Macque

马克

基本信息

批准号：
8198168
负责人：
Francois J Villinger
金额：
$ 119.41万
依托单位：
EMORY UNIVERSITY
依托单位国家：
美国
项目类别：
财政年份：
2011
资助国家：
美国
起止时间：
2011-07-07 至 2016-06-30
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/8198168
关键词：
AIDS Vaccines Adjuvant Adult Age Agonist Alleles Animal Model Animals Antibody Affinity Antibody Formation Arts Autopsy B-Cell Development B-Lymphocytes Binding Biological Preservation Biopsy Blood Breeding CD4 Positive T Lymphocytes Caring Cells Cellular biology Clinical Clinical Chemistry Communication Control Animal DNA Development Dose Ensure Euthanasia Evaluation Experimental Designs Female Future Gender Goals Granulocyte-Macrophage Colony-Stimulating Factor HIV HIV vaccine Helper-Inducer T-Lymphocyte Hematology Histologic Immune Immune response Immunity Immunization Immunologics In Vitro Instruction Investigation Laboratories Macaca Macaca mulatta Mediating Modeling Monitor Monkeys Mus Organ Pathogenicity Pathologic Performance Play Pre-Clinical Model Primates Process Proteins Research Resources Retroviridae Route SIV SIV Vaccines Saliva Sampling Services Simian T-lymphotropic virus 1 Source Specimen Testing Tissue Sample Toll-like receptors Vaccines Vagina Vaginal Douching Viral Viremia Virus animal care design environmental enrichment for laboratory animals lymph nodes neutralizing antibody nonhuman primate novel plasmid DNA prevent programs protective effect rectal research study response sample collection success targeted delivery tissue processing transmission process vaccine development vaccine efficacy

项目摘要

The long-range goal of this Program Project is to develop vaccine strategies against mucosal SIV (and HIV in the future) transmission, with a special focus on identifying local and systemic mechanisms leading to protecfive B cell development and antibody production. The objectives for the Primate Core are to provide the experimental animals and support services needed to facilitate the efficient completion of the aims outlined in Project 1, 2, 3 and 4. This will include the provision of retrovirus-free adult female rhesus macaques from the Yerkes rhesus macaque breeding colonies; immunizafions using the various immunizafion platforms (Projects 1 and 2) sample collections (for all projects) and repeated low dose vaginal challenges with SIVsmE660. The Primate Core tasks also include daily monitoring ofthe experimental animals; periodic physical examinafions with blood collecfions for immunologic and virologic evaluations and procurement/shipment of samples to the Program Project invesfigators; the performance of CBCs and laboratory analyses as needed; Perform euthanasia with sample collecfion and processing for Projects 3 and 4; and the performance of a basic gross and histologic necropsy evaluations of all experimental animals that die or are sacrificed during the course of this study. Provision of these resources and support services will facilitate the development and testing of novel AIDS vaccine concepts proposed in this Program Project. The Primate Core will play a key role in the development of a vaccine to prevent mucosal transmission and pathogenicity of SIV and HIV and the determination Of correlates of such protection. Vaccine efficacy will be evaluated by its ability to 1) prevent acquisition of infecfion (sterilizing immunity) or 2) significant control of viremia post-challenge and preservation of protective immune responses.

该计划项目的长期目标是开发针对粘膜 SIV（和 HIV）的疫苗策略未来）传播，特别关注确定导致保护性 B 细胞发育和抗体产生。灵长类核心的目标是提供实验动物和支持促进有效完成项目 1、2、3 和 4 中概述的目标所需的服务。这将包括提供来自耶克斯恒河猴的无逆转录病毒的成年雌性恒河猴猕猴繁殖地；使用各种免疫平台进行免疫（项目 1 和 2）样本采集（针对所有项目）以及使用 SIVsmE660 重复低剂量阴道挑战。灵长类动物核心任务还包括对实验动物的日常监测；定期体检用于免疫学和病毒学评估的血液采集以及样本的采购/运输计划项目研究员；根据需要进行 CBC 和实验室分析；履行项目 3 和 4 的安乐死以及样本收集和处理；和基本毛的表现对在实验过程中死亡或处死的所有实验动物进行组织学尸检评估这项研究。提供这些资源和支持服务将促进新型艾滋病的开发和检测本计划项目中提出的疫苗概念。灵长类核心将在发展中发挥关键作用预防 SIV 和 HIV 粘膜传播和致病性的疫苗的研制及其测定这种保护的相关性。疫苗功效将通过其以下能力来评估：1) 防止获得感染（灭菌免疫）或 2) 显着攻击后控制病毒血症并保留保护性免疫反应。