CASE REPORTS OF MICROSPORIDIOSIS

微孢子虫病病例报告

• 批准号: 7562334
• 负责人: Elizabeth Schmidt Didier
• 金额: $ 3.04万
• 依托单位: TULANE UNIVERSITY OF LOUISIANA
• 依托单位国家: 美国
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-05-01 至 2008-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7562334
• 关键词: Adolescent; Adult; Affect; Animals; Bilateral; Case Study; Cessation of life; Chiroptera; Computer Retrieval of Information on Scientific Projects Database; Condition; Diagnosis; Electron Microscopy; Encephalitozoon cuniculi; Encephalitozoon hellem; Encephalitozoonosis; Enzyme-Linked Immunosorbent Assay; Female; Fibrosis; Fruit; Funding; Genitourinary system; Genotype; Gossypium; Grant; Histologic; Histopathology; Human; Individual; Infection; Inflammation; Institution; Kyphosis deformity of spine; Lesion; Liver; Maintenance; Microsporidia; Microsporidiosis; Morbidity - disease rate; Neonatal; North America; Organism; Parasites; Pleistophora; Polymerase Chain Reaction; Recombinant DNA; Reporting; Reproduction spores; Research; Research Personnel; Resources; Ribosomal DNA; Rousettus; Saguinus; Salamander; Siblings; Source; United States National Institutes of Health; Vertical Disease Transmission; Western Blotting; day; mortality

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Three species of microsporidia were identified as causes of morbidity and mortality in fruit bats, salamanders, and tamarins that were reported in three sets of case reports. Their relevance was that these microsporidia species also infect humans so these infected animals may serve as reservoirs of infection. Disseminated microsporidiosis was diagnosed in an adult female Egyptian fruit bat (Rousettus aegyptiacus) that died unexpectedly in a zoo. Gross findings included poor body condition, bilateral renomegaly, and mottling of the liver. Histopathological lesions consisted of inflammation associated with intracytoplasmic microsporidian spores in the urogenital tract and liver. PCR established the microsporidian as Encephalitozoon hellem. This is the first documented case of E. hellem in a non-human mammalian species. Captive refugia for four species of plethodontid salamanders were established. Following this successful maintenance, two of the species presented with kyphosis. In one of these species (San Marcos salamander, Eurycea nana), microsporidial organisms within the epaxial musculature were identified in post-mortem histopathology and confirmed by polymerase chain reaction rDNA sequence as most closely similar to Encephalitozoon hellem. However, ultrastructural analysis and further rDNA sequencing suggested a new species of Pleistophora. In these affected individuals, the microsporidial infection produced kyphosis resulting from inflammation and fibrosis of the expaxial musculature to the parasite. Disseminated encephalitozoonosis was diagnosed in 2 sibling, juvenile cotton-top tamarins (Saguinus oedipus) and 3 sibling, neonatal, emperor tamarins (S. imperator) that were captive born in zoos in North America. Histologic examination, histochemical analysis, electron microscopy, and PCR were used to identify the cause as Encephalitozoon cuniculi genotype III. The dam, but not sire, of the emperor tamarins was seropositive for E. cuniculi by ELISA and Western blot immunodetection. The death of two less than one-day-old emperor tamarins from a seropositive dam supports the likelihood of vertical transmission in the tamarins.

该子项目是利用该技术的众多研究子项目之一 资源由 NIH/NCRR 资助的中心拨款提供。子项目及 研究者 (PI) 可能已从 NIH 的另一个来源获得主要资金， 因此可以在其他 CRISP 条目中表示。列出的机构是 对于中心来说，它不一定是研究者的机构。 三种微孢子虫被确定为导致果蝠、蝾螈和狨猴发病和死亡的原因，这在三组病例报告中都有报道。 它们的相关性在于，这些微孢子虫物种也感染人类，因此这些受感染的动物可能成为感染宿主。 一只在动物园意外死亡的成年雌性埃及果蝠（Rousettus aegyptiacus）被诊断出患有播散性微孢子虫病。 总体检查结果包括身体状况不佳、双侧肾肿大和肝脏出现斑点。组织病理学病变包括与泌尿生殖道和肝脏中胞浆内微孢子虫孢子相关的炎症。 PCR 确定该微孢子虫为 Helem 脑炎孢子虫。 这是第一个记录在案的非人类哺乳动物物种中的 E. hellem 病例。 为四种无齿蝾螈建立了圈养保护区。 在这次成功的维护之后，其中两个物种出现了脊柱后凸。 在其中一个物种（圣马科斯蝾螈，Eurycea nana）中，在死后组织病理学中鉴定出了轴外肌肉组织内的微孢子生物，并通过聚合酶链反应 rDNA 序列证实与脑炎蝾螈最相似。 然而，超微结构分析和进一步的 rDNA 测序表明，Pleistophora 是一个新物种。 在这些受影响的个体中，微孢子虫感染由于寄生虫的外轴肌肉组织的炎症和纤维化而产生驼背。 北美动物园圈养出生的 2 只同胞幼年棉顶狨猴 (Saguinus oedipus) 和 3 只同胞新生皇狨猴 (S. imperator) 被诊断为播散性脑炎动物病。 通过组织学检查、组织化学分析、电子显微镜和 PCR 鉴定病因为兔脑炎原虫基因型 III。 通过 ELISA 和蛋白质印迹免疫检测，帝狨猴的母亲（而非父亲）的 E. cuniculi 血清呈阳性。 两只不到一天大的帝狨猴因血清呈阳性的水坝而死亡，这支持了狨猴中垂直传播的可能性。