DYNAMIC SILAC FOR THE STUDY OF PRION PROPAGATION

用于朊病毒传播研究的动态硅酸

• 批准号: 8169814
• 负责人: STANLEY B PRUSINER
• 金额: $ 0.35万
• 依托单位: UNIVERSITY OF CALIFORNIA, SAN FRANCISCO
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-09-12 至 2011-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8169814
• 关键词: Amino Acids; Cell Culture Techniques; Cell Line; Cells; Cessation of life; Collaborations; Computer Retrieval of Information on Scientific Projects Database; Culture Media; Epitopes; Funding; Grant; Infection; Institution; Label; Mammals; Mass Spectrum Analysis; Measures; Nerve Degeneration; Neurons; Prions; Protocols documentation; Research; Research Personnel; Resources; Source; Techniques; United States National Institutes of Health; instrumentation; novel; protein aggregate; protein structure; transmission process

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Prions are infectious protein aggregates which cause neurodegeneration and death in mammals. Cultured neuronal cells have been used in the study of prions. The study of prion propagation in these cell lines is complicated by an inability to differentiate prions supplied to initiate the infection from new cellular prions. Introducing an novel epitope into cellular prion protein has been used previously to identify prion origin. This technique is problematic because pertubations to protein structure may influence prion transmission. Labeled amino acids in the culture medium would be incorporated into newly synthesized prions. The isotopic label in combination with mass spectroscopy would allow us to identify prions produced in the cell culture. Collaboration with the UCSF Mass Spectrometry Facility would give us access to the instrumentation necessary to measure the extent of isotopic incorporation into the prions we have generated. We have had successful collaborations on similar projects. We envision that this project would use the protocols we have established previously.

该副本是利用众多研究子项目之一 由NIH/NCRR资助的中心赠款提供的资源。子弹和 调查员（PI）可能已经从其他NIH来源获得了主要资金， 因此可以在其他清晰的条目中代表。列出的机构是 对于中心，这不一定是调查员的机构。 王室是传染性蛋白质聚集体，引起哺乳动物的神经退行性和死亡。 培养的神经元细胞已用于王室研究。 这些细胞系中的prion传播的研究使得无法区分为引发新细胞蛋白的prions的prions而变得复杂。 以前已使用将新型表位引入细胞prion蛋白中，以鉴定prion虫的起源。该技术是有问题的，因为蛋白质结构的渗透可能会影响prion传播。培养基中标记的氨基酸将被掺入新合成的王室中。 同位素标签与质谱相结合，可以使我们能够鉴定细胞培养中产生的prions。 与UCSF质谱设施的合作将使我们能够访问所需的仪器，以衡量同位素掺入的程度中。 我们在类似项目上成功合作。 我们设想该项目将使用我们之前已经建立的协议。