PacBio Sequel IIe for GCB Sequencing Core

用于 GCB 测序核心的 PacBio Sequel IIe

• 批准号: 10630988
• 负责人: Gregory A. WRAY
• 金额: $ 49.81万
• 依托单位: DUKE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2023
• 资助国家: 美国
• 起止时间: 2023-06-15 至 2024-06-14
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10630988
• 关键词: Complement; Complex; DNA; DNA Sequencing Facility; Data; Detection; Discrimination; Disease; Employee; Event; Funding; Genetic Recombination; Genomic Segment; Genomics; Laboratories; Libraries; Methods; North Carolina; Phase; Production; RNA Splicing; Repetitive Sequence; Research Personnel; Resource Sharing; Resources; Services; System; Technology; United States National Institutes of Health; Universities; Update; Virus; cost; cost effective; foot; instrument; instrumentation; medical schools; nanopore; transcriptome sequencing

The Duke Sequencing and Genomics Technology Shared Resource (SGT) serves PIs in the biomedical fields from Duke’s School of Medicine and all over Duke. The SGT is a ~6600 sq foot state-of-the-art facility that houses more than $3.1M of instrumentation and has ten full time employees. For more than a decade, the SGT consistently has provided updated, state-of-the-art genomic services. Like most laboratories, short-read DNA/RNA sequencing is the dominant form of sequencing technology in the SGT because it is highly accurate and cost-effective. However, short-read sequencing cannot access complex genomics regions. To complement short-read sequencing methods, labs including the SGT use long-read sequencing technologies. As such, the SGT provides PIs with highly accurate short Illumina sequencing reads and moderately accurate ultra-long Oxford Nanopore sequencing reads. However, the SGT currently does not offer highly accurate long sequencing reads produced by the PacBio Sequel IIe system. The Sequel IIe, the instrument requested in this proposal, would be a unique resource to Duke University and academic institutes in North Carolina. PacBio has recently overcome weaknesses that have long plagued long-read technology. These weaknesses are inaccuracy, low-throughput production, and high cost. First HiFi libraries sequenced by the PacBio Sequel IIe are highly accurate. Next multiple technologies developed by researchers and PacBio increase the throughput of long-read sequencing. Additionally, the Sequel IIe has local computational abilities that reduces labor. Taken together, the PacBio Sequel IIe provides PIs with 8x the amount of data at an overall 50% cost reduction. Acquisition of a Sequel IIe would allow PIs to cost-effectively interrogate repetitive regions of viruses, size discrimination of genomic regions to identify repeats correlated to disease, direct detection of recombination events and deletion, splicing and phasing, and more. Given the requested Sequel IIe would be the only instrument catering to the needs of academic researchers in NC, this instrument would not only benefit NIH- funded PIs in Duke’s School of Medicine, but across all of Duke and North Carolina.

公爵测序和基因组技术共享资源（SGT）在生物医学领域为PI服务 来自杜克大学医学院和杜克大学。中士是一个〜6600平方英尺的最先进的设施 拥有超过310万美元的仪器，并拥有十名全职员工。十多年来， 中士始终提供了最新的最先进的基因组服务。像大多数实验室一样，短阅读 DNA/RNA测序是SGT中测序技术的主要形式，因为它非常准确 和成本效益。但是，简读测序无法访问复杂的基因组学区域。完成 短阅读测序方法，包括SGT在内的实验室使用长阅读测序技术。因此， 中士为PI提供了高度准确的短灯光测序读数和中等准确的超长 牛津纳米孔测序读取。但是，中士目前没有提供高度准确的长时间 PACBIO续集IIE系统产生的测序读取。续集IIE，在此要求的乐器 提案将是杜克大学和北卡罗来纳州学术机构的独特资源。 pacbio 最近克服了长期困扰长阅读技术的弱点。这些弱点是 不准确，低通量产生和高成本。 PACBIO续集IIE测序的第一个HIFI库 高度准确。研究人员和PACBIO开发的下一个多种技术增加了吞吐量 长阅读测序。此外，续集IIE具有局部计算能力，可降低人工的劳动。拍摄 PACBIO续集IIE一起为PI提供了8倍的数据量，总成本降低了50％。 获取续集IIE将允许PI经济有效地询问病毒的重复区域，大小 歧视基因组区域以识别与疾病相关的重复，直接检测重组 事件和删除，拼接和估计等等。鉴于请求的续集IIE将是唯一的 满足NC学术研究人员需求的仪器，该工具不仅会受益于NIH- 在杜克大学医学院的PIS资助，但遍布杜克大学和北卡罗来纳州。