TURNOVER RATE OF PRP OLIGOMERS IN THE BRAIN

PRP 寡聚物在大脑中的周转率

• 批准号: 8363794
• 负责人: STANLEY B PRUSINER
• 金额: $ 3.32万
• 依托单位: UNIVERSITY OF CALIFORNIA, SAN FRANCISCO
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-06-01 至 2012-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8363794
• 关键词: Brain; Funding; Grant; Infection; Isotope Labeling; Mass Spectrum Analysis; Measures; National Center for Research Resources; Nerve Degeneration; Neurodegenerative Disorders; Nitrogen Isotopes; Nucleic Acids; Pathology; Principal Investigator; Prion Diseases; Prions; Proteome; Research; Research Infrastructure; Resources; Source; Time; United States National Institutes of Health; cost; particle; protein aggregate; protein degradation; protein metabolism

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. The accumulation of protein aggregates is associated with many neurodegenerative diseases. The presence of these protein aggregates is direct evidence that normal protein metabolism is disrupted. Prions are a primary example of this effect, as they are infectious proteinaceous particles. Although strains of prions have been isolated which differ in incubation time and pathology, there is no nucleic acid component of the prion particles. Infection requires only that normal protein turnover is diverted to the formation of new aggregates. Understanding the propagation of prion disease and how these aggregates cause neurodegeneration requires that we investigate changes in protein turnover due to prion infection. Using nitrogen isotope labeling we will measure protein turnover on a proteome-wide scale to assess perturbations in global protein turnover during prion disease.

该副本是利用资源的众多研究子项目之一 由NIH/NCRR资助的中心赠款提供。对该子弹的主要支持 而且，副投影的主要研究员可能是其他来源提供的 包括其他NIH来源。 列出的总费用可能 代表subproject使用的中心基础架构的估计量， NCRR赠款不直接向子弹或副本人员提供的直接资金。 蛋白质聚集体的积累与许多神经退行性疾病有关。 这些蛋白质聚集体的存在是直接证据表明正常蛋白质代谢被破坏。 王室是这种作用的主要例子，因为它们是传染性的蛋白质颗粒。 尽管已经隔离了幼虫的菌株，但在孵育时间和病理上有所不同，但prion颗粒的核酸成分没有。 感染只要求将正常蛋白转移到新聚集体的形成。 了解病毒疾病的传播以及这些聚集体如何引起神经退行性，我们需要研究由于prion感染引起的蛋白质更新的变化。 使用氮同位素标记，我们将在蛋白质组范围内测量蛋白质更新，以评估prion病期间全球蛋白质更新的扰动。