In Vivo Cellular and Molecular Imaging Centers (ICMICs)

体内细胞和分子成像中心 (ICMIC)

• 批准号: 7892611
• 负责人: ROBERT F MATTREY
• 金额: $ 1.74万
• 依托单位: UNIVERSITY OF CALIFORNIA, SAN DIEGO
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-06-01 至 2011-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7892611
• 关键词: Vivo; Cellular; Molecular; Imaging; Centers

This proposal focuses on differences in the susceptibility to genotoxicants of early and late stage embryos with respect to embryological development. Grass shrimp will be used as a model organism to characterize the difference in susceptibility of early and late stage embryos to contaminant induced DNA damage. Grass shrimp embryos have been used for toxicity testing. Adult grass shrimp will be exposed to sediments and food containing chromium, mercury, or polycyclic aromatic hydrocarbons (PAH?s). Embryo stages produced from control and genotoxicant exposed adults will be followed through the various stages of development. DNA strand breakage and repair will be measured using the comet assay, including modifications for quantification of specific lesions and crosslinks. A DNA repair enzyme will be amplified, cloned and sequenced. Based on this sequence, primers will be prepared and tested to begin studies on the expressi on of a DNA repair gene in early and late stage embryos. The significance of this work is that it provides a model for the study of genotoxicity. By working with grass shrimp embryos, which have well studied developmental processes, a quick reproductive cycle which is easily manipulated in the laboratory, and are routinely exposed to genotoxicants in contaminated environments, we can understand the processes which make early embryonic stages more sensitive to contaminants.

该提案的重点是在胚胎学发育中对早期和晚期胚胎的遗传毒性的敏感性差异。草虾将用作模型 有机体表征早期和晚期胚胎对污染物诱导的DNA损伤的敏感性差异。草虾胚胎已用于毒性测试。成年草虾将暴露于含有铬，汞或多环芳族烃（PAH？s）的沉积物和食物。由对照和遗传毒性暴露的成年人产生的胚胎阶段将在发育的各个阶段遵循。 DNA链断裂和修复将使用彗星测定法进行测量，包括修改特定病变和交联的修改。 DNA修复酶将被放大，克隆和测序。基于此序列，将准备并测试引物，以开始对早期和晚期胚胎中DNA修复基因的表达研究。这项工作的意义在于它提供了 研究遗传毒性的模型。通过使用对开发过程进行良好研究的草虾胚胎的工作，这是一个快速的生殖周期，在实验室中很容易操纵，并且通常会在受污染的环境中暴露于遗传毒性中，我们可以理解使早期胚胎阶段对污染物更敏感的过程。