Integrated Cancer Genomics Core

综合癌症基因组学核心

• 批准号: 10544823
• 负责人: Gregory A. WRAY
• 金额: $ 46.43万
• 依托单位: DUKE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1997
• 资助国家: 美国
• 起止时间: 1997-01-01 至 2024-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10544823
• 关键词: Accounting; Advanced Malignant Neoplasm; Alternative Splicing; Binding Sites; Bioinformatics Shared Resource; Biological Assay; Cancer Center; Cancer Center Support Grant; Cells; Clinical; Clinical Trials; Collaborations; Computational Biology; Consultations; Core Facility; Correlative Study; DNA Methylation; Data Analyses; Databases; Development; Education; Educational workshop; Ensure; Epigenetic Process; Event; Experimental Designs; Fees; Formalin; Genes; Genomics; Genomics Shared Resource; Goals; Grant; Guidelines; Immune system; Information Dissemination; Infrastructure; Institution; Journals; Knowledge; Learning; Malignant Neoplasms; Manuscripts; Maps; Measures; Messenger RNA; Molecular; Paraffin Embedding; Physiology; Preparation; Procedures; Protocols documentation; Publications; Quality Control; Recovery; Research; Research Institute; Research Personnel; Resource Sharing; Resources; Reverse Transcriptase Polymerase Chain Reaction; Sampling; Schedule; Services; Statistical Data Interpretation; Structure; Technology; Time; Training; Training and Education; Update; Vendor; Work; analysis pipeline; anticancer research; cancer genomics; chromatin modification; cost; data management; data quality; equipment acquisition; exome sequencing; experience; flexibility; improved; innovative technologies; instrumentation; medical schools; member; microbial; microbiome; nano-string; neoplastic cell; new technology; novel; programs; research and development; sample collection; single cell sequencing; success; technology platform; tool; transcription factor; transcriptome; transcriptomics

ABSTRACT– INTEGRATED CANCER GENOMICS SHARED RESOURCE The Integrated Cancer Genomics shared resource (ICG) is committed to providing state-of-the-art instrumentation and protocol support to Duke Cancer Institute (DCI) researchers as these technologies evolve over time. The ICG expanded to meet DCI needs that encompass microbiome, epigenetic and increase single cell services provided by three institutionally designated core facilities: Sequencing and Genomic Technologies Core (SGT), the recently established Microbiome Shared Resource (MSR), and the Molecular Genomics Core (MGC). By unifying these existing resources for DCI members, the ICG meets its objective to provide one-stop access to all of the major research protocols and instrumentation platforms used in contemporary cancer genomics research, including genomics, transcriptomics, microbial studies, and epigenetics. For over a decade, the ICG has maintained a record of providing updated, state-of-the-art genomic and transcriptomic services to DCI members. The ICG includes services that are performed by three Duke School of Medicine (SOM) core facilities. The Sequencing and Genomic Technologies (SGT) and Microbiome Shared Resource (MSR) perform services within the Duke Center for Genomics and Computational Biology; the Molecular Genomics Core (MGC) performs services within the Duke Molecular Physiology Institute. The ICG unifies all of the cancer genomic technologies on campus, providing one-stop access to all of the major research protocols and instrumentation platforms used in contemporary cancer genomics research. The ICG supports a wide range of projects from DCI investigators, by providing expert consultation, project management and training to facilitate access to approaches including SNP discovery, mapping chromatin modifications, single-cell sequencing, measuring mRNA levels at several scales (single genes, cancer panels, entire transcriptome), sequencing exomes, identifying DNA methylation, microbiome profiling, and mapping transcription factor binding sites. By offering the full range of technological platforms, the ICG allows investigators to choose the optimal solution for their cancer related projects and assists investigators with data quality control, versioning, statistical analysis, and dissemination for all of these services. In addition, the ICG works with DCI investigators to explore and establish new technologies which catalyzes the advancement of cancer research. In 2018, the ICG shared resource provided services to 290 investigators, 29% of whom were DCI members, accounting for 25% of total usage, from all 8 DCI Research Programs. Use of this shared resource by DCI Members contributed to 216 publications over the project period, 80 of which were in high impact journals. The shared resource operates primarily on a cost-recovery basis, with institutional support for a portion of the operating costs and instrument purchases. Support from the Cancer Center Support Grant allows ICG to provide DCI members with consultations, assistance with grant and manuscript preparation, and scheduling priority. User fees for other activities follow School of Medicine guidelines.

摘要 - 综合癌症基因组共享资源 综合癌症基因组学共享资源（ICG）致力于提供最先进的 随着这些技术的发展，杜克癌症研究所（DCI）研究人员的仪器和协议支持 随着时间的推移。 ICG扩展以满足涵盖微生物组，表观遗传和增加单一的DCI需求 由三个机构指定的核心设施提供的细胞服务：测序和基因组技术 Core（SGT），最近建立的微生物组共享资源（MSR）和分子基因组核心 （MGC）。通过为DCI成员统一这些现有资源，ICG符合其目标的目标 访问当代癌症中使用的所有主要研究方案和仪器平台 基因组学研究，包括基因组学，转录组学，微生物研究和表观遗传学。 十多年来，ICG一直保持提供更新的最新基因组和 DCI成员的转录组服务。 ICG包括由三所杜克学校提供的服务 医学（SOM）核心设施。测序和基因组技术（SGT）和微生物组共享 资源（MSR）在杜克大学基因组学和计算生物学中心内执行服务；这 分子基因组学核心（MGC）在杜克分子生理研究所内执行服务。 ICG 统一的校园中所有癌症基因组技术，可一站式访问所有主要研究 当代癌症基因组学研究中使用的方案和仪器平台。 ICG支持 通过提供专家咨询，项目管理和培训，来自DCI调查人员的广泛项目 为了促进访问包括SNP发现，映射染色质修饰在内的方法，单细胞 测序，测量几个尺度（单基因，癌症面板，整个转录组）的mRNA水平， 测序外体，鉴定DNA甲基化，微生物组分析和映射转录因子结合 站点。通过提供各种技术平台，ICG允许研究人员选择最佳 解决与癌症相关项目的解决方案，并协助研究人员进行数据质量控制，版本控制，统计数据 分析和所有这些服务的传播。此外，ICG与DCI调查人员合作探索 并建立新技术，以促进癌症研究的发展。 2018年，ICG共享资源为290名调查人员提供了服务，其中29％是DCI成员， 从所有8个DCI研究计划中占总用法的25％。 DCI使用此共享资源 成员在项目期间为216个出版物做出了贡献，其中80个是在高影响期刊中。这 共享资源主要基于成本回收的基础运行，并在机构上支持部分 运营成本和工具购买。癌症中心支持赠款的支持使ICG能够提供 DCI成员进行咨询，协助赠款和手稿准备以及计划优先级。用户 其他活动的饲料遵循医学学院指南。