Development of an SPF Baboon Colony

SPF 狒狒群体的发展

• 批准号: 8117086
• 负责人: GARY L WHITE
• 金额: $ 69.99万
• 依托单位: UNIVERSITY OF OKLAHOMA HLTH SCIENCES CTR
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-07-15 至 2012-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8117086
• 关键词: Age; Animal Experimentation; Animal Model; Animals; Back; Behavioral; Biological Assay; Biomedical Research; Breeding; Communities; Cytomegalovirus; Data; Derivation procedure; Development; Funding; Goals; Growth and Development function; Hand; Herpesviridae; Human; Individual; Infant; Institution; Macaca; Maternal antibody; Measles; Modeling; Monkeypox; N.I.H. Research Support; National Heart, Lung, and Blood Institute; Papio; Primates; Protocols documentation; Recruitment Activity; Research; Research Personnel; Resources; Retroviridae; Simian virus 40; Specific Pathogen Frees; Testing; United States National Institutes of Health; Virus; aged; analog; baboon herpesvirus; behavioral health; experience; germ free condition; improved; male; nonhuman primate; programs

DESCRIPTION (provided by applicant): The baboon has long been used in biomedical research and, for certain applications, has considerable advantages over other primate species. As is well appreciated for macaques, viruses constituting the normal flora of research animals can have a considerable effect on research results and their validity. Baboons are known to harbor analogs of many of the herpesviruses and retroviruses known to infect humans and other primates. Despite many years of successful breeding of baboons by various institutions, the nascent SPF baboon program at OUHSC is the only one of its kind in the world. The overall goal of this program is to develop an active, self-supporting breeding colony of baboons that are free of all five known baboon herpesviruses, four retroviruses, SV40, measles and monkeypox and that are available primarily to support NIH- funded biomedical research programs throughout the US. To accomplish this goal we propose the following specific aims:1. Continue derivation and expansion of the SPF colony. Approximately 25 new infants will be recruited into the SPF program each year. During the requested funding period, initial recruits will reach breeding age and the SPF colony should begin to experience "internal additions" via infants born to SPF animals. 2. Improve the "behavioral health" of infants in the SPF program. Protocols for hand-rearing of infants will be progressively modified with professional guidance to improve the overall behavioral health of baboons in the SPF colony as they grow and mature. 3. Establish multi-aged breeding groups of SPF baboons in a new indoor/outdoor corral facility. When the new facility currently under construction is completed, SPF animals will be moved into it to form several breeding groups consisting of animals of varied ages. 4. Provide SPF baboons to NIH-funded researchers. We are now saving back a limited number of SPF infant males to support the critical needs of the NHLBI/NIH and a few other NIH supported research programs that have critical need for SPF baboons. As the colony begins to mature to breeding status, excess males will be made available to the research community for use in NIH-supported biomedical research programs. Animals culled from the program due to sero-conversion to one virus will also be made available for use in research on projects that can use baboons with the specific virus that triggered the culling of the individual baboon. 5. Establish PCR assays for select viruses to analyze breaks in SPF status due to these viruses. CMV and HVP1 have been responsible for the majority of infants culled from the SPF program. In order to identify infants infected with these viruses before maternal antibodies wane, we will develop PCR tests that can detect infected infants.

描述（由申请人提供）：狒狒长期以来一直在生物医学研究中使用，并且对于某些应用，比其他灵长类动物具有相当大的优势。正如猕猴所欣赏的那样，构成正常研究动物菌群的病毒可能会对研究结果及其有效性产生相当大的影响。众所周知，狒狒具有许多疱疹病毒和已知会感染人类和其他灵长类动物的逆转录病毒的类似物。尽管各种机构多年来成功地繁殖了狒狒，但OUHSC的新生SPF狒狒计划是世界上唯一同类的。该计划的总体目标是开发一个积极的自支撑繁殖殖民地的狒狒殖民地，这些殖民地均含有所有五种已知的狒狒疱疹病毒，四种逆转录病毒，SV40，麻疹和蒙基托克斯，并且主要用于支持NIH资助的生物医学研究计划整个美国。为了实现这一目标，我们提出以下特定目标：1。继续推导和扩展SPF菌落。每年将大约25名新婴儿招募到SPF计划。在要求的资金期间，初始新兵将达到繁殖年龄，SPF殖民地应开始通过SPF动物出生的婴儿体验“内部添加”。 2。改善SPF计划中婴儿的“行为健康”。通过专业指导，将逐步修改婴儿手工抚养的方案，以改善SPF殖民地狒狒在成长和成熟时的总体行为健康。 3。在新的室内/室外畜栏设施中建立多年级的SPF狒狒。当目前正在建设的新设施完成后，SPF动物将被移入其中，形成多个繁殖群，这些繁殖群包括各种年龄段的动物。 4。向NIH资助的研究人员提供SPF狒狒。现在，我们节省了有限数量的SPF婴儿雄性，以支持NHLBI/NIH的关键需求，以及其他一些对SPF狒狒非常需要的NIH支持的研究计划。随着殖民地开始成熟到繁殖状态，将向研究社区提供多余的男性，以用于NIH支持的生物医学研究计划。由于血清转化为一种病毒而被从该程序中剔除的动物也将用于研究，用于研究可以使用带有特定病毒的狒狒的项目，这些病毒触发了单个狒狒的剔除。 5。建立针对某些病毒的PCR分析，以分析由于这些病毒而分析SPF状态的断裂。 CMV和HVP1负责SPF计划中挑选的大多数婴儿。为了在母体抗体减弱之前鉴定感染这些病毒的婴儿，我们将开发可检测受感染婴儿的PCR检测。