LSU VETERINARY COBRE: MOLECULAR IMMUNOPATHOLOGY CORE

路易斯安那州立大学兽医 COBRE：分子免疫病理学核心

基本信息

批准号：
8167883
负责人：
Konstantin G Kousoulas
金额：
$ 44.3万
依托单位：
LOUISIANA STATE UNIV A&M COL BATON ROUGE
依托单位国家：
美国
项目类别：
财政年份：
2010
资助国家：
美国
起止时间：
2010-05-01 至 2011-04-30
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/8167883
关键词：
Animal Model Antibody Formation Arts Biological Assay Biometry Cell Separation Cells Centers of Research Excellence Clinical Communicable Diseases Computer Retrieval of Information on Scientific Projects Database Confocal Microscopy Consultations Custom DNA Sequence Diagnosis Ensure Funding Future Grant Histocytochemistry Immune In Situ Hybridization In Vitro Institution Laboratories Lasers Louisiana Lymphokines Microscopy Molecular Molecular Biology Nature Pathologist Pathology Production Recombinant DNA Research Research Personnel Resources Services Source Technology Technology Transfer Time Tissues Training Transmission Electron Microscopy United States National Institutes of Health Universities Viral Work base cDNA Library Construction immunopathology in vivo molecular pathology new technology nonhuman primate pathogen protein profiling synthetic polymer Bioplex

项目摘要

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The Louisiana State University COBRE Molecular Immunopathology (MIP) Core is a consortium arrangement of five existing Centralized Facilities and Service Units of the LSU-SVM working together to provide state-of-the art service, as well as to develop new technologies to detect, diagnose and analyze the effect of infectious disease pathogens in vitro and in vivo. Dr. Xavier Alvarez (TNPRC) and Dr. David Scollard (NHDP) will participate in the MIP Core. Their participation ensures technology transfer of state-of-the art confocal and molecular pathological technologies already implemented for non-human primate and other animal models to researchers at LSU-SVM and the COBRE-CEIDR. The MIP Consortium is composed of the following existing SVM Centralized Facilities: 1) BIOMMED concentrates on molecular biology and currently provides advanced recombinant DNA and cDNA library constructions, real-time PCR, DNA sequencing, BioPlex lymphokine profiling, protein production and purification, custom antibody production etc. 2) The Centralized Pathology Service Laboratory currently provides routine anatomical, clinical and molecular pathology, the later including immuno-histochemical approaches to detect pathogens and specialized cells. A specialized Unit wihin this Service Laboratory has been formed under the direction of Dr. Nobuko Wakamatsu (Board Certified Pathologist). This unit will be an intimate part of the MIP Core and will serve as liason to other pathology services. 3) the FACS Facility provides advanced immunological assays for lymphokine profiling, cell sorting, etc. 4) The Microscopy Center provides, transmission and electron microscopy, confocal microscopy and laser capture microscopy. New assays that will be developed by the MIP Core include the use of in situ hybridization to detect, viral , rickettsial and bacterial pathogens, tissue immunoprofiling to determine via confocal and immuno-histochemistry the nature of infiltrating immune cells within diseased tissues, etc. 5) The Biostatistics Service Unit provides statistical consultation and training to LSU-based researchers. It is expected that MIP will be a strong supporting Core of the LSU-SVM-based COBRE-CEIDR in future years receiving support from LSU-SVM, as well as other state and federal sources.

该子项目是利用该技术的众多研究子项目之一资源由 NIH/NCRR 资助的中心拨款提供。子项目和研究者 (PI) 可能已从 NIH 的另一个来源获得主要资金，因此可以在其他 CRISP 条目中表示。列出的机构是对于中心来说，它不一定是研究者的机构。路易斯安那州立大学 COBRE 分子免疫病理学 (MIP) 核心是由 LSU-SVM 现有的五个集中设施和服务单位组成的联盟，共同提供最先进的服务，并开发新技术来检测、诊断和分析传染病病原体在体外和体内的作用。 Xavier Alvarez 博士 (TNPRC) 和 David Scollard 博士 (NHDP) 将参加 MIP Core。他们的参与确保了已在非人类灵长类动物和其他动物模型中实施的最先进的共聚焦和分子病理技术向 LSU-SVM 和 COBRE-CEIDR 的研究人员进行技术转让。 MIP 联盟由以下现有的 SVM 集中设施组成： 1) BIOMMED 专注于分子生物学，目前提供先进的重组 DNA 和 cDNA 文库构建、实时 PCR、DNA 测序、BioPlex 淋巴因子分析、蛋白质生产和纯化、定制抗体2) 中央病理服务实验室目前提供常规解剖、临床和分子病理学服务，后期还包括检测病原体和特殊细胞的免疫组织化学方法。该服务实验室内已在 Nobuko Wakamatsu 博士（委员会认证病理学家）的指导下成立了一个专门部门。该单元将成为 MIP 核心的重要组成部分，并将充当其他病理服务的联络人。 3) FACS 设施提供先进的免疫学分析，用于淋巴因子分析、细胞分选等。4) 显微镜中心提供透射和电子显微镜、共聚焦显微镜和激光捕获显微镜。 MIP Core 将开发的新检测方法包括使用原位杂交来检测病毒、立克次体和细菌病原体，使用组织免疫分析来通过共聚焦和免疫组织化学确定患病组织内浸润免疫细胞的性质等5。）生物统计服务部门为路易斯安那州立大学的研究人员提供统计咨询和培训。预计 MIP 在未来几年将成为基于 LSU-SVM 的 COBRE-CEIDR 的强大支持核心，并获得 LSU-SVM 以及其他州和联邦来源的支持。