BD Biosciences Special Order LSRII

BD Biosciences 特别订单 LSRII

• 批准号: 7591572
• 负责人: Ellen A Lumpkin
• 金额: $ 42.98万
• 依托单位: BAYLOR COLLEGE OF MEDICINE
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-05-01 至 2010-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7591572
• 关键词: AIDS/HIV problem; Aging; Aging-Related Process; Bacteria; Cancer Biology; Cardiovascular system; Cell Separation; Cell surface; Cells; Communities; Congenital Heart Defects; Cyan Fluorescent Protein; Cytometry; Detection; Development; Dyes; Fluorescein; Fluoresceins; Fluorochrome; Funding; Green Fluorescent Proteins; Human; Immunology; Individual; Lasers; Malignant Neoplasms; Medical center; Medicine; Phycoerythrin; Process; Proteins; Regenerative Medicine; Research; Research Personnel; Research Project Grants; Societies; Stroke; Texas; Tube; United States National Institutes of Health; Viola; Yeasts; allophycocyanin; base; college; design; detector; flexibility; fluorophore; instrument; particle; public health relevance; red fluorescent protein; solid state; stem cell therapy

DESCRIPTION (provided by applicant): This application is for funds to purchase a BD Biosciences Special Order LSRII quadruple-laser FACS analyzer that will be situated in the new Cytometry and Cell Sorting Core at Baylor College of Medicine (BCM). This instrument has been selected to serve the purposes of over 16 investigators involved in over 18 NIH- funded projects. This instrument has been designed to serve two key purposes in our community. First, many BCM investigators require the capacity to analyze on multiple fluorescent markers concomitantly with multiple cell surface markers. Our four-laser instrument will allow analysis based on multiple parameters simultaneously such as mCherry, cyan fluorescent protein, yellow fluorescent protein, and allophycocyanin (APC) or sytox red. It will have a 561-nm solid-state laser, allowing detection of red fluorescent protein derivatives such as mCherry and increasing the sensitivity of detecting commonly used probes such as phycoerythrin. It will also be equipped with a 405-nm solid-state laser enabling the use of violet sensitive probes including cyan fluorescent protein and the fluorochrome Pacific Blue. Additionally, a 640-nm laser will allow detection of fluorochromes such as APC and its conjugates as well as the viability dye sytox red, which will give added power and flexibility to all investigators needing multiple easy-to-use parameters for analysis. Finally, the instrument will be equipped with a 488-nm solid-state laser enabling detection of standard fluorophores including green fluorescent protein, yellow fluorescent protein and fluorescein. The second key purpose for this flow analyzer is small-particle detection to study bacteria and yeast, which is critical for four groups of investigators at BCM. Thus, the instrument will include a forward scatter photo-multiplier tube detector enabling small particle detection and analysis, directly enhancing the research using bacteria and yeast. This instrument will afford maximum capabilities as well as maximum flexibility to best serve the evolving needs of the diverse BCM investigators and Texas Medical Center research community. This instrument is essential for the broad base of health-related research at BCM, including projects aimed at developing new therapies in regenerative medicine, cancer, HIV/AIDS, immunology and aging, which will contribute to the overall wellness of our society. Public Health Relevance: Our research at the Baylor College of Medicine requiring a four-laser LSRII flow cytometer will specifically study processes within cells allowing us to better understand stem cell therapies, aging processes, cancer biology and cardiovascular development. The requested LSRII flow cytometer is critical for determining the complexity and differences between individual cells, which is essential for our research projects aimed at developing new treatments or cures for serious human illnesses including cancer, heart abnormalities, stroke and HIV/AIDS.

描述（由申请人提供）：本申请用于购买 BD Biosciences 特别订购 LSRII 四激光 FACS 分析仪的资金，该分析仪将安装在贝勒医学院 (BCM) 的新细胞计数和细胞分选核心中。该仪器已被选择为参与超过 18 个 NIH 资助项目的超过 16 位研究人员提供服务。该工具的设计旨在服务于我们社区的两个关键目的。首先，许多 BCM 研究人员需要能够同时分析多种荧光标记和多种细胞表面标记。我们的四激光仪器将允许同时基于多个参数进行分析，例如 mCherry、青色荧光蛋白、黄色荧光蛋白和别藻蓝蛋白 (APC) 或 sytox 红。它将配备 561 nm 固态激光器，可检测 mCherry 等红色荧光蛋白衍生物，并提高检测藻红蛋白等常用探针的灵敏度。它还将配备 405 nm 固态激光器，支持使用紫光敏感探针，包括青色荧光蛋白和荧光染料太平洋蓝。此外，640 nm 激光将允许检测荧光染料，如 APC 及其缀合物以及活力染料 sytox red，这将为所有需要多个易于使用的参数进行分析的研究人员提供更多的功能和灵活性。最后，该仪器将配备 488 nm 固态激光器，能够检测标准荧光团，包括绿色荧光蛋白、黄色荧光蛋白和荧光素。该流量分析仪的第二个关键用途是通过小颗粒检测来研究细菌和酵母，这对于 BCM 的四个研究小组至关重要。因此，该仪器将包括一个前向散射光电倍增管检测器，可实现小颗粒检测和分析，从而直接加强利用细菌和酵母的研究。该仪器将提供最大的功能和最大的灵活性，以最好地满足不同 BCM 研究人员和德克萨斯医学中心研究界不断变化的需求。该仪器对于 BCM 广泛的健康相关研究基础至关重要，包括旨在开发再生医学、癌症、艾滋病毒/艾滋病、免疫学和衰老方面新疗法的项目，这将有助于我们社会的整体健康。公共健康相关性：我们在贝勒医学院的研究需要四激光 LSRII 流式细胞仪，专门研究细胞内的过程，使我们能够更好地了解干细胞疗法、衰老过程、癌症生物学和心血管发育。所需的 LSRII 流式细胞仪对于确定单个细胞之间的复杂性和差异至关重要，这对于我们旨在开发新疗法或治愈人类严重疾病（包括癌症、心脏异常、中风和艾滋病毒/艾滋病）的新疗法或治愈方法的研究项目至关重要。