Insecticide Resistance in Anopheles minimus Mosquitoes

微小按蚊的杀虫剂抗药性

基本信息

批准号：
8212246
负责人：
Guiyun Yan
金额：
$ 5.88万
依托单位：
UNIVERSITY OF CALIFORNIA-IRVINE
依托单位国家：
美国
项目类别：
财政年份：
2009
资助国家：
美国
起止时间：
2009-12-01 至 2013-11-30
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/8212246
关键词：
Accounting Address Adult Advisory Committees Africa South of the Sahara African Anopheles Genus Anopheles gambiae Anti-malarial drug resistance Asia Bacillus thuringiensis Beds Biological Biological Assay Breeding Case Study China Chloroquine Climate Clinical Cohort Studies Communities Culicidae Data Development Diagnosis Doctor of Philosophy Drug Metabolic Detoxication Ecology Entomology Epidemic Epidemiology Failure Female Foundations Future Genes Genetic Polymorphism Grant Habitats Incidence Infection Insecta Insecticide Resistance Insecticides Kenya Laboratories Larva Life Linear Models Malaria Medical Medical Entomology Metabolic Methods Mixed Function Oxygenases Modeling Molecular Monitor Mosquito Control Mutation Nucleotides Parasites Parents Phenotype Plasmodium falciparum Play Population Population Genetics Postdoctoral Fellow Public Health Publishing Pyrimethamine-Sulfadoxine Regression Analysis Research Research Training Residual state Resistance Role Scientist Seasons Sentinel Site Southeastern Asia Spatial Distribution Statistical Models Students System Temperature Testing Training United States National Institutes of Health Universities World Health Organization base cost effective design land cover land use larval control meter parent grant predictive modeling public health relevance pyrethroid research study resistance mechanism resistance mutation skills training tool transmission process vector vector control vector mosquito

项目摘要

DESCRIPTION (provided by applicant): Malaria is a major public health problem in China. Pyrethroid-impregnated bed nets and indoor spray with pyrethroids are the main malaria vector control tools. However, the wide use of pyrethroids has resulted in the emergence and spread of resistance in mosquito populations. Insecticide resistance has significantly hindered the efforts of malaria control in China and worldwide. This application will focus on resistance mechanisms in an important malaria vector in Southern China, Anopheles minimus C. The central hypothesis is that both knockdown resistance (kdr) and metabolic detoxification confer resistance to pyrethroids in An. minimus, and consequently, the resistance level of a mosquito to pyrethroids is contingent on mutations in kdr gene and monooxygenase activity. The kdr gene polymorphism in natural populations will be determined, and correlations between the kdr mutations, monooxygenase activity with pyrethroid resistance phenotype will be examined. This research will play an important foundation for the development of molecular- or biochemically-based resistance diagnosis tools in this important malaria vector species in China and Southeast Asia. The proposed research will contribute to building research capacity in China by training one Ph.D. student and one postdoc fellow and strengthen the molecular entomology laboratory at the Nanjing Medical University. This research will be conducted as an extension of NIH grant R01 AI050243-07. PUBLIC HEALTH RELEVANCE: This application focuses on resistance mechanisms in the most important malaria vector in Southern China, Anopheles minimus C. Understanding the resistance mechanisms will play an important foundation for the development of new resistance diagnosis tools. Further, this research will contribute to building research capacity in medical entomology in China by providing research training for one Ph.D. student and one postdoc fellow, and strengthen the molecular entomology laboratory in Nanjing Medical University.

描述（由申请人提供）：疟疾是中国的主要公共卫生问题。拟除虫菊酯浸渍的床网和室内喷雾剂带有拟除虫菊酯是主要的疟疾载体控制工具。然而，广泛使用拟除虫菊酯，导致蚊子种群中抗性的出现和扩散。杀虫剂的耐药性极大地阻碍了中国和世界范围内疟疾控制的努力。该应用将集中于中国南部重要的疟疾载体中的抗性机制，Anopheles MinimusC。中心假设是敲低抗性（KDR）和代谢排毒赋予对拟除虫菊酯的耐药性。最小值，因此，蚊子对拟除虫菊酯的电阻水平取决于KDR基因和单氧酶活性的突变。将确定自然群体中的KDR基因多态性，并将检查KDR突变，KDR突变，单氧酶活性与拟除虫菊酯抗性表型的相关性。这项研究将为在中国和东南亚这种重要的疟疾载体物种中开发分子或生化的抗抗性诊断工具的发展奠定重要基础。拟议的研究将通过培训一位博士学位来促进中国建立研究能力。学生和一名博士后研究员，并加强了南京医科大学的分子昆虫学实验室。这项研究将作为NIH授予R01 AI050243-07的扩展。公共卫生相关性：该应用集中在中国南部最重要的疟疾载体中的抵抗机制，Anopheles MinimusC。了解这种阻力机制将为开发新的抗药性诊断工具奠定重要基础。此外，这项研究将通过为一家博士提供研究培训来有助于建立中国医学昆虫学研究能力。学生和一名博士后研究员，并加强了南京医科大学的分子昆虫学实验室。