ISOTOPE SIGNATURE BASED IDENTIFICATION OF CROSSLINKED PEPTIDES BY MS

通过 MS 基于同位素特征的交联肽鉴定

• 批准号: 8171348
• 负责人: Trisha N. Davis
• 金额: $ 4.56万
• 依托单位: UNIVERSITY OF WASHINGTON
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-09-01 至 2011-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8171348
• 关键词: Area; Biological Process; Computational Technique; Computer Retrieval of Information on Scientific Projects Database; Crosslinker; Funding; Grant; Institution; Isotopes; Knowledge; Mass Spectrum Analysis; Methods; Peptides; Publishing; Research; Research Personnel; Resources; Running; Source; Structure; Techniques; United States National Institutes of Health; base; crosslink; novel; protein complex; protein crosslink; protein protein interaction; protein structure

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Knowledge of protein structures and protein-protein interactions is essential for our understanding of biological processes. Obtaining such knowledge often presents a severe challenge. Recent advances in protein crosslinking and mass spectrometry (MS) have shown significant potential to contribute to this area. Here we are developing a novel method to rapidly and accurately identify crosslinked peptides based on their unique isotope signature when digested in the presence of H218O. This method overcomes the need for specially synthesized crosslinkers and/or multiple MS runs required by other techniques. We will validate our method by analyzing proteins/complexes of known structure. The computational technique Rosetta will be modified to validate crosslinks observed against published atomic structures.

该副本是利用众多研究子项目之一 由NIH/NCRR资助的中心赠款提供的资源。子弹和 调查员（PI）可能已经从其他NIH来源获得了主要资金， 因此，可以在其他清晰的条目中表示。列出的机构是 对于中心，这不一定是调查员的机构。 蛋白质结构和蛋白质 - 蛋白质相互作用的知识对于我们对生物过程的理解至关重要。获得此类知识通常会带来严重的挑战。蛋白质交联和质谱法（MS）的最新进展显示出对这一区域贡献的显着潜力。在这里，我们正在开发一种新颖的方法，可以根据其在H218O的存在下消化时根据其独特的同位素特征来快速准确地识别交联的肽。该方法克服了对其他技术所需的特殊合成的交联器和/或多个MS运行的需求。我们将通过分析已知结构的蛋白质/复合物来验证我们的方法。 Rosetta的计算技术将进行修改，以验证针对已发表的原子结构观察到的交联。