Solution-Phase Biomolecular NMR at 24 T Fields Using New High Temperature Superco

使用新型高温 Superco 在 24 T 场进行溶液相生物分子 NMR

基本信息

批准号：
8752012
负责人：
William W Brey
金额：
$ 17万
依托单位：
FLORIDA STATE UNIVERSITY
依托单位国家：
美国
项目类别：
财政年份：
2014
资助国家：
美国
起止时间：
2014-08-01 至 2017-04-30
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/8752012
关键词：
Awareness Biomedical Research Biomolecular Nuclear Magnetic Resonance Calculi Cells Communities Community Healthcare Complex Crystallization Data Dependence Development Drug Industry Environmental Wind Face Feedback Filament Future Goals Heating High temperature of physical object Joints Lead Length Location Magnetic Resonance Magnetic Resonance Imaging Magnetic Resonance Spectroscopy Membrane Proteins Nucleic Acids Operating System Pharmacologic Substance Phase Physiological Proteins Radiology Specialty Recording of previous events Research Research Personnel Resolution Resources Rest Role Running Sampling Science Signal Transduction Societies Solutions Spottings Structure System Techniques Technology Temperature Time United States National Institutes of Health base clinical Diagnosis cold temperature density design drug development improved insight instrument magnetic field next generation prevent prototype public health relevance research study screening tool wound

项目摘要

DESCRIPTION (provided by applicant): NMR is central to both the academic and industrial biomedical communities. Further progress in NMR is challenged by ultimate limitations in the low-temperature superconductor (LTS) wires used in NMR/MRI magnets since the 1970s, that prevent them from operating beyond 24T in a persistent, non-driven mode. This proposal builds on a recent NHMFL-based development that has delivered the first coil operating in a stable superconducting state at fields e34 T, and proposes to use this breakthrough for building a fully- superconducting solution NMR system operating above 1 GHz. To achieve this, and to demonstrate the feasibility of exploiting these ideas to carry biomolecular solution-state NMR to this and at higher (H30T) magnetic fields, we aim to: (i) develop a high homogeneity coil of round multifilamentary Bi-2212 wire, a new kind of high-temperature superconductor (HTS) developed by Larbalestier et al in 2012 and that unlike previously proposed HTSs can carry a high current density beyond 30 T; (ii) operate this coil within an existing Oxford Instrument LTS outsert magnet running in persistent mode, to deliver a room-temperature magnetic field sweet spot in excess of 23.5 T over a 10 mm DSV with homogeneities and relevant field instabilities of H1- 2 ppm; (iii) exploit the expertise that over the last decade NHMFL has amassed in field stabilization and probe construction, to demonstrate the feasibility of using such a setup at room temperature with H30 ppb stabilities and homogeneities over 150 ¿L samples; (iv) place this system to the disposition of the NMR community at large as part of NHMFL's facility role, in order to exploit it for paramagnetic, membrane-protein, in cell and protein bioNMR research, as well as to get critical feedback on ways to further pursue this breakthrough.

描述（由适用提供）：NMR对学术和工业生物医学群落都是核心。自1970年代以来，NMR/MRI磁铁中使用的低温超导体（LTS）电线的最终局限性挑战了NMR的进一步进展，这阻止了它们在持久的，非驱动模式下以24T超过24T的运行。该提案建立在最近基于NHMFL的开发的基础上，该开发在Fields E34 T的稳定超导状态下运行了第一个线圈，并提出了使用此突破来构建完全实用的解决方案的NMR系统，而在1 GHz以上运行的NMR系统。 To achieve this, and to demonstrate the feasibility of exploiting these ideas to carry biomolecular solution-state NMR to this and at higher (H30T) magnetic fields, we aim to: (i) develop a high homogeneity coil of round multifilamentary Bi-2212 wire, a new kind of high-temperature superconductor (HTS) developed by Larbalestier et al in 2012 and that unlike previously proposed HTSs can carry a高电流密度超过30吨；（ii）在现有的牛津仪器LTS外部磁铁中运行此线圈，以持久模式运行，以在10 mm DSV上提供超过23.5 t的室温磁场，并具有H1-2 ppm的同质和相关的现场不稳定性；（iii）利用了以下专业知识：在过去的十年中，NHMFL在现场稳定和探测构建中积累了，以证明在室温下使用H30 PPB系统和150次样品的同质性在室温下使用这种设置的可行性；（iv）将该系统作为NHMFL设施角色的整个NMR社区的处置，以便将其利用在细胞和蛋白质BIONMR研究中，并将其用于顺磁性，膜 - 蛋白质研究，并在进一步掩饰这一突破的方法上获得关键的反馈。