TRANSGENIC PRAIRIE VOLES TO DISSECT GENETICS/NEURAL CIRCUITRY OF SOCIAL BONDING

转基因草原田鼠解析社会联系的遗传学/神经回路

• 批准号: 8172474
• 负责人: Larry J Young
• 金额: $ 5.48万
• 依托单位: EMORY UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-05-01 至 2011-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8172474
• 关键词: Arts; Computer Retrieval of Information on Scientific Projects Database; DNA; Funding; Gene Expression; Genetic; Goals; Grant; Institution; Lentivirus Vector; Microtus; Mutant Strains Mice; Neurons; Oxytocin; Oxytocin Receptor; Pair Bond; Population; Production; Research; Research Personnel; Resource Development; Resources; Small Interfering RNA; Source; System; Technology; Transgenic Organisms; United States National Institutes of Health; Viral Vector; cell growth regulation; cell type; neural circuit; new technology; prairie vole; relating to nervous system; social attachment; success

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The objectives of this project are to develop transgenic technologies for the selective activation and inactivation of the neural systems regulating pair bonding on the genetic and cellular level. Development of these resources will have two main foci: 1) the genetic inactivation of oxytocin receptor (Oxtr) and 2) the cellular regulation of oxytocin (OT) release within transgenic lines of prairie voles. Toward the first goal we will take advantage of Cre-lox and siRNA technologies that have been used so successfully in mutant mice to regulate gene expression in a temporally regulated and cell-type specific manner. This new technology is made possible by our recent success in creating transgenic voles using viral vector technology. The second approach uses state-of-the-art transgenic optogenetics to precisely regulate the activity of specific neuronal populations using localized photostimulation. We have successfully obtained all of the DNA constructs and begun to construct the lentiviral vectors that we will use in the production of transgenic voles.

该副本是利用众多研究子项目之一 由NIH/NCRR资助的中心赠款提供的资源。子弹和 调查员（PI）可能已经从其他NIH来源获得了主要资金， 因此可以在其他清晰的条目中代表。列出的机构是 对于中心，这不一定是调查员的机构。 该项目的目标是开发转基因技术，以选择性激活和灭活神经系统，从而调节遗传和细胞水平上的配对键合。这些资源的开发将具有两个主要焦点：1）催产素受体（OXTR）的遗传失活和2）在草原田鼠的转基因线中，催产素（OT）释放的细胞调节。 为了实现第一个目标，我们将利用在突变小鼠中如此成功使用的Cre-Lox和siRNA技术，以时间调节和细胞类型的特定方式调节基因表达。我们最近在使用病毒媒介技术创建转基因田鼠方面的成功使这项新技术成为可能。第二种方法使用最先进的转基因光遗传学来精确地调节特定神经元种群的活性，并使用局部光刺激。 我们已经成功获得了所有DNA构建体，并开始构建我们将在转基因田鼠生产中使用的慢病毒载体。