Radio Frequency Probe Development

射频探头开发

• 批准号: 8116481
• 负责人: William W Brey
• 金额: $ 14.36万
• 依托单位: FLORIDA STATE UNIVERSITY
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/8116481
• 关键词: Affect; Area; Binding Proteins; Biological; Biological Assay; Cells; Chin; Detection; Development; Dialysis procedure; Frequencies; Health; Heating; Indium; Injection of therapeutic agent; Liquid substance; Medicine; Membrane; Methods; Molecular Conformation; Performance; Preparation; Proteins; Radio; Resolution; Sampling; Solutions; System; Techniques; Technology; Temperature; Testing; Tube; cell fixing; design and construction; electric field; improved; magnetic field; programs; protein structure; research study; solid state; solid state nuclear magnetic resonance

The improvement of RF sample probes is crucial to the development of high field biological solid state NMR. Unlike solution NMR, solid state NMR techniques require exposing the delicate biological sample to very high intensity radio frequency (RF) magnetic fields. Unless the electric field component E of these fields is minimized, the sample will very likely be heated to destruction before the experiment is completed. In Core B, we will apply the "low-E" technology that we have developed for double frequency widebore NMR to the important areas of triple resonance and more accessible standard bore magnets. We will also adapt these probes for the sample/flow applications that are a centerpiece of the proposal. The technology developed in this Core will not only be needed to carry out the scientific program described in the proposal, but it will broadly impact the ability of NMR spectroscopists to conduct protein structure and dynamics studies in the solid state. Any area of health and medicine that benefits from a better understanding of proteins may be affected.

RF样品探针的改善对于高场生物固态NMR的发展至关重要。 与溶液NMR不同，固态NMR技术需要将精致的生物样品暴露于非常 高强度射频（RF）磁场。除非这些场的电场组件E是 最小化，在实验完成之前，很可能将样品加热到破坏。核心 B，我们将应用我们为双频宽孔NMR开发的“低E”技术 三重共振的重要领域和更容易获得的标准孔磁铁。我们还将适应这些 针对该提案的核心的样品/流动应用的探针。 该核心中开发的技术不仅需要进行描述的科学计划 在提案中，但它将广泛影响NMR光谱学家进行蛋白质结构和 固态的动力学研究。任何受益于更好的健康和医学领域 了解蛋白质可能会受到影响。