Trace Metals Core

微量金属核心

• 批准号: 8065871
• 负责人: Joseph H Graziano
• 金额: $ 40.39万
• 依托单位: COLUMBIA UNIVERSITY HEALTH SCIENCES
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-04-21 至 2011-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8065871
• 关键词: AA Spectrophotometry; Acids; Arsenic; Bangladesh; Basic Science; Biological; Biological Markers; Biomedical Research; Blood; Blood Donations; Blood specimen; Carbon; Cells; Child; Childhood; Clara cell; Cohort Studies; Coupled; Creatinine; Enzyme-Linked Immunosorbent Assay; Epidemiologic Studies; Exposure to; Grant; Health; High Pressure Liquid Chromatography; Intelligence; Laboratories; Lead; Manganese; Mass Spectrum Analysis; Measurement; Measures; Metabolism; Metal exposure; Metals; Methodology; National Institute of Environmental Health Sciences; Natural graphite; New Hampshire; Oxidative Stress; Performance; Plasma; Proteins; Quality Control; Reaction; Research Personnel; Research Project Grants; Running; Sample Size; Sampling; Serum; Site Visit; Specimen; Structure of nail of toe; Superfund; Technology; Testing; Toxic effect; Trace metal; Urine; analytical method; base; blood lead; cost; drinking water; exposed human population; indium arsenide; member; novel; programs; protein metabolite; tool; urinary

Biomarkers of exposure to metals and metalloids have proven to be powerful tools for epidemiologic studies that seek to study associations between exposures and human health effects. The Trace Metals Core Lab came into existence in 1994 as part of our NIEHS Center, and received a rating of outstanding at NIEHS Center site visits that occurred in 1997 and 2002. The Laboratory participates in multiple quality control programs and has an outstanding record of past performance. Biomedical research Projects #2, #3 and #4, which take place in Bangladesh and New Hampshire, propose to test a set of novel hypotheses concerning the mechanisms and effects of exposure to As and Mn from drinking water. These projects collectively require the analysis of more than 60,000 biological specimens; this estimate approximates the total of 70,000 specimens that have been successfully measured during the past five years. The biological specimens to be analyzed and stored in the Trace Metals Core Lab include blood, urine and toenails. The methodologies employed will include graphite furnace atomic absorption spectrophotometry (GFAAS) (e.g., arsenic in urine and lead in blood), inductively-coupled plasma mass spectrometry with dynamic reaction cell (ICP-MS-DRC) (e.g., total As, Mn and Se in blood), HPLC coupled to ICP-MS-DRC (e.g., arsenic metabolites in urine and blood), and ELISA technology (clara cell protein-16). The Trace Metals Core Laboratory is well equipped to carry out these and other requested analyses at no cost to members of this Superfund Basic Research Program.

金属和类金属暴露的生物标志物已被证明是流行病学研究的有力工具 旨在研究暴露与人类健康影响之间的关联。微量金属核心实验室 于 1994 年作为 NIEHS 中心的一部分成立，并在 NIEHS 中获得优秀评级 1997 年和 2002 年进行的中心实地考察。 实验室参与多项质量控制计划，并在过去拥有出色的记录 表现。生物医学研究项目 #2、#3 和 #4，在孟加拉国和新罕布什尔州进行 汉普郡，提议测试一系列关于暴露于环境的机制和影响的新假设 饮用水中的砷和锰。这些项目总共需要分析 60,000 多个 生物标本；这一估计大约是已成功检测到的 70,000 个样本的总数 过去五年中测量的。 微量金属核心实验室待分析和储存的生物样本包括血液、尿液和 脚趾甲。采用的方法包括石墨炉原子吸收分光光度法 (GFAAS)（例如尿液中的砷和血液中的铅），电感耦合等离子体质谱法 动态反应池 (ICP-MS-DRC)（例如血液中的总砷、锰和硒）、与 ICP-MS-DRC 联用的 HPLC （例如尿液和血液中的砷代谢物）和 ELISA 技术（Clara 细胞蛋白-16）。踪迹 金属核心实验室装备精良，可以免费进行这些分析和其他要求的分析。 该超级基金基础研究计划的成员。