Acquisition of Shared Thermoneutral Rodent Housing Resources

获取共享的热中性啮齿动物住房资源

• 批准号: 10734172
• 负责人: Alan Daugherty
• 金额: $ 18.21万
• 依托单位: UNIVERSITY OF KENTUCKY
• 依托单位国家: 美国
• 财政年份: 2023
• 资助国家: 美国
• 起止时间: 2023-07-15 至 2024-07-14
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10734172
• 关键词: Address; Affect; Animal Experimentation; Animal Housing; Animals; Area; Awareness; Basal metabolic rate; Behavioral; Biomedical Research; Blood Pressure; Breeding; Calibration; Cardiac; Chronic; Complement; Core Facility; Data; Dedications; Disease; Disease Outcome; Eating; Energy Metabolism; Environmental Impact; Equipment; Faculty; Floor; Funding; Goals; Health; Heart Rate; House mice; Housing; Human; Immune response; Individual; Intervention; Kentucky; Laboratories; Laboratory Animals; Laboratory mice; Literature; Maintenance; Metabolic Diseases; Modeling; Modernization; Mus; Nerve Degeneration; Outcome Study; Outcomes Research; Pathologic; Pathology; Physiological; Plants; Procedures; Process; Productivity; Publications; Research; Research Activity; Research Design; Research Personnel; Research Support; Resistance; Resources; Rodent; Services; Skeletal Muscle; Sleep; Stress; System; Temperature; Translations; United States National Institutes of Health; Universities; Vascular Dementia; animal facility; animal resource; behavioral study; circadian; cold stress; cost; design; experimental study; fetal programming; genetic manipulation; human disease; immune function; muscle hypertrophy; operation; research facility; response; square foot; tumor growth; ventilation

PROJECT SUMARY/ABSTRACT The need to house and maintain laboratory rodents under conditions of thermoneutrality, i.e., at the ambient temperature at which energy expenditure is limited to that required to maintain basal metabolic rate, is increasingly recognized as critical to achieving rigorous research outcomes. Evidence of temperature effects on a vast array of physiological functions and pathologies spanning energy expenditure, immune function and response, blood pressure, heart rate, sleep, food intake, tumor growth and resistance, and more have raised awareness of the need to factor thermoneutrality into research designs to enhance the validity of research findings and their translation to understand and enhance human health. To accommodate recent and ongoing campus expansion of research capabilities for conducting biomedical studies in mice under thermoneutrality, the University of Kentucky (UK) proposes to establish a Thermoneutrality Suite in its newest vivarium in the recently constructed Healthy Kentucky Research Building (HKRB). The 3,000 sq. ft. HKRB vivarium supports a 265,000 sq. ft. six-floor state-of-the-art biomedical research facility that opened in 2018 and is contiguous to animal facilities in two adjacent modern research buildings. The long-term goal of this equipment application is to enhance and modernize animal research support operations managed by the UK Division of Laboratory Animal Resources (DLAR) by equipping a three-room Thermoneutrality Suite (970 sq. ft. of space) with dedicated advanced thermoneutrality caging. The proposed suite, to be operated by DLAR and overseen by the Associate Vice President for Research Core Facilities, will achieve the specific aim to permit both the breeding and housing of mouse colonies at thermoneutrality by acquiring six mobile Solace Zone IVC caging systems to be installed in a 385 sq. ft. space adjoining two rooms equipped for experimentation under thermoneutral study conditions. This request to the NIH will complement acquisition of separately acquired thermoneutral experimental equipment to be consolidated in two adjacent rooms, but not part of this funding request. The completed suite will elevate DLAR’s research-supporting operations for NIH-funded investigators who studies require access to three Power Scientific Inc. model RIS70SD rodent experimental chambers to support behavioral studies and four Actimetrics Clocklab Chambers suitable for performing circadian studies, one of which is already in place. The HKRB vivarium is a modern, high-end facility. This facilities upgrade will enable new research efficiencies and provide enhanced rigor to researchers whose study objectives require carefully calibrated environmental conditions through an integrated research support operation. Addressing this need for cutting-edge mobile animal housing units will impact diverse NIH-funded studies spanning cardiac arrythmias, vascular dementia and neurodegeneration, skeletal muscle hypertrophy, metabolic disease, fetal programming, and more to produce more rigorous study outcomes. These areas represent some of UK’s most prominent areas of research activity conducted by a highly productive and collaborative faculty.

项目sumary/摘要 需要在温度条件下容纳和维护实验室啮齿动物，即在环境中 能源支出仅限于维持基本代谢率所需的温度，为 越来越公认的对于实现严格的研究成果至关重要。温度影响的证据 在跨越能量消耗，免疫功能和的一系列生理功能和病理上 反应，血压，心率，睡眠，食物摄入量，肿瘤生长和抗药性以及更多增加 意识到需要将热过屈的需求纳入研究设计以提高研究的有效性 发现及其翻译以了解和增强人类健康。适应最近和正在进行的 校园扩展研究能力，用于在热反态下在小鼠中进行生物医学研究， 肯塔基大学（英国）的提议，在其最新的Vivarium中建立一个热术套件 最近建造了健康的肯塔基州研究大楼（HKRB）。 3,000平方英尺的HKRB Vivarium支持A 265,000平方英尺的六层最先进的生物医学研究设施，于2018年开业，与 两座相邻现代研究建筑中的动物设施。该设备应用的长期目标是 增强和现代化由英国实验室部门管理的动物研究支持操作 动物资源（DLAR）通过配备三居室热套房（空间970平方英尺） 专用的晚期热笼。拟议的套房，由DLAR和海外运营 研究核心设施副副总裁将实现具体目标，以允许 通过获取六个移动慰藉区IVC笼子，在热自治处的繁殖和住房 安装在385平方英尺的空间中的系统，与两个房间相邻，可在 热中性研究条件。对NIH的此请求将完成单独收购的收购 在两个相邻的房间中巩固的热负性实验设备，但没有该资金的一部分 要求。完整的套件将提升DLAR的研究支持NIH资助的研究人员的研究支持 谁的研究需要访问三个Power Scientific Inc. Model RIS70SD啮齿动物实验室 支持行为研究和四个适用于进行昼夜研究的Actimetrics Clocklab Chambers， 其中之一已经到位。 HKRB Vivarium是现代的高端设施。此设施升级将 实现新的研究效率，并为研究目标需要的研究人员提供了增强的严格性 通过集成的研究支持操作仔细校准环境条件。解决这个问题 尖端的移动动物住房单位的需求将影响潜水员NIH资助的心脏研究 芳香族，血管痴呆和神经退行性，骨骼肌肥大，代谢疾病，胎儿 编程，更多，以产生更严格的研究成果。这些领域代表了英国最多的地区 高产和协作的教师进行的研究活动的重要领域。