In Vivo Cellular and Molecular Imaging Centers (ICMICs)

体内细胞和分子成像中心 (ICMIC)

• 批准号: 8062803
• 负责人: ROBERT F MATTREY
• 金额: $ 6.17万
• 依托单位: UNIVERSITY OF CALIFORNIA, SAN DIEGO
• 依托单位国家: 美国
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-09-23 至 2013-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8062803
• 关键词: in vivo Cellular and Molecular Imaging Centers

The Unviersity of California, San Diego is committed to developing in-vivo imaging at the molecular and cellular level to answer basic molecular biology questions, to determine the molecular basis of drug action, interaction and resistance, to assess the effect of therapeutic interventions and to probe unique animal models at the molecular level of disease in general, and cancer in particular. The ultimate goal is to develop new diagnostic and therapeutic tools to detect and distinguish aggressive from indolent lesions in order to treat patients at the early stages of cancer development. Also critical is to assess therapeutic effects when therapy is directed at molecular targets such as enzyme inhibition or interference with specific pathways. The centralized space in the new Moores UCSD Cancer Center (MCC) that also houses our rodent imaging facility adjacent to the vivarium will group the ICMIC team with other cancer investigators. We have built the ICMIC along side the NCI funded NanoTumor Center within the MCC to maximize efficiency and synergism. The P20 award 5 years ago began our multidisciplinary collaboration. Over the last five years we have submitted more than 10 new ideas as R01-type projects in the P50s and many development projects in the P20 and P50 submissions. Eight of those ideas (7 for projects and 1 developmental project) have now been funded by the NIH, DOD, or DOE. We present here 5 new projects and one that was submitted previously that has been revised. The projects submitted in this P50 application focus on the interface between imaging and therapy. Project 1 aims to detect matrix metalloproteinase in tumors, a sign of tumor aggressiveness, with ultrasound contrast agents. They plan to take advantage of the fact that fluorocarbon emulsions are visible only when combined to induce aggregation at the time the emulsion is exposed to the enzyme of interest. Project 2 aims to label a platinum carrying polymer for PET and SPECT imaging of a cancer chemotherapeutic currently in clinical trial. Their goal is to provide the clinicians with drug kinetics and propose to increase the agent's therapeutic index by targeting it as the vitamin B-12 receptor. Project 3 proposes a clever nanoparticle self-assembly system to target angiogenesis, also a sign of tumor aggressiveness, for imaging and therapy. The Developmental Projects represent cutting edge research that focus on cell labeling, gene delivery, and optical imaging of stem cell cancers. We also focus this ICMIC on Career Development and present three applicants for training in molecular imaging. The P.l.s of one major project and all 3 Developmental Projects are junior faculty from Pathology (1), Medicine (3) and the Cancer Center (1) that are mentored by seasoned investigators. The Institutional support is significant. We have been provided prime space in the cancer center, all rodent imaging equipment are located in the cancer center adjacent to the vivarium, and key personnel.

圣地亚哥的加利福尼亚州的揭晓致力于在分子和细胞水平开发体内成像，以回答基本的分子生物学问题，以确定药物作用，相互作用和抗性的分子基础，以评估治疗干预措施的效果，并在一般疾病和癌症的分子疾病中探测独特的动物模型。最终目标是开发新的诊断和治疗工具，以检测和区分侵略性与懒惰病变，以便在癌症发展的早期治疗患者。当治疗针对分子靶标，例如酶抑制或干扰特定途径时，也要评估治疗作用。 新Moores UCSD癌症中心（MCC）的集中空间还设有我们的啮齿动物成像设施，该设施与Vivarium相邻，将与其他癌症研究者分组。我们已经在NCI资助的MCC内建立了ICMIC，以最大程度地提高效率和协同作用。 5年前，P20奖开始了我们的多学科合作。在过去的五年中，我们提交了10多个新想法，因为P50中的R01型项目以及P20和P50提交的许多开发项目。这些想法中有八个（项目和1个发展项目）现已 由NIH，DOD或DOE资助。我们在这里提出了5个新项目，这是一个已修订的项目。该P50应用程序中提交的项目重点介绍了成像和治疗之间的接口。项目1旨在检测肿瘤中基质金属蛋白酶，这是肿瘤侵袭性的迹象， 具有超声对比剂。他们计划利用这样一个事实，即仅在合并时才能看到氟化合物乳剂在乳液暴露于感兴趣酶时诱导聚集。项目2旨在将载有聚合物的铂载体标记用于临床试验中的癌症化学治疗剂的SPECT成像。他们的目标是为临床医生提供药物动力学，并建议通过将其作为维生素B-12受体来增加药物的治疗指数。项目3提出了一个聪明的纳米颗粒自组装系统来靶向血管生成，也是肿瘤侵袭性的标志，用于影像学和治疗。发展项目代表了尖端研究，该研究的重点是细胞标记，基因递送和干细胞癌的光学成像。 我们还将这种ICMIC重点放在职业发展上，并为分子成像培训的三名申请人提供。一个主要项目和所有3个发展项目的P.L.是病理学（1），医学（3）和癌症中心（1）的初级教师，这些教师均由经验丰富的研究人员指导。 机构支持很大。我们已经在癌症中心提供了主要空间，所有啮齿动物成像设备都位于毗邻Vivarium的癌症中心和关键人员。