Microfabrication and Microfluidics Core D

微加工和微流体核心 D

基本信息

批准号：
8461773
负责人：
Susan M Lunte
金额：
$ 21.58万
依托单位：
UNIVERSITY OF KANSAS LAWRENCE
依托单位国家：
美国
项目类别：
财政年份：
资助国家：
美国
起止时间：
至
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/8461773
关键词：
Animal Model Behavior Biochemistry Biological Assay Biological Process Caenorhabditis elegans Cardiovascular Diseases Cells Centers of Research Excellence Chemicals Computers Development Device or Instrument Development Devices Disease Pathway Education and Outreach Embryo Erythrocytes Genetic Genetic Models Growth Human Resources Image Investigation Laboratories Lung diseases Malignant Neoplasms Measurement Measures Methods Microfabrication Microfluidic Microchips Microfluidics Modification Molecular Analysis Molecular Probes Organism Production Proteins Research Infrastructure Research Personnel Resources Slice System Technology Tissues Zebrafish base biological systems design fluorescence imaging in vivo micro-total analysis system microchip nervous system disorder neurotransmitter release novel particle small molecule submicron

Animal Model Behavior Biochemistry Biological Assay Biological Process Caenorhabditis elegans Cardiovascular Diseases Cells Centers of Research Excellence Chemicals Computers Development Device or Instrument Development Devices Disease Pathway Education and Outreach Embryo Erythrocytes Genetic Genetic Models Growth Human Resources Image Investigation Laboratories Lung diseases Malignant Neoplasms Measurement Measures Methods Microfabrication Microfluidic Microchips Microfluidics Modification Molecular Analysis Molecular Probes Organism Production Proteins Research Infrastructure Research Personnel Resources Slice System Technology Tissues Zebrafish base biological systems design fluorescence imaging in vivo micro-total analysis system microchip nervous system disorder neurotransmitter release novel particle small molecule submicron

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项目摘要

Over the past twenty years there has been a tremendous growth in the application of microfabrication technologies that are used to produce computer chips towards the development of microfluidic devices that can be used to study biological systems. Microchip based fluidic systems have been used to analyze the contents of single cells, measure the effect of channel size on ATP release from red blood cells as well as the study of the behavior and growth of C. elegans. The Microfabrication and Microfluidics Core (MMC) will provide the resources and personnel for the production of microfabricated devices that can be used by project investigators for their studies. It will also assist in the development of bioanalytical assays for small molecules and proteins. For the projects described in this COBRE proposal, the initial emphasis will be on the development of devices for trapping and imaging C. elegans, and manipulating zebrafish embryos. The devices developed in this core laboratory will make it possible for project investigators to explore the effect of genetic modifications of model organisms by fluorescence imaging in conjunction with the Genetics and Model Organism Core and the Synthetic Molecular Probes for in vivo Imaging core. In addition, microfluidics systems for maintaining tissue slices and cells will be developed and used for measurement of the release of neurotransmitters and other biomolecules. Lastly, basic fabrication methods will be available for the production of micron and submicron particles, channels and reservoirs for biophysical studies related to the molecular analysis of disease pathways.

在过去的二十年中，微结构技术的应用有了巨大的增长，这些技术用于生产计算机芯片以开发微流体设备，可用于研究生物系统。基于微芯片的流体系统已用于分析单个细胞的含量，测量通道大小从红细胞释放ATP的影响以及对秀丽隐杆线虫的行为和生长的研究。微加工和微流体核心（MMC）将为生产微型制造设备的资源和人员提供资源和人员，这些设备可以由项目研究人员用于其研究。它还将有助于开发小分子和蛋白质的生物分析测定。对于此圆锥形提案中描述的项目，最初的重点将是开发用于捕获和成像秀丽隐杆线虫的设备，并操纵斑马鱼胚胎。该核心实验室中开发的设备将使项目研究人员通过荧光成像与遗传学和模型生物核心以及体内成像核心的合成分子探针结合探索模型生物的遗传修饰的影响。此外，将开发用于维持组织切片和细胞的微流体系统，用于测量神经递质和其他生物分子的释放。最后，将用于与疾病途径分子分析有关的生物物理研究的微米和亚微米，通道和储层的基本制造方法。