Animal Core

动物核心

• 批准号: 8148090
• 负责人: Gregory D Fink
• 金额: $ 15.5万
• 依托单位: MICHIGAN STATE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2003
• 资助国家: 美国
• 起止时间: 2003-07-10 至 2013-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8148090
• 关键词: Address; Animal Experimentation; Animal Housing; Animal Husbandry; Animals; Arteries; Blood Pressure; Cardiovascular Physiology; Cardiovascular system; Catheters; Chronic; Data; Ensure; Excision; Health; Human Resources; Hypertension; Implant; In Vitro; Individual; Laboratory Animals; Measurement; Methods; Michigan; Monitor; Operative Surgical Procedures; Physiological; Production; Protocols documentation; Rattus; Research Personnel; Resources; Schedule; Systolic Pressure; Tail; Techniques; Time; Tissue Sample; Tissues; Universities; Veins; animal resource; design; hemodynamics; in vivo; instrumentation; interest; programs; sample collection

This core unit serves as a central resource for normal and hypertensive animals used by all investigators in the Program Project. Core personnel are responsible for continuing and up-to-date assessment of animal requirements for all Projects. They ensure timely ordering, delivery, and proper housing of animals. All animal surgery (except some post-mortem tissue removal) is conducted and/or supervised by Core B personnel. This includes production of DOCA-salt and S6c-induced hypertension and appropriate control or sham-operated rats, most routine tissue removal for in vitro analysis, and instrumentation of rats for chronic hemodynamic monitoring. All animals used in the Program Project have their blood pressure carefully and reliably quantified by Core B personnel either directly (implanted arterial catheter or radiotelemetry transmitter) or indirectly (systolic pressure by tail-cuff sphygmomanometry) prior to or as part of each experimental protocol. Daily animal husbandry, health surveillance and record keeping also are the responsibility of Core B personnel, in association with the staff of University Laboratory Animals Resources (ULAR) of Michigan State University. Core B personnel also are responsible for developing and implementing new physiological techniques for evaluating cardiovascular function in rats. A major advantage of the Animal Core is that it facilitates the most efficient use of animals by Program Investigators. It is our usual practice to schedule tissue sample collection from an individual rat in such as way as to allow at least two, and often three or more, investigators to obtain needed tissues from the same animal. Our in vivo protocols also are designed to maximize the amount of data collected from a single animal. This is achieved through use of long-term repeated measurements of cardiovascular parameters, rather than studying separate groups of rats at each time point of interest. Often at the end of chronic in vivo protocols, tissue samples from the rats are distributed to one or more other Program investigators. Thus, through careful planning, and refinement of our methods, we are able to significantly reduce the number of animals used to address our scientific aims. However, it is not yet possible to replace animal experimentation as part of our efforts to understand the control of artery and vein function.

该核心单元是所有研究人员在 计划项目。核心人员负责继续对动物进行最新评估 所有项目的要求。它们确保及时订购，分娩和适当的动物住房。所有动物 手术（除了某些验尸后除外）由核心B人员进行和/或监督。这 包括生产DOCA盐和S6C诱导的高血压以及适当的控制或假手术 大鼠，大多数常规的组织去除用于体外分析的大鼠，以及用于慢性血流动力学的大鼠的仪器 监视。该计划项目中使用的所有动物都仔细且可靠地量化了血压 由核心B人员直接（植入动脉导管或放射性驱动器发射器）或间接 （通过尾部脱落的血压计计算压力）在每种实验方案的一部分之前或作为一部分。日常的 畜牧业，健康监视和记录保存也是核心B人员的责任 与密歇根州立大学的大学实验动物资源（ULAR）的工作人员有关。 核心B人员还负责开发和实施新的生理技术 评估大鼠心血管功能。动物核心的主要优势是它促进了最大的 计划调查人员有效使用动物。安排纸巾样品收集是我们通常的做法 从单个老鼠的方式中，以至于允许至少两个，通常三个以上的调查人员来获得 来自同一动物的需要组织。我们的体内协议也旨在最大化数据量 从一只动物中收集。这是通过使用长期重复测量的 心血管参数，而不是在每个感兴趣的时间点研究单独的大鼠组。经常在 慢性体内方案的结束，大鼠的组织样品分布到一个或多个其他程序 调查人员。因此，通过仔细的计划和对我们方法的完善，我们能够显着 减少用于解决我们科学目标的动物数量。但是，尚无法更换 动物实验是我们了解动脉和静脉功能控制的努力的一部分。