Consolidated Biomedical Core Facilities Supporting a Center on Emerging and Zoonotic Infectious Disease Research

支持新发和人畜共患传染病研究中心的综合生物医学核心设施

• 批准号: 10372482
• 负责人: Bonnie Rush
• 金额: $ 343.1万
• 依托单位: KANSAS STATE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2021
• 资助国家: 美国
• 起止时间: 2021-09-20 至 2026-05-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10372482
• 关键词: Animal Diseases; Animal Model; Biomedical Research; CRISPR/Cas technology; Cell Separation; Cells; Centers of Research Excellence; Communicable Diseases; Complex; Computers and Advanced Instrumentation; Confocal Microscopy; Core Facility; DNA; Development; Discipline; Elements; Environment; Faculty; Floor; Flow Cytometry; Fostering; Image; Individual; Infectious Diseases Research; Infrastructure; Institution; Kansas; Laboratories; Location; Modernization; Molecular Analysis; Molecular and Cellular Biology; Nucleic Acids; Pathology; Phase; Positioning Attribute; Primary Health Care; Productivity; Research; Research Infrastructure; Research Institute; Research Personnel; Research Project Grants; Research Training; Science; Services; Technology; Time; Tissues; Training; Training Support; Universities; Zoonoses; arthropod-borne; biodefense; biosafety level 4 facility; biosecurity; clinical care; experimental study; flexibility; in vivo imaging; laser capture microdissection; member; microscopic imaging; next generation sequencing; research facility; transcriptome sequencing

SUMMARY: Kansas State University (KSU) is well-positioned to become the preeminent institution to advance the discovery and development of biosecurity strategies for emerging and zoonotic infectious diseases. With KSU’s Biosecurity Research Institute (BSL-3), USDA’s Arthropod-Borne Animal Disease Research Unit, and USDA’s National Bio and Agro-Biodefense Facility (BSL-3/BSL-4), KSU is the only U.S. university with a full continuum of biosecurity level 1 (BSL-1) through BSL-4 facilities collocated on one campus. The proposed core research facility represents a critical component of our research infrastructure to support infectious disease studies and provide direct support of both KSU’s 2020 COBRE center and collocated federal facilities. The core facility will strategically combine five key disciplines: animal model/pathology, molecular and cellular biology, microscopic imaging, flow cytometry and cell sorting, and next-generation sequencing. Investigators at KSU and in the region rely on these technologies to perform high-impact research and complete experiments outlined in current and future research projects. However, existing facilities for these key technologies are inadequate in size, location, function, and flexibility. Existing laboratories are isolated from each other, spread across three buildings, and in some cases, are hosted by individual faculty members, which limits access and research productivity. The proposed core-facility suite is the final element of a three-phase renovation of the KSU veterinary complex. Phase 1 delivered a contemporary 220-seat auditorium (8,200 GSF) adjacent to Mosier Hall. Phase 2 replaced an outdated auditorium with a primary-care clinical training facility occupying the 1st floor of Mosier Hall. Phase 3, the focus of this application, proposes to build a collaborative, university-wide core research facility occupying the 2nd floor of the deconstructed auditorium space. Currently, the 2nd floor is 5,000 GSF of laboratory-conditioned shell space. This proposal will strengthen our research capacity and infrastructure by creating a centrally organized, integrated technology pipeline, with proximate expert assistance and training support to facilitate efficient use of contemporary technology in confocal microscopy, live-cell and in vivo imaging, laser capture microdissection, flow cytometry, cell sorting, DNA/RNA sequencing and CRISPR technology. The proposed purpose-built, core-facility suite will assemble state-of-the-art technologies in a single location to deliver efficient, coordinated services for academic, corporate and federal researchers in imaging and molecular analyses, thereby providing a complete range of services from whole tissues to single-cell nucleic acid analyses. A dedicated modern biomedical research facility with advanced instrumentation and technical support will foster collaborative, synergistic and transdisciplinary science. This is critical to promoting a robust research and training environment where researchers can answer the most challenging and urgent biomedical questions of our time.

摘要：堪萨斯州立大学（KSU）有充分的位置成为促进的杰出机构 针对新兴和人畜共患疾病的生物安全策略的发现和发展。和 KSU的生物安全研究所（BSL-3），USDA的节肢动物传播动物疾病研究部门和 USDA的国家生物和农业生物化设施（BSL-3/BSL-4），KSU是唯一一所拥有完整的美国大学 通过BSL-4的设施在一个校园中置于BSL-4设施的生物安全性1（BSL-1）的连续体。提出的核心 研究机构是我们研究基础设施的关键组成部分，以支持传染病 研究并直接支持KSU的2020年毛to中心和联邦设施并置。核心 设施将战略性地结合五个关键学科：动物模型/病理学，分子和细胞生物学， 微观成像，流式细胞术和细胞分选以及下一代测序。 KSU和 在该地区，依靠这些技术来进行高影响研究和完整的实验 当前和未来的研究项目。但是，这些关键技术的现有设施不足 尺寸，位置，功能和灵活性。现有的实验室彼此隔离，分布在三个 建筑物以及某些情况下由个人教职员工主持，这限制了访问和研究 生产率。拟议的核心式套件是KSU三相改革的最终要素 兽医络合物。第1阶段提供了与Mosier Hall相邻的现代220个座位的礼堂（8,200 GSF）。 第2阶段用一楼的一楼取代了过时的礼堂 Mosier Hall。第三阶段，该应用程序的重点是建立合作，全大学核心的建议 研究机构占据了解构礼堂空间的2楼。目前，2楼是5,000 实验室条件的壳体空间的GSF。该建议将增强我们的研究能力和基础设施 通过创建一个由中央组织，集成的技术管道，并在附近的专家协助和培训中 支持在共聚焦显微镜，活细胞和体内成像中有效利用当代技术的有效利用， 激光捕获显微解剖，流式细胞仪，细胞分选，DNA/RNA测序和CRISPR技术。这 拟议的专用，核心式套件将在一个地点组装最新技术 为学术，企业和联邦研究人员提供成像和分子研究人员的高效，协调的服务 分析，从而提供从整个组织到单细胞核酸分析的完整服务。 具有高级仪器和技术支持的专用现代生物医学研究机构将促进 协作，协同和跨学科科学。这对于促进强大的研究和培训至关重要 研究人员可以回答我们这个时代最挑战和紧急的生物医学问题的环境。