Macque

马克

基本信息

批准号：
8516869
负责人：
Francois J Villinger
金额：
$ 135.62万
依托单位：
EMORY UNIVERSITY
依托单位国家：
美国
项目类别：
财政年份：
2013
资助国家：
美国
起止时间：
2013-07-01 至 2016-06-30
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/8516869
关键词：
AIDS Vaccines Adjuvant Adult Age Agonist Alleles Animal Model Animals Antibodies Antibody Affinity Antibody Formation Antigens Arts Autopsy B-Cell Development B-Lymphocytes Binding Biological Preservation Biopsy Blood Breeding CD4 Positive T Lymphocytes Caring Cells Cellular biology Clinical Clinical Chemistry Collection Communication Control Animal DNA Development Diagnostic Dose Ensure Euthanasia Evaluation Experimental Designs Female Future Gender Goals Granulocyte-Macrophage Colony-Stimulating Factor HIV HIV Infections HIV vaccine Helper-Inducer T-Lymphocyte Hematology Histologic Immune Immune response Immunity Immunization Immunologics In Vitro Infection Infection prevention Influenza Instruction Investigation Laboratories Macaca Macaca mulatta Mediating Modeling Monitor Monkeys Mus Organ Pathogenicity Pathologic Performance Physical Examination Play Pre-Clinical Model Primates Process Proteins Research Research Personnel Resources Retroviridae Route SIV SIV Vaccines Saliva Sampling Services Simian T-lymphotropic virus 1 Source Specimen Testing Tissue Sample Toll-like receptors Vaccines Vagina Vaginal Douching Viral Viremia Virus animal care design environmental enrichment for laboratory animals lymph nodes neutralizing antibody nonhuman primate novel plasmid DNA prevent programs protective effect rectal research study response sample collection success targeted delivery tissue processing transmission process vaccine development vaccine efficacy

项目摘要

The long-range goal of this Program Project is to develop vaccine strategies against mucosal SIV (and HIV in the future) transmission, with a special focus on identifying local and systemic mechanisms leading to protective B cell development and antibody production. The objectives for the Primate Core are to provide the experimental animals and support services needed to facilitate the efficient completion of the aims outlined in Project 1, 2, 3 and 4. This will include the provision of retrovirus-free adult female rhesus macaques from the Yerkes rhesus macaque breeding colonies; immunizations using the various immunization platforms (Projects 1 and 2) sample collections (for all projects) and repeated low dose vaginal challenges with SIVsmE660. The Primate Core tasks also include daily monitoring ofthe experimental animals; periodic physical examinations with blood collections for immunologic and virologic evaluations and procurement/shipment of samples to the Program Project investigators; the performance of CBCs and laboratory analyses as needed; Perform euthanasia with sample collection and processing for Projects 3 and 4; and the performance of a basic gross and histologic necropsy evaluations of all experimental animals that die or are sacrificed during the course of this study. Provision of these resources and support services will facilitate the development and testing of novel AIDS vaccine concepts proposed in this Program Project. The Primate Core will play a key role in the development of a vaccine to prevent mucosal transmission and pathogenicity of SIV and HIV and the determination Of correlates of such protection. Vaccine efficacy will be evaluated by its ability to 1) prevent acquisition of infection (sterilizing immunity) or 2) significant control of viremia post-challenge and preservation of protective immune responses.

该计划项目的远程目标是制定针对粘膜SIV（和将来的艾滋病毒）传播的疫苗策略，特别着重于识别局部和系统机制，从而导致保护性B细胞开发和抗体生产。灵长类动物核心的目标是提供实验动物和支持服务，以促进项目1、2、3和4中概述的目标的有效完成。这将包括提供无逆转录病毒的成年雌性恒河猕猴，这些猕猴是猕猴猕猴繁殖菌落的；使用各种免疫平台（项目1和2）采样收集（用于所有项目）的免疫接种，并重复使用SIVSME660进行的低剂量阴道挑战。灵长类动物的核心任务还包括对实验动物的每日监测；通过收集血液的定期进行身体检查，以进行免疫和病毒学评估，并向计划项目调查人员运送样品的采购/运输；根据需要进行CBC和实验室分析的性能；对项目3和4进行样本收集和处理，进行安乐死；以及在本研究过程中对所有死亡或牺牲的实验动物进行基本的总体和组织学尸检评估的表现。提供这些资源和支持服务将有助于该计划项目中提出的新型艾滋病疫苗概念的开发和测试。灵长类动物核心将在开发疫苗中发挥关键作用，以防止SIV和HIV的粘膜传播和致病性以及确定这种保护的相关性。疫苗功效将通过其能力1）防止感染的能力进行评估（消毒免疫）或2）挑战后对病毒血症的显着控制并保存保护性免疫反应。