FLOW CYTOMETRY

流式细胞术

基本信息

批准号：
8180977
负责人：
Ellen A Lumpkin
金额：
$ 12.89万
依托单位：
BAYLOR COLLEGE OF MEDICINE
依托单位国家：
美国
项目类别：
财政年份：
2010
资助国家：
美国
起止时间：
2010-09-17 至 2015-06-30
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/8180977
关键词：
Address Arts Cancer Center Cancer Center Support Grant Cell Count Cell Cycle Progression Cell Separation Cells Collaborations Color Communities Consultations Cytometry DNA Data Analyses Ensure Equipment Flow Cytometry Flow Cytometry Shared Resource Fluorescence Fluorescence-Activated Cell Sorting Foundations Funding Future Goals Grant Hour Housing Institution Magnetism Medicine Minnesota Mission Optics Pediatric Hospitals Preparation Protocols documentation Recording of previous events Recruitment Activity Reporter Research Research Personnel Resource Sharing Resources Sampling Seeds Services Site Sorting - Cell Movement Surveys Technology Texas Time Training Transcriptional Regulation Tumor Suppressor Genes United States National Institutes of Health angiogenesis anticancer research base cancer stem cell cancer therapy cell separator college cost cost effective experience improved instrument instrumentation magnetic cell separation meetings member neoplastic cell novel operation programs repaired square foot transforming virus

项目摘要

The goal of the Flow Cytometry Shared Resource to provide users with cost-effective instrumentation, expertise and training for cell sorting and analysis. This technology continues to develop at a rapid pace, especially with the advent of novel fluorescent reporters, increased computational capacity and more cost-effective optical equipment. To meet our members' increasing demands for state-of-the-art flow cytometry, the DLDCC and BCM administration collaborated to create an entirely new flow cytometry facility in 2007. Renovation, operating costs and instrumentation has been supported by $1.7 million in BCM institutional funds and >$600,000 in DLDCC funds. The revamped Facility is housed in newly renovated, centrally located space, which is available to trained users 24 h a day via key-card access. State-of-the-art instrumentation, all of which has been purchased in the last three years, includes two fully loaded florescence-activated cell sorters, three flow analyzers and a magnetic cell separator. The Resource is directed by Dr, Ellen A. Lumpkin, who has over nine years of experience in flow cytometry, and Mr. Joel Sederstrom, who was recruited from the Univ. of Minnesota's Cancer Center Flow Cytometry Core in a national search. To ensure optimal use of services, the Resource provides consultations, training and protocols for sample preparation, flow analysis and cell sorting. The Resource is also staffed with two full-time experienced flow cytometrists who perform operator-assisted cytometry, and assist users with data analysis. With the Resource's improved services and capacity, FACS sorting has increased by >500% and FACS analysis has increased 160% among Cancer Center members. At present, the Resource operates near 100% of its capacity, with 78% of usage occupied by 65 Cancer Center investigators whose membership spans all Scientific Programs. Future plans include further expanding services by recruiting an additional cytometrist and by including a second site at our affiliated institution, Texas Children's Hospital Cancer Center.

流式细胞仪共享资源的目标是为用户提供具有成本效益的仪器，专业知识和细胞分类和分析的培训。这项技术继续以快速的速度发展，尤其是随着新型荧光记者的出现，计算能力提高和更具成本效益的光学设备。为了满足我们成员对最先进的流式细胞仪的不断增长的需求，DLDCC和BCM政府于2007年合作，在2007年创建了全新的流式细胞仪设施。装修，运营成本和工具在BCM Institutition中得到了170万美元的支持资金和60万美元的DLDCC资金。经过改建的设施设有新近装修的中心空间，每天24小时可通过钥匙卡访问提供训练的用户。最先进的仪器，所有这些都有在过去三年中购买，包括两个满载的荧光激活的细胞分类器，三个流动分析仪和一个磁细胞分离器。该资源由艾伦·A·隆普金（Ellen A.明尼苏达州的癌症中心流式细胞仪核心在国家搜索中。为了确保最佳使用服务，资源提供了咨询，培训和协议，用于样本准备，流量分析和细胞分类。该资源还配备了两名专职经验丰富的流式细胞学师，他们执行操作员辅助辅助细胞术，并为用户提供数据分析。随着资源的改善服务和能力，FACS分类增长> 500％，而FACS分析在癌症中心成员中增加了160％。目前，该资源的运营量接近其容量的100％，其中78％的用法占据了65名癌症中心调查人员的占用，他们的会员资格涵盖了所有科学计划。未来的计划包括通过招募额外的细胞计量师以及在我们的分支机构机构，得克萨斯州儿童医院癌症中心招募第二个地点来进一步扩大服务。