Physiology Core

生理学核心

• 批准号: 8109204
• 负责人: David Francis Dolan
• 金额: $ 10.71万
• 依托单位: UNIVERSITY OF MICHIGAN AT ANN ARBOR
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-08-01 至 2012-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8109204
• 关键词: Action Potentials; Adopted; Affect; Animal Model; Animals; Arts; Auditory; Auditory Brainstem Responses; Auditory Physiology; Auditory system; Biochemical; Biological; Cavia; Cell physiology; Cells; Collaborations; Comparative Study; Data; Development; Education; Electrophysiology (science); Evaluation; Experimental Designs; Experimental Models; Faculty; Fostering; Future; Genetic; Genetic Models; Goals; Grant; Hand; Hearing; Homeostasis; Individual; Intervention; Investigation; Knock-out; Laboratories; Link; Measurement; Measures; Methodology; Methods; Michigan; Modeling; Molecular; Molecular Biology; Molecular Genetics; Mouse Strains; Mus; Mutation; Nerve; Noise; Physiological; Physiology; Population; Preparation; Procedures; Productivity; Publications; Quality Control; Reflex action; Research; Research Personnel; Research Project Grants; Resources; Schedule; Services; Structure; Techniques; Training; Transgenic Organisms; Universities; Work; base; data exchange; hearing impairment; improved; meetings; mouse model; novel; operation; otoacoustic emission; patch clamp; restoration; round window; statistics; success

The assessment of auditory function is essential for any study that analyzes or manipulates the auditory system in relation to genetics, homeostasis, protection, or restoration of hearing. The Auditory Physiology Core will provide this critical support. While the auditory brainstem response will be the primary measure of auditory sensitivity, physiology facilities and expertise will be available for more in-depth measures of auditory function, e.g..otoacoustic emissions, efferent reflex, round window noise, cochlear microphonics, cochlear whole-nerve action potentials, and endolymphatic potential. Two new services include "hands on" training in state-of-the-art methods and procedures for multi-channel cellular recordings and patch-clamp electrophysiology. The Auditory Physiology Core will also coordinate noise exposures of animals with respect to each investigator's needs for either temporary or permanent threshold shifts. The availability of physiological measurements will aid individual projects but also promote collaboration among investigators with widely varying expertise but with similar questions pertaining to auditory physiology. Together with the Cell /Molecular Core, this core will provide the essential link of genetic, molecular and biochemical data to auditory structure and function. The core will also work closely with the new Delivery Core D. The studies fostered by this new core will require physiological assessment to evaluate the success of the interventions. The results obtained from the procedures described in this core will be analyzed for statistical significance with the assistance of the Statistics Department at the University of Michigan. To promote efficient and successful Core operation, regularly scheduled meetings will discuss progress in research, emerging techniques and future directions. Furthermore, the Core will continue to offer resources to replace current animal models (such as the guinea pig) with mice which offer marked advantages by virtue of increasing availability of molecular and genetic information. Procedures are in place for scheduling and prioritization of services and quality control, and to coordinate the activities of the Cell/Molecular and Auditory Physiology Cores to facilitate an increased understanding of the molecular and genetic basis for hearing function.

听觉功能的评估对于任何分析或操纵听觉的研究都是至关重要的 与遗传学、体内平衡、保护或听力恢复有关的系统。听觉生理学 Core 将提供这一关键支持。虽然听觉脑干反应将是主要衡量标准 听觉敏感性、生理学设施和专业知识将可用于更深入的测量 听觉功能，例如耳声发射、传出反射、圆窗噪声、耳蜗微音学、 耳蜗全神经动作电位和内淋巴电位。两项新服务包括“动手” 多通道细胞记录和膜片钳的最先进方法和程序的培训 电生理学。听觉生理学核心还将协调动物的噪音暴露 尊重每个研究者对临时或永久阈值变化的需求。 生理测量的可用性将有助于单个项目，同时也促进合作 具有广泛不同专业知识但在听觉方面有类似问题的研究人员 生理。与细胞/分子核心一起，该核心将提供遗传、 听觉结构和功能的分子和生化数据。核心还将与 新的交付核心 D。该新核心所促进的研究将需要生理评估来评估 干预措施的成功。 从本核心中描述的程序获得的结果将进行统计分析 在密歇根大学统计系的协助下，这一结果具有重要意义。推广 高效、成功的核心运作，定期召开会议讨论研究进展， 新兴技术和未来方向。此外，核心将继续提供资源来替代 目前使用小鼠的动物模型（例如豚鼠）具有显着的优势 增加分子和遗传信息的可用性。已制定安排和安排程序 确定服务和质量控制的优先顺序，并协调细胞/分子和 听觉生理学核心有助于加深对听觉生理学分子和遗传基础的理解 听觉功能。