DEVELOPMENT OF AN SPF BABOON COLONY

SPF 狒狒群体的发展

• 批准号: 6421723
• 负责人: GARY L WHITE
• 金额: $ 35.34万
• 依托单位: UNIVERSITY OF OKLAHOMA HLTH SCIENCES CTR
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-07-15 至 2007-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6421723
• 关键词: Herpesviridae; Retroviridae; animal breeding; animal colony; baboons; enzyme linked immunosorbent assay; germ free condition; infant animal; juvenile animal; leukocytes; polymerase chain reaction; provirus; serology /serodiagnosis; simian virus 40; virus infection mechanism

DESCRIPTION (Provided by applicant): The baboon has long been used in biomedical research and for certain applications has considerable advantages over other primate species. Despite their widespread use in research, there is currently an acute shortage of baboons available for use. The UOHSC has the third largest baboon breeding colony in the U.S. and is committed to improving and expanding this national resource. A new corral breeding facility has just been completed to allow expansion of the existing colony to approximately twice the current size. As is well appreciated for macaques, viruses constituting the normal flora of research animals can have a considerable effect on research results and their validity. Baboons are known to harbor analogs of many of the herpesviruses and retroviruses known to infect humans and other primates. Despite many years of successful breeding of baboons by various institutions, no colonies of SPF baboons exist that are free of even one specific virus. The program proposed here will result in the beginning of a self-sustaining colony of baboons free of all known herpesviruses, four retroviruses, and SV40. To accomplish this goal, the investigators will establish serological and PCR tests for each of the 11 target viruses. These baboon viruses will include six herpesviruses [analogs of human herpes simplex virus (HSV), varicellazooter virus (VZV), cytomegalovirus (CMV), human herpesviruses 6 (HHV6), Epstein-Barr virus (EBV), and human herpesvirus 8 (HHV-8)), four retroviruses (simian foamy virus (SFV), simian retrovirus type D (SRV/D), simian immunodeficiency virus (SIV), and simian T cell lymphotropic virus (STLV)] and simian virus 40 (SV40). The investigators will recruit no fewer than 12 infant baboons into the SPF program in each of the first two years, and 14/year in years 3-5 of the program for a final total of at least 66 SPF baboons. Utilizing timed pregnancies, over a 1-2 week period, two infants will be removed from their dams within 12 hrs of delivery and hand-reared to six months of age. All infants will be repeatedly tested for each of the target viruses. At one year of age, larger social groups of four to six SPF animals will be formed. Beginning at two to three years of age, SPF animals will be integrated into larger socially compatible groups. These groups will eventually mature into small breeding harems of SPF animals. This approach will: 1) provide infants with an age-matched companion for socialization during their early period of development; 2) minimize opportunities for transmission of endogenous viruses to the infants from adult animals; and 3) allow the simultaneous elimination of many different viruses from SPF animals.

描述（由申请人提供）：狒狒长期以来被用于 生物医学研究和某些应用具有相当大的优势 超过其他灵长类动物。尽管它们在研究中广泛使用，但 目前可供利用的狒狒严重短缺。 UOHSC 有 美国第三大狒狒繁殖地，致力于 改善和扩大这一国家资源。新的畜栏养殖 设施刚刚竣工，可以将现有的殖民地扩展到 大约是当前尺寸的两倍。 正如猕猴所熟知的那样，构成猕猴正常菌群的病毒 研究动物可以对研究结果及其结果产生相当大的影响 有效性。众所周知，狒狒含有许多疱疹病毒的类似物 以及已知感染人类和其他灵长类动物的逆转录病毒。尽管许多 多年来各种机构成功繁殖狒狒，没有发现任何群体 存在的 SPF 狒狒甚至没有一种特定病毒。该计划 这里提出的建议将导致一个自给自足的殖民地的开始 狒狒不含所有已知的疱疹病毒、四种逆转录病毒和 SV40。 为了实现这一目标，研究人员将建立血清学和 PCR 对 11 种目标病毒中的每一种进行测试。这些狒狒病毒将包括 六种疱疹病毒 [人类单纯疱疹病毒 (HSV)、水痘病毒的类似物 病毒 (VZV)、巨细胞病毒 (CMV)、人类疱疹病毒 6 (HHV6)、 Epstein-Barr 病毒 (EBV) 和人类疱疹病毒 8 (HHV-8))，四种逆转录病毒 （猿猴泡沫病毒（SFV）、猿猴逆转录病毒 D 型（SRV/D）、猿猴 免疫缺陷病毒 (SIV) 和猿 T 细胞嗜淋巴细胞病毒 (STLV)] 和 猿猴病毒 40 (SV40)。调查人员将招募不少于12名 头两年每年将婴儿狒狒纳入 SPF 计划，并且 项目第 3-5 年每年 14 次，最终 SPF 总数至少为 66 狒狒。利用定时怀孕，在 1-2 周内生下两个婴儿 将在分娩后 12 小时内从水坝中移出并人工饲养 六个月大。所有婴儿都将接受针对每一项的重复测试 目标病毒。一岁时，有四到六个 SPF 的较大社交群体 动物将被形成。 SPF 动物从两到三岁开始 将融入更大的社会兼容群体。这些团体将 最终成熟为 SPF 动物的小型繁殖群。 这种方法将：1）为婴儿提供一个年龄匹配的陪伴者 发展早期的社会化； 2）最小化 内源性病毒从成人传播给婴儿的机会 动物; 3）允许同时消除多种不同的病毒 来自 SPF 动物。