IDENTIFICATION OF LIPIDS ASSOCIATED WITH PRIONS

与朊病毒相关的脂质的鉴定

• 批准号: 8365561
• 负责人: STANLEY B PRUSINER
• 金额: $ 1.08万
• 依托单位: BOSTON UNIVERSITY MEDICAL CAMPUS
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-06-01 至 2012-08-09
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8365561
• 关键词: Animals; Biology; Ceramides; Complex Mixtures; Disease; Funding; Gangliosides; Grant; Lipids; Mass Spectrum Analysis; Medicine; National Center for Research Resources; Preparation; Principal Investigator; Prions; Research; Research Infrastructure; Resources; Sampling; Source; Staging; Thin Layer Chromatography; United States National Institutes of Health; cost; liquid chromatography mass spectrometry; tandem mass spectrometry

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. We have developed conditions to purify prions for structural analysis, but these preparations still contain lipids that associate with the prion protein. Included in the lipid poos are gangliosides and ceramides, which were initially assigned by thin layer chromatography (TLC); complex mixtures of these classes have now been defined by tandem mass spectrometry. At the Resource, LC/MS/MS is being used to identify the components of the prion-associated lipid pool. This has resulted in the identification of more than one hundred lipids, and has enabled comparisons between the samples. Further samples from animals with different stages of disease are being analyzed.

该副本是利用资源的众多研究子项目之一 由NIH/NCRR资助的中心赠款提供。对该子弹的主要支持 而且，副投影的主要研究员可能是其他来源提供的 包括其他NIH来源。 列出的总费用可能 代表subproject使用的中心基础架构的估计量， NCRR赠款不直接向子弹或副本人员提供的直接资金。 我们已经开发了净化结构分析的prion的条件，但是这些制剂仍然包含与prion蛋白相关的脂质。脂质脉中包含的是神经节苷脂和神经酰胺，最初由薄层色谱法（TLC）分配；这些类别的复杂混合物现已通过串联质谱法定定义。在资源下，LC/MS/MS用于识别与Prion相关的脂质池的组成部分。这导致了一百多个脂质的识别，并可以在样品之间进行比较。正在分析来自具有不同疾病阶段的动物的其他样本。