PATHOGEN DETECTION AND QUANTIFICATION CORE

病原体检测和定量核心

• 批准号: 8172949
• 负责人: Elizabeth Schmidt Didier
• 金额: $ 6.18万
• 依托单位: TULANE UNIVERSITY OF LOUISIANA
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-05-01 至 2011-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8172949
• 关键词: Antibodies; Computer Retrieval of Information on Scientific Projects Database; Diagnostic; Enzyme-Linked Immunosorbent Assay; Funding; Grant; Immunoassay; Institution; Laboratories; Pathogen detection; Proviruses; Reporting; Research; Research Personnel; Resources; Rivers; SIV; Source; Specific Pathogen Frees; Specimen; Testing; Tuberculosis; United States National Institutes of Health; Virus; Western Blotting; animal colony; germ free condition; instrumentation; nonhuman primate; pathogen; synthetic polymer Bioplex

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The pathogen detection and quantification core (PDQC) was established in 2003 with the purpose of testing for specific pathogens to establish and maintain specific pathogen-free nonhuman primate animal colonies at the TNPRC. The PDQC is comprised of two units. The serodiagnostics unit provides testing for antibodies specific to SIV, STLV, SRV, and Herpes B using ELISA, Western blot immunodetection, and most recently, via Multiplexed Flurometric ImmunoAssay (MFIA; Charles River) using Bioplex instrumentation. This unit also provides diagnostics for tuberculosis via Primagam and Statpak testing. The realtime PCR unit currently functions to detect SRV (virus and provirus). Reliability of testing by both units was corroborated during a three-month period of parallel testing with Charles Rivers Laboratory. During the first 6 months of this reporting period, 1938 specimens were submitted and tested for SRV in the realtime PCR unit. During this same period, the following numbers of specimens were tested in the serodiagnostics unit; 1850 by MFIA, 139 SIV ELISAs, 711 Herpes B ELISAs, 752 STLV ELISAs, 6 STLV western blots, 161 PrimaTB STATPAKs, and 199 Primagam TB tests.

该子项目是利用该技术的众多研究子项目之一 资源由 NIH/NCRR 资助的中心拨款提供。子项目及 研究者 (PI) 可能已从 NIH 的另一个来源获得主要资金， 因此可以在其他 CRISP 条目中表示。列出的机构是 对于中心来说，它不一定是研究者的机构。 病原体检测和定量核心 (PDQC) 成立于 2003 年，目的是检测特定病原体，以在 TNPRC 建立和维持无特定病原体的非人灵长类动物群体。 PDQC 由两个单元组成。 血清诊断单元使用 ELISA、蛋白质印迹免疫检测以及最近使用 Bioplex 仪器通过多重荧光免疫分析（MFIA；Charles River）对 SIV、STLV、SRV 和疱疹 B 特异性抗体进行测试。 该装置还通过 Primagam 和 Statpak 测试提供结核病诊断。 实时PCR装置目前用于检测SRV（病毒和原病毒）。 两个单位测试的可靠性在与查尔斯·里弗斯实验室进行的为期三个月的平行测试中得到了证实。 在本报告期的前 6 个月内，共提交了 1938 个样本并在实时 PCR 装置中进行了 SRV 检测。 同一时期，血清诊断部门对以下数量的标本进行了检测； 1850 次 MFIA、139 次 SIV ELISA、711 次疱疹 B ELISA、752 次 STLV ELISA、6 次 STLV 蛋白质印迹、161 次 PrimaTB STATPAK 和 199 次 Primagam TB 测试。