GASTROINTESTINAL DISEASE IN CAPTIVE RHESUS MACAQUES

圈养恒河猴的胃肠道疾病

• 批准号: 8358108
• 负责人: Elizabeth Schmidt Didier
• 金额: $ 3.72万
• 依托单位: TULANE UNIVERSITY OF LOUISIANA
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-05-01 至 2012-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8358108
• 关键词: Age; Amino Acid Motifs; Animal Hospitals; Animal Housing; Animals; Antibodies; Binding; Calicivirus; Clinic; Cross-Sectional Studies; Diagnostic; Diarrhea; Enteral; Enterocytozoon bieneusi; Feces; Funding; Gastrointestinal Diseases; Gender; Genetic; Grant; High Prevalence; Human; Macaca; Macaca mulatta; Microsporidia; Morbidity - disease rate; National Center for Research Resources; Norovirus; Phylogenetic Analysis; Prevalence; Primates; Principal Investigator; RNA-Directed RNA Polymerase; Reporting; Research; Research Infrastructure; Resources; Sampling; Sapovirus; Serum; Source; Specimen; Testing; United States National Institutes of Health; Virus; cost; neutralizing antibody; nonhuman primate; transmission process

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. Persistent diarrhea is one of the leading causes of morbidity in captive nonhuman primates (NHPs). During the current reporting period, a cross-sectional study was continued to test fecal specimens obtained from rhesus macaques admitted to the veterinary clinics for diarrhea and/or wounding as well as from animals housed in field cages at the Tulane National Primate Research Center (TNPRC) for microsporidia shedding using PCR diagnostics. Of 194 clinic specimens analyzed, 28 of 95 (29.5%) were positive for microsporidia and of 99 field specimens analyzed, 35 of 99 (35.4%) were positive for microsporidia with an overall prevalence of 32.5%. Fisher's Exact test indicated no significant differences in prevalence associated with gender, age, or clinic vs field animals. Enterocytozoon bieneusi was by far, the most common species identified (54 of 64; 84.3 %). The objective of this study is also to determine the prevalence of anti-norovirus (NoV), sapovirus (SaV) and Tulane calicivirus (TV) antibodies in rhesus macaques of the TNPRC and evaluate the antigenic relationship between these viruses. So far, we tested 515 rhesus macaques for this purpose. A high prevalence of NoV (51-61%) and SaV (50-56%) binding antibodies and TV (69%) neutralizing antibodies were detected. The high prevalence of human and rhesus CV-specific serum antibodies suggests the frequent exposure of colony macaques to enteric CVs including the possibility of CV transmission between human and NHP hosts. More recently, 325 randomly selected stool samples collected from TNPRC rhesus macaques were tested using modified degenerate primers targeting conserved amino acid motifs in the calicivirus RNA dependent RNA polymerases (RdRp). Thirty-six (11%) of the 325 stools samples tested yielded TV specific sequences, confirming that TVs are endemic in the TNPRC. According to phylogenetic analysis, the 36 TV isolates can be classified into two genogroups and at least four genetic types.

该子项目是利用资源的众多研究子项目之一 由 NIH/NCRR 资助的中心拨款提供。子项目的主要支持 并且子项目的主要研究者可能是由其他来源提供的， 包括其他 NIH 来源。 子项目可能列出的总成本 代表子项目使用的中心基础设施的估计数量， NCRR 赠款不直接向子项目或子项目工作人员提供资金。 持续性腹泻是圈养非人灵长类动物 (NHP) 发病的主要原因之一。 在本报告所述期间，继续进行一项横断面研究，测试从因腹泻和/或受伤而进入兽医诊所的恒河猴以及杜兰国家灵长类研究中心（TNPRC）野外笼养动物中获得的粪便样本。 ）使用 PCR 诊断进行微孢子虫脱落。 在分析的 194 份临床标本中，95 份中的 28 份（29.5%）呈微孢子虫阳性；在分析的 99 份现场标本中，99 份中的 35 份（35.4%）呈微孢子虫阳性，总体患病率为 32.5%。 费舍尔精确检验表明，与性别、年龄或临床动物相比，患病率没有显着差异。 迄今为止，比氏肠细胞虫是最常见的物种（64 种中的 54 种；84.3%）。 本研究的目的还在于确定 TNPRC 恒河猴中抗诺如病毒 (NoV)、沙波病毒 (SaV) 和杜兰杯状病毒 (TV) 抗体的流行情况，并评估这些病毒之间的抗原关系。到目前为止，我们为此测试了 515 只恒河猴。检测到 NoV (51-61%) 和 SaV (50-56%) 结合抗体以及 TV (69%) 中和抗体的发生率很高。人类和恒河猴 CV 特异性血清抗体的高流行表明群体猕猴经常接触肠道 CV，包括人类和 NHP 宿主之间 CV 传播的可能性。 最近，使用针对杯状病毒 RNA 依赖性 RNA 聚合酶 (RdRp) 中保守氨基酸基序的改良简并引物对从 TNPRC 恒河猴收集的 325 个随机选择的粪便样本进行了测试。测试的 325 个粪便样本中有 36 个 (11%) 产生了 TV 特定序列，证实 TV 在 TNPRC 中流行。根据系统发育分析，36个电视分离株可分为两个基因组和至少四种遗传类型。