IDENTIFICATION OF LIPIDS ASSOCIATED WITH PRIONS

与朊病毒相关的脂质的鉴定

• 批准号: 8170935
• 负责人: STANLEY B PRUSINER
• 金额: $ 1.91万
• 依托单位: BOSTON UNIVERSITY MEDICAL CAMPUS
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-06-01 至 2011-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8170935
• 关键词: Animals; Ceramides; Computer Retrieval of Information on Scientific Projects Database; Disease; Funding; Gangliosides; Grant; Institution; Lipids; Preparation; Prions; Research; Research Personnel; Resources; Sampling; Source; Staging; Thin Layer Chromatography; United States National Institutes of Health

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. We have developed conditions to purify prions for structural analysis, but these preparations still contain lipids that associate with the prion protein. One of these lipids is a ganglioside. Another class of lipids that associate with the prion protein are ceramides, which were assigned by thin layer chromatography (TLC). At least one additional component was enriched in the prion-associated lipid fraction on the TLC plate, but it could not easily be identified. At the Resource, LC/MS/MS is being used to identify the components of the prion-associated lipid pool. This has resulted in the identification of more than forty lipids, and has enabled comparisons between the samples. Now the investigators have prepared samples from animals with different stages of disease and these are being analyzed.

该副本是利用众多研究子项目之一 由NIH/NCRR资助的中心赠款提供的资源。子弹和 调查员（PI）可能已经从其他NIH来源获得了主要资金， 因此可以在其他清晰的条目中代表。列出的机构是 对于中心，这不一定是调查员的机构。 我们已经开发了净化结构分析的prion的条件，但是这些制剂仍然包含与prion蛋白相关的脂质。这些脂质之一是神经节。与prion蛋白相关的另一类脂质是神经酰胺，这些脂质是由薄层色谱法（TLC）分配的。在TLC板上与Prion相关的脂质分数中至少富含一个额外的成分，但不容易鉴定出来。在资源下，LC/MS/MS用于识别与Prion相关的脂质池的组成部分。这导致鉴定了40多个脂质，并实现了样品之间的比较。现在，研究人员已经准备了来自具有不同疾病阶段的动物的样品，并且正在分析这些样本。