Cell and Tissue Microscopy Core

细胞和组织显微镜核心

基本信息

批准号：
10454194
负责人：
JYOTI K JAISWAL
金额：
$ 13.49万
依托单位：
CHILDREN'S RESEARCH INSTITUTE
依托单位国家：
美国
项目类别：
财政年份：
2021
资助国家：
美国
起止时间：
2021-07-21 至 2026-05-31
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/10454194
关键词：
3-Dimensional Area Bioinformatics Biomedical Engineering Brain Brain Mapping Cell Communication Cells Cellular Structures Child Childhood Clinical Collaborations Computer software Conceptions Confocal Microscopy Data Data Analyses Defect Development Disease District of Columbia Doctor of Philosophy Educational workshop Electron Microscopy Electrons Embryo Ensure Equipment Farm Functional Imaging Funding Genetic Genetic Diseases Genomics Goals Hospitals Image Imaging Device In Situ Individual Injury Intellectual and Developmental Disabilities Research Centers Intellectual functioning disability Intervention Ions Laboratories Light Logistics Metabolic Microscope Microscopic Microscopy Mission Molecular Abnormality Monitor Neonatal Brain Injury Neurodevelopmental Disorder Noise Optics Organ Organism Phenotype Process Quality Control Research Research Personnel Research Support Resolution Scanning Scanning Electron Microscopy Services Signal Transduction Structural defect Structure Techniques Technology Tissues Training Training Programs United States National Institutes of Health Universities Validation Visualization Washington Work analytical tool animal imaging cost effective design digital pathology experience fetal human imaging image visualization imaging approach imaging platform in vivo in vivo imaging industry partner innovation instrument instrumentation investigator training light microscopy loss of function microscopic imaging multiphoton microscopy nanoscale neural circuit neural network neurobehavioral neuroregulation new technology next generation optogenetics quantitative imaging recruit research study symposium technology development temporal measurement three-dimensional visualization tool

项目摘要

ABSTRACT The Cell and Tissue Microscopy Core (CTMC) will support the DC-IDDRC researchers in their work that relies on the use of high-resolution optical and electron microscopic approaches to study cells and tissues. CTMC will offer access to state-of-the-art microscopy imaging and analytical tools to support research focused on pediatric diseases with an emphasis on neurodevelopmental disorders, fetal and neonatal brain injury and genetic diseases. CTMC will extend its services to researchers at Children’s National Hospital, George Washington University, Georgetown University and Howard University. Among these users, there is a growing need for tools that enable imaging at greater spatial and temporal resolution while also allowing functional imaging and deep as well as large area imaging. To support these needs CTMC continues to expand its services including widefield, confocal, live and multiphoton microscopy with optogenetic manipulation, light sheet microscopy, super resolution, correlative light and EM as well as large field 3D-EM. With these developments the core will continue to meet and also expand its mission to enable structural and functional imaging from nanoscale to organismal scale that are aimed at understanding and treating defects leading to IDDs. The core will also help develop and implement quantitative analytical approaches for image visualization and data analysis. The core will also participate in collaborative IDD-relevant projects that heavily rely on the use of newer technologies. A significant mission of the core will be to train next generation of researchers in the use of advanced microscopy approaches through offering hands on training, educational workshops organized by the core in collaboration with other companies and researches in the greater DC region. CTMC will also collaborate with other DC-IDDRC cores including the Human and Animal Imaging Core, Neurobehavioral Core and Genomics and Bioinformatics Core to serve projects with cross disciplinary needs. Through above endeavors CTMC aims to continue leveraging technological developments in microscopy to enable conception, design, and implementation of these developments to tackle challenges in the field of IDD research and further the mission of DC-IDDRC.

抽象的细胞和组织显微镜核心（CTMC）将支持DC-IDDRC研究人员的工作关于研究细胞和组织的高分辨率光学和电子显微镜方法。 CTMC 将提供获得最先进的显微镜成像和分析工具，以支持关注的研究小儿疾病，重点是神经发育障碍，胎儿和新生儿脑损伤以及遗传疾病。 CTMC将向乔治儿童国家医院的研究人员扩展其服务华盛顿大学，乔治敦大学和霍华德大学。在这些用户中，有一个增长需要以更大的空间和临时分辨率启用成像的工具，同时允许功能成像，深处以及大面积成像。为了满足这些需求，CTMC继续扩展包括宽阔的菲尔德，共聚焦，实时和多光子显微镜以及带有光学遗传操作的服务板显微镜，超级分辨率，相关光和EM以及大型磁场3D-EM。与这些发展核心将继续满足并扩大其使命以实现结构和功能从纳米级到有机尺度的成像，旨在理解和治疗缺陷 IDD。核心还将有助于开发和实施定量分析方法以进行图像可视化和数据分析。核心还将参加与IDD相关的合作项目，这些项目在很大程度上依赖使用新技术。核心的重要任务是培训下一代研究人员通过提供培训，教育研讨会的动手使用高级显微镜方法由核心与大型DC地区的其他公司和研究合作组织。 CTMC 还将与其他DC-IDDRC核心合作，包括人类和动物成像核心，神经行为核心和基因组学和生物信息学核心，可服务于具有跨学科需求的项目。通过上述努力，CTMC旨在继续利用显微镜中的技术发展启用这些发展的概念，设计和实施，以应对IDD领域的挑战研究并进一步执行DC-IDDRC的任务。