Vivarium Modernization with Digital Ventilated Cages to Enhance Research Capacity and Reproducibility, and Provide Cage Environment Monitoring for Improved Operational Efficiency and Animal Welfare

采用数字通风笼进行现代化改造，以提高研究能力和再现性，并提供笼环境监测，以提高运营效率和动物福利

• 批准号: 10533591
• 负责人: Theodore F Taraschi
• 金额: $ 40万
• 依托单位: THOMAS JEFFERSON UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2022
• 资助国家: 美国
• 起止时间: 2022-08-01 至 2023-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10533591
• 关键词: 2019-nCoV; 5 year old; Address; Administrator; Aging; Air; Air Movements; Animal Experimentation; Animal Housing; Animal Model; Animal Welfare; Animals; Biomedical Research; Censuses; Communicable Diseases; Communities; Computer software; Containment; Data; Discipline; Electronics; Engineering; Ensure; Environment; Equipment; Food; Funding; Future; General Practices; Goals; Health; Home; Housing; Human; Human Resources; Humidity; Individual; Infectious Agent; Infectious Diseases Research; Intervention; Investments; KAI1 gene; Laboratory Animal Production and Facilities; Lighting; Lymphocytic choriomeningitis virus; Modernization; Monitor; Noise; Personal Satisfaction; Powassan virus; Process; Publications; Rabies; Reporting; Reproducibility; Research; Research Personnel; Research Support; Risk Reduction; Rodent; Schedule; Science; Secure; Services; Severe Acute Respiratory Syndrome; Specimen Handling; Standardization; Strategic Planning; Stress; System; Temperature; Time; Tissue Sample; Universities; Update; Vaccinia; Variant; Water; Work; animal care; animal data; animal facility; base; biosecurity; coronavirus disease; detection platform; digital; experimental study; high efficiency particulate air filter; humane endpoint; improved; influenzavirus; meetings; operation; pathogen; programs; research study; response; sample collection; sensor; tissue processing; ventilation; vibration

ABSTRACT We are seeking support to purchase and install an integrated digital ventilated cage system to enhance operational efficacy and modernize and strengthen the research-supporting operations of an existing, more than twenty-five-year-old shared-use ABSL3 facility. This FOA is timely, since the addition of the integrated Digital Ventilated Cage (DVC®) system we are requesting would synergize with our ongoing institutional strategic plan to modernize existing vivarium facilities, and have broad benefits for the institutional research community. The static microisolator caging system currently in use is suboptimal and antiquated and cannot meet the current or future needs of the large number of investigators from different disciplines performing ABSL3 work with animals infected with SAR2-Covid, SARS-Covid variants and other BL3 agents. The DVC® is a unique and revolutionary home-cage monitoring system composed of a mix of electronics and software components to collect a set of information directly from the home cage. We selected this system with the goals of 1) improving the shared-use facility operational efficiency and 2) enhancing animal welfare management. Furthermore, 3) the unique detection system collects extrinsic environmental conditions (temperature, humidity, noise and vibration, human intervention, etc.) in the home cages. These factors are likely to have effects on experiments using animals and reporting them in publications as a general practice could contribute to improved research quality (rigor and reproducibility). The modernization of an outdated ABSL3 facility to a more secure one containing a state-of-the-art integrated digital ventilated cage system in two animal housing rooms. This equipment modernization will combine and potentially synergize with institutionally-funded modernization efforts slated to be completed within the next six months – for example, the modernized animal housing caging system will be located in rooms adjacent to and with direct access to a modernized BL3 suite for tissue processing and specimen processing. Therefore, our proposed equipment modernization is a critical step towards meeting the needs of current and future investigators from diverse disciplines using rodent animal models for research involving an increasing number of BL3 pathogens.

抽象的 我们正在寻求支持购买和安装集成的数字通风笼系统 提高运营效率，现代化并加强了研究支持的操作 现有的，超过25岁的共享使用ABSL3设施。这个foa是及时的，因为 我们要求的集成数字通风笼（DVC®）系统的添加将协同作用 有了我们正在进行的机构战略计划，以现代化现有的Vivarium设施，并拥有广泛的 对机构研究社区的好处。目前正在 使用次优和过时，无法满足大量的当前或将来的需求 来自不同学科的研究人员，与SAR2-Covid感染的动物一起工作， SARS-COVID变体和其他BL3代理。 DVC®是一个独特而革命性的家居式 监视系统由电子和软件组件组成，以收集一组 直接从家居笼子中的信息。我们选择了该系统的目标1） 共享使用设施的运营效率和2）增强动物福利管理。此外， 3）独特的检测系统收集外部环境条件 （温度，湿度，噪声和振动，人类干预等）在家庭笼中。这些 因素可能会对使用动物的实验产生影响，并将其报告为出版物 一般实践可能有助于提高研究质量（严格和可重复性）。 过时的ABSL3设施的现代化为一个更安全的设施，其中包含最先进的设施 在两个动物外壳中的集成数字通风笼系统。此设备现代化 将结合并可能与机构资助的现代化努力结合在一起 在接下来的六个月内完成 - 例如，现代化的动物外壳笼系统 将位于与纸巾相邻的房间中，直接访问现代化的BL3套件 处理和标本处理。因此，我们提出的设备现代化是关键 朝着满足潜水学科的当前和未来调查人员的需求的一步 用于研究的啮齿动物模型涉及越来越多的BL3病原体。