National Resource For Advanced NMR Technology

国家先进核磁共振技术资源

• 批准号: 10217173
• 负责人: William W Brey
• 金额: $ 101.35万
• 依托单位: FLORIDA STATE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2017
• 资助国家: 美国
• 起止时间: 2017-08-01 至 2023-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10217173
• 关键词: 2,4-Dinitrophenol; Address; Alzheimer' s Disease; Applied Research; Automobile Driving; Bacterial Drug Resistance; Basic Science; Biological; Biology; Biomedical Research; Biomedical Technology; Chemistry; Communities; Complement; Complex; Complex Mixtures; Cryoelectron Microscopy; Crystallography; Diabetes Mellitus; Disease; Drug Targeting; Educational workshop; Electrons; Funding; Generations; Goals; HIV; High temperature of physical object; Hybrids; Insulin Resistance; International; Kinetics; Lead; Liquid substance; Macromolecular Complexes; Mass Spectrum Analysis; Membrane Proteins; Metabolic; Microbial Biofilms; NMR Spectroscopy; Nuclear; Nuclear Magnetic Resonance; Proteins; Protons; Publications; RF coil; Research; Resolution; Resources; Roentgen Rays; Science; Series; Services; Signal Transduction; Structure; Techniques; Technology; Temperature; Time; Training; Training Activity; Tuberculosis; United States National Institutes of Health; Virus Replication; X-Ray Crystallography; design and construction; drug development; frontier; insight; instrument; instrumentation; interest; kinetic model; lectures; macromolecule; meetings; metabolomics; new technology; news; novel; open data; programs; protein complex; scientific organization; solid state nuclear magnetic resonance; structural biology; tool; web site

National Resource for Advanced NMR Technology OVERALL - Project Summary/Abstract Nuclear magnetic resonance (NMR) spectroscopy is a unique set of experimental tools for understanding the intricacies of biology from macromolecular complexes to complex mixtures, from atomic resolution structure to dynamics on timescales of picoseconds to kiloseconds, from chemistry to functional mechanisms and kinetic rates. No other technology has such breadth and potential for basic and applied research and for interfacing with other technologies, such as X-ray crystallography, small angle X-ray scattering, Cryo-EM, and many other spectroscopic tools. Structural characterization serves as the starting point, providing a framework for understanding biological activities, functional mechanisms and kinetic models that can be added by NMR. Dynamics can be exceptionally well characterized by NMR and this can lead to detailed understanding about how proteins and other macromolecules function, how complexes are formed, and sometimes how kinetic rates are achieved. The solution NMR spectroscopy of complex mixtures has been shown to be particularly useful in combination with mass spectrometry for metabolomics and other complex mixtures. Here, we focus on the frontiers of NMR technology made possible by recent breakthroughs in materials research and instrumentation, and their implementation for a broad user community interested in pursuing fundamental questions at atomic resolution at the forefront of biomedical research. Three TR&Ds advance the sensitivity of NMR each with novel technology – the first through use of high temperature superconductors for RF coils leading to unique sensitivity for solution NMR spectroscopy. TR&D2 takes advantage of a 600 MHz DNP instrument recently installed at the Magnet Lab that will provide enhanced sensitivity through the transfer of magnetization from electrons to protons. New and much more robust DNP probes with an expanded temperature range will be developed. TR&D3 takes advantage of the 36T Series Connected Hybrid for NMR spectroscopy – a jump in field strength of more that 50% equivalent to a jump in field strength from 17T (1990) to 23.5T (2016) that occurred over the past 26 years! This will lead to dramatic enhancements in sensitivity and even more spectacular reductions in signal averaging time. The science will be driven by an excellent team of DBPs and even more C&Ss that span a very broad range of science. A major team effort will be placed on training a new generation of NMR users through an annual pair of workshops and dissemination through publications and presentations at meetings, through a wide variety of scientific organizations and the news media; through a dedicated website for this Resource and through our training activities; as well as posting of our training lectures and video of demonstrations.

国家高级NMR技术资源 总体 - 项目摘要/摘要 核磁共振（NMR）光谱是一组独特的实验工具，用于理解 从大分子复合物到复杂混合物的生物学的复杂性，原子分辨率 从化学到功能机制的Picseconds到千秒的时间尺度的动力学结构 和动力学率。没有其他技术具有基础和应用研究的广度和潜力， 与其他技术的接口，例如X射线晶体学，小角度X射线散射，冷冻EM和 许多其他光谱工具。结构表征是起点，提供了一个框架 为了理解NMR可以添加的生物学活动，功能机制和动力学模型。 NMR的动力学可能是异常很好的特征，这可能会导致对 蛋白质和其他大分子如何发挥作用，如何形成复合物，有时是如何动力学的 达到了费率。复杂混合物的溶液NMR光谱已被证明特别是 与代谢组学和其他复杂混合物的质谱结合使用。在这里，我们集中精力 在NMR技术的前沿，由于材料研究和 仪器及其对有兴趣追求基本的广泛用户社区的实施 在生物医学研究最前沿的原子分辨率的问题。 三个TR＆D提高了NMR对新技术的敏感性 - 首先是使用高 RF线圈的温度超导体导致对溶液NMR光谱的独特灵敏度。 TR＆D2 利用最近在磁铁实验室安装的600 MHz DNP仪器 通过从电子到质子的磁化转移的灵敏度。新的，更强大的DNP 将出现扩大温度范围的问题。 TR＆D3利用36T系列 NMR光谱的连接混合动力 - 野外强度的跳高超过50％等于跳跃 从过去26年中发生的17T（1990）到23.5t（2016）的现场强度！这将导致戏剧性 灵敏度的增强以及信号平均时间的更大减少。科学会 由一支出色的DBP团队以及更多跨越广泛科学的C＆SS驱动。一个 将通过一对年度一对培训新一代NMR用户进行主要团队努力 通过会议上的出版物和演讲进行讲习班和传播 科学组织和新闻媒体；通过此资源的专用网站以及我们的 培训活动；以及我们的培训讲座和演示视频的发布。

项目成果

Probing Interactions of γ-Alumina with Water via Multinuclear Solid-State NMR Spectroscopy.

通过多核固态核磁共振波谱探测 γ-氧化铝与水的相互作用

• DOI: 10.1002/cctc.201901838
• 发表时间: 2020-03-19
• 期刊: ChemCatChem
• 影响因子: 4.5
• 作者: Shen L;Wang Y;Du JH;Chen K;Lin Z;Wen Y;Hung I;Gan Z;Peng L
• 通讯作者: Peng L

Revealing Brønsted Acidic Bridging SiOHAl Groups on Amorphous Silica-Alumina by Ultrahigh Field Solid-State NMR.

• DOI: 10.1021/acs.jpclett.1c02975
• 发表时间: 2021-12-02
• 期刊: JOURNAL OF PHYSICAL CHEMISTRY LETTERS
• 影响因子: 5.7
• 作者: Wang, Zichun;Chen, Kuizhi;Jiang, Yijiao;Trebosc, Julien;Yang, Wenjie;Amoureux, Jean-Paul;Hung, Ivan;Gan, Zhehong;Baiker, Alfons;Lafon, Olivier;Huang, Jun
• 通讯作者: Huang, Jun

Dual Active Sites on Molybdenum/ZSM-5 Catalyst for Methane Dehydroaromatization: Insights from Solid-State NMR Spectroscopy.

用于甲烷脱氢芳构化的钼/ZSM-5 催化剂上的双活性位点：来自固态核磁共振波谱的见解。

• DOI: 10.1002/anie.202017074
• 发表时间: 2021-05-03
• 期刊: Angewandte Chemie (International ed. in English)
• 作者: Gao W;Qi G;Wang Q;Wang W;Li S;Hung I;Gan Z;Xu J;Deng F
• 通讯作者: Deng F

Surprising Rigidity of Functionally Important Water Molecules Buried in the Lipid Headgroup Region.

• DOI: 10.1021/jacs.2c02145
• 发表时间: 2022-05-04
• 期刊: JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
• 影响因子: 15
• 作者: Zhang, Rongfu;Cross, Timothy A.;Peng, Xinhua;Fu, Riqiang
• 通讯作者: Fu, Riqiang

Implementing High Q-Factor HTS Resonators to Enhance Probe Sensitivity in 13C NMR Spectroscopy.

采用高 Q 因子 HTS 谐振器来增强 13C NMR 光谱中的探头灵敏度。

• DOI: 10.1088/1742-6596/2323/1/012030
• 发表时间: 2022
• 期刊: Journal of physics. Conference series
• 作者: Thomas,JN;Johnston,TL;Litvak,IM;Ramaswamy,V;Merritt,ME;Rocca,JR;Edison,AS;Brey,WW
• 通讯作者: Brey,WW