 20 investigators from four institutions in the State of Nebraska. 15 of these researchers were at UNMC, 3 were at UNL, 1 at UNO, and 1 at Creighton University. Affymetrix Platform: The BRIN supported research technologist in the laboratory processed Affymetrix chips for 21 investigators located at three institutions in Nebraska. These include 19 investigators at UNMC, 1 at UNO, and 1 at Boystown National Research Hosptital. Examples of Research Areas Of Above Investigators: Retinal disease, prostate cancer, lymphoma, Parkinson?s disease, methlyation patterns and cancer, HIV associated neural degeneration, bacterial infection associated disease, leukemia, pulmonary disease, developmental heart defects, heart failure, glaucoma, oral cancer, genetics of hearing loss, pituitary tumors, breast cancer, and diabetes. EQUIPMENT PURCHASES Funds from year #4 were used to purchase 32 new printer pins for the robotic spotter. This is necessary to keep the spotter printing slides with uniform spots.

该副本是利用众多研究子项目之一 由NIH/NCRR资助的中心赠款提供的资源。子弹和 调查员（PI）可能已经从其他NIH来源获得了主要资金， 因此可以在其他清晰的条目中代表。列出的机构是 对于中心，这不一定是调查员的机构。 MicroArray核心设施为Inbre Associates以及内布拉斯加州州的其他学术调查员提供了服务和支持，在Inbre Grant的第一年期间。资金用于购买试剂以制造和处理幻灯片，升级对设施运营至关重要的微阵列特定仪器，并提供人员支持，包括研究技术人员的50％FTE和核心主管的15％FTE。从Inbre计划获得的支持对核心设施为学术界提供功能基因组学支持的能力产生了巨大影响。自2004年6月以来，下面概述了核心的成就。 微阵列服务和支持年4年 Inbre Associates： 金·卡尔森博士内布拉斯加州大学的科尔尼大学：该设施已与卡尔森博士完成了一组微阵列实验（分析），以使用果蝇伴侣基因进行功能基因组学实验。 特定项目是通过使用果蝇作为模型系统的OTK18过表达来确定发育和免疫基因的基因调节。 道格·克里斯滕森（Doug Christensen Phd-Wayne）州：该设施已印刷了一个定制的斑点微阵列，以调查单核细胞增生李斯特菌的基因表达。基因表达实验将很快开始。这些数据将用于进一步了解与李斯特菌有关的毒力因子以及人类免疫系统的细胞如何刺激该病原体中独特的基因转录。 芭芭拉·克莱门特（Barbara Clement）博士学位学院（Barbara Clement Phd Doane College）：核心设施一直在使用克莱门特（Clement）设计实验，以研究膜渗透性对铜绿假单胞菌检测群体传感（QS）信号（N-酰基同胺lactones或AHL）的能力的影响。 实验正在进行中。 加里·邓肯（Garry Duncan）博士内布拉斯加州卫斯理大学（Nebraska Wesleyan University）：该设施一直与邓肯博士合作，设计了定制的斑点微阵列，以调查parmecium bursaria bursaria bursaria chlorella virus-1（PBCV-1）基因表达。初步打印和随后的基因表达数据很有趣。该病毒是一种古老的病毒，具有一些非典型的分子机制，因此可能会深入了解新陈代谢和其他生物过程的进化。 凯瑟琳·塔尔曼（Kathleen Tallman）博士学位学院（Kathleen Tallman Phd-Doane College）：该设施一直在与Tallman Designers Design实验合作，研究乙醇暴露后小鼠中基因表达的变化。这旨在作为胎儿酒精综合征研究的模型。 非疾病调查员： 斑点阵列平台：核心通常使用寡核苷酸套件和用Brin资金购买的寡核苷酸套件和机器人摄影仪，代表人，鼠标和大鼠基因组的核心幻灯片集。核心还为研究人员打印自定义阵列。除了上面列出的近亲用户外，该工厂还为内布拉斯加州四家机构的20位调查人员提供服务。这些研究人员中有15名在UNMC，其中3个在UNL，1个在UNO，1名在Creighton University。 Affymetrix平台：BRIN在实验室处理的Affymetrix芯片中为21位位于内布拉斯加州三个机构的研究人员提供了研究技术专家。其中包括19名在UNMC的调查员，UNO的1名调查员，在Boystown国家研究霍斯蒂塔尔（Boystown National Research Hosttital）中包括1名调查员。 Examples of Research Areas Of Above Investigators: Retinal disease, prostate cancer, lymphoma, Parkinson?s disease, methlyation patterns and cancer, HIV associated neural degeneration, bacterial infection associated disease, leukemia, pulmonary disease, developmental heart defects, heart failure, glaucoma, oral cancer, genetics of hearing loss, pituitary tumors, breast cancer, and diabetes. 设备购买 ＃4年级的资金用于购买32个新的打印机销供机器人侦察员使用。 这是保持均匀景点的点幻灯片幻灯片的必要条件。