ANALYSIS OF TUBULIN

微管蛋白的分析

• 批准号: 8171249
• 负责人: Trisha N. Davis
• 金额: $ 0.14万
• 依托单位: UNIVERSITY OF WASHINGTON
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-09-01 至 2011-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8171249
• 关键词: Animal Model; Cell Cycle; Cell Death; Cell division; Cells; Chromosome Segregation; Chromosomes; Computer Retrieval of Information on Scientific Projects Database; Congenital Abnormality; Eukaryotic Cell; Funding; Genetic Materials; Grant; Human; Institution; Kinetochores; Microtubule-Organizing Center; Microtubules; Modeling; Motor; Plus End of the Microtubule; Process; Protein Dynamics; Proteins; Research; Research Personnel; Resources; Role; Saccharomyces cerevisiae; Source; Structure; Tubulin; United States National Institutes of Health; Yeasts; design; insight; research study; spindle pole body; tumorigenesis

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Accurate chromosome segregation is required for propagation of all cells. Errors in this process are implicated in oncogenesis, birth defects and cell death. Crucial to proper partitioning of the chromosomes during cell division is the establishment, arrangement and then breakdown of a bipolar spindle. The aim of this grant is to further understand the structure, assembly and dynamics of the proteins that create and control the organization of the spindle in the model organism Saccharomyces cerevisiae. The microtubule organizing center in yeast is the spindle pole body (SPB). The SPB is a dynamic structure that undergoes remodeling in a cell-cycle specific manner. We have identified proteins involved in the remodeling process and will characterize them. We have provided a detailed architectural description of the SPB and will continue our structural analysis. The SPB nucleates and organizes microtubules. We will perform a set of experiments designed to distinguish between two current models for nucleation. Finally, we have found that kinetochores can biorient without being attached to the plus-end of microtubules. New insights into biorientation will be obtained from determining the role of kinetochores, motors and other spindle proteins in the formation of the bipolar spindle when plus-end attachment is not involved. Because many proteins and spindle features are conserved from yeast to human, our studies will inform models of how eukaryotic cells correctly distribute their genetic material.

该副本是利用众多研究子项目之一 由NIH/NCRR资助的中心赠款提供的资源。子弹和 调查员（PI）可能已经从其他NIH来源获得了主要资金， 因此，可以在其他清晰的条目中表示。列出的机构是 对于中心，这不一定是调查员的机构。 所有细胞的传播都需要准确的染色体分离。 此过程中的错误与肿瘤发生，出生缺陷和细胞死亡有关。 细胞分裂过程中染色体的适当分配至关重要的是双极纺锤体的建立，排列和分解。 这笔赠款的目的是进一步了解蛋白质的结构，组装和动力学，这些蛋白质在模型有机体酿酒酵母中创建和控制主轴的组织。 酵母中的微管组织中心是纺锤体体（SPB）。 SPB是一种动态结构，以细胞周期特定方式进行重塑。 我们已经确定了与重塑过程有关的蛋白质，并将表征它们。我们提供了SPB的详细体系结构描述，并将继续我们的结构分析。 SPB成核和组织微管。我们将执行一组实验，旨在区分两个当前的成核模型。 最后，我们发现动力学可以生化而不会附着在微管的加上末端。 当不涉及加上末端附着时，将通过确定动力学，电动机和其他纺锤体蛋白在形成双极轴形成中的作用来获得对生物学的新见解。由于许多蛋白质和主轴特征都是从酵母到人类的保守，因此我们的研究将为真核细胞如何正确分配其遗传物质的模型。