Illumina Hi-Seq 2000 Sequencing System

Illumina Hi-Seq 2000 测序系统

• 批准号: 8246554
• 负责人: Gregory A. WRAY
• 金额: $ 59.24万
• 依托单位: DUKE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2012
• 资助国家: 美国
• 起止时间: 2012-05-07 至 2013-05-06
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8246554
• 关键词: Basic Science; Bioinformatics; Biomedical Research; Chromatin; Clinical Research; Communities; Consultations; Core Facility; DNA; DNA Sequence; DNA-Protein Interaction; Ensure; Equipment; Funding; Gene Expression Profile; Generations; Genome; Libraries; Methylation; Preparation; Quality Control; Reading; Research Infrastructure; Research Personnel; Research Project Grants; Running; Sampling; Sequence Analysis; Services; Site; System; Time; United States National Institutes of Health; Universities; base; catalyst; cost; experience; genome sequencing; instrument; instrumentation; meetings; next generation

DESCRIPTION (provided by applicant): A new generation of ultra high-throughput DNA sequencers is transforming biomedical research. These instruments provide a wide range of applications to basic and clinical research, including SNP discovery, analysis of transcriptome profiles, identification of protein:DNA interactions and hypersensitive sites, and characterization of methylation and chromatin marks at the whole-genome scale. This proposal seeks funds to expand instrumentation within Duke University's Genome Sequencing & Analysis Core Facility by adding a HiSeq" 2000 System next-generation DNA sequencer manufactured by Illumina. Existing next-generation capabilities within the Core Facility include four short-read instruments (three Illumina GAII" and one Applied Biosystems SOLiD" 4) and one long-read instrument (Roche GS-FLX"). We acquired our first GAII" three years ago and replacing this instrument with the new HiSeq" 2000 would substantially expand capacity to meet growing demand and extend the range of services that the Core Facility provides to researchers. The HiSeq" platform offers both a substantially larger throughput and a faster run time. Access to this instrument will therefore save some research projects time and cost, and provide others with expanded information. This instrument will be primarily utilized by eight identified Duke investigators for diversity of ongoing projects. Because of a pre-existing strong Illumina user base in the Core Facility and the HiSeq" versatility, we anticipate that this new instrument will enable a wide range of additional projects over its lifetime. Efficient utilization of the instrument is ensured y placement in a Core Facility with a proven record of accomplishment of more than a decade of support for research projects. Since this new instrument would replace an older Illumina instrument, its integration to an established workflow would be easily achieved and no additional ancillary equipment, staff, and computing infrastructure would be required. The Core Facility provides start-to- finish partnership with investigators that includes consultation, sample preparation, library construction, DNA sequencing, post-run quality control, and in-depth bioinformatics and statistical analyses. A team of technicians and bioinformaticians with strong experience in operating various next-generation DNA sequencers would support the new instrument, allowing non-specialists to take immediate advantage of its capabilities. A HiSeq" 2000 DNA sequencer would provide substantial benefit to several ongoing NIH-funded projects and serve as a valuable catalyst for additional projects throughout Duke University's vibrant biomedical research community.

描述（由申请人提供）：新一代的超高通量DNA测序仪正在改变生物医学研究。这些仪器为基本和临床研究提供了广泛的应用，包括SNP发现，转录组轮廓分析，蛋白质的鉴定：DNA相互作用和过敏部位以及表征 甲基化和染色质标记在整个基因组尺度上。该提案通过添加Illumina制造的HISEQ“ 2000系统下一代DNA Sequencer。核心设施中现有的下一代功能包括四个短读仪器（包括四个简短阅读工具）（三个Illumina gaii”（三个Illumina gaii''和一个applied bioSystems Sield off gs gs gs gs gs gs gs gs gs gs gs gs gs gs gs gs gs gs gs gs gs gs gs gs gs gs gs gs gs gs gs gs gs，该提案寻求资金来扩展杜克大学基因组测序和分析核心设施中的仪器。我们获得了我们的第一个Gaii“三年前，并用新的Hiseq取代了2000年”将大大扩大能力，以满足不断增长的需求，并扩展了核心设施为研究人员提供的服务范围。 HISEQ“平台既可以提供更大的吞吐量，又提供更快的运行时间。访问此乐器将 因此，节省了一些研究项目的时间和成本，并为其他人提供扩展的信息。该工具将主要由八名已确定的杜克大学调查人员用于正在进行的项目的多样性。由于核心设施中有一个预先存在强大的光明用户基础，“多功能性，我们预计这种新工具将在其一生中能够在核心设施中有效地利用该仪器的高效利用，从而确保在核心设施中置于核心设施中，而既可以替代了一项新的工具，就可以替换了一项新的仪器，因为它可以替换一项新的工具。需要其他辅助设备，人员和计算基础设施。立即利用其功能。 HISEQ“ 2000 DNA Sequencer将为几个正在进行的NIH资助的项目提供可观的好处，并为杜克大学充满活力的生物医学研究社区的其他项目提供宝贵的催化剂。