NANO-CHIP HIV DETECTION IN VULNERABLE POPULATIONS

弱势群体中的纳米芯片 HIV 检测

• 批准号: 8143481
• 负责人: Glen N. Gaulton
• 金额: $ 18.57万
• 依托单位: UNIVERSITY OF PENNSYLVANIA
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-09-15 至 2013-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8143481
• 关键词: AIDS/HIV problem; Abnormal coordination; Acoustics; Address; Biological Assay; Biological Markers; C-Peptide; Caring; Clinic; Clinical; Complex; Computer software; Coupling; Data; Detection; Development; Devices; Diagnosis; Diagnostic; Dimensions; Disease; Elderly; Electrodes; Enzyme-Linked Immunosorbent Assay; Evaluation; Face; Future Generations; Goals; HIV; HIV Envelope Protein gp120; HIV Infections; Hand; Health; Health Services Accessibility; Health Status; Healthcare; Home environment; Individual; Insulin; Interleukin-6; Intervention; Laboratories; Liquid substance; Measurement; Measures; Medical; Metabolic; Methods; Modeling; Monitor; Outcome; Patients; Pharmaceutical Preparations; Phase; Population; Procedures; Quality of life; Reaction; Reaction Time; Sampling; Sensitivity and Specificity; Serum; System; Technology; Time; Validation; Viral load measurement; Visit; Vulnerable Populations; base; biochip; computerized data processing; cost; density; design; home based service; immune function; improved; monitoring device; nano; nanosensors; new technology; novel; novel strategies; older patient; point of care; response; sensor

DESCRIPTION (provided by applicant): We propose to develop a new intervention model directed toward the coordination and simplification of HIV treatment which has particular relevance to older persons and other, less mobile, populations. This model relies on the development of a novel Bio-nanosensor (BNS) diagnostic/treatment monitoring technology that will provide both health workers as well as patients with important medical information regarding disease status on a real-time basis in the home setting. This new technology will enable the creation of a new HIV/AIDS management system that will result in more efficient and effective monitoring and treatment of HIV infection and its complications. These goals are achieved through the following specific aims: Specific Aim 1: Develop a Novel Piezoelectric Bio-nanosensor Device to Detect HIV In this specific aim, we propose to develop a piezoelectric Bio-nanosensor (BNS) device for the rapid (10-15 min) fluid-phase detection of HIV based on the presence of the biomarkers gp120 and p24, and of intact HIV virus. We will then optimize assay sensitivity, specificity and dynamic range, and validation this device against the current laboratory-based ELISA/SPR detection format. Milestone: The development of a piezoelectric BNS assay with the ability to detect gp120 and p24 in <15 min reaction time with a limit of detection <100 pg/ml, while using no more than 10 -100 <l of fluid sample. Specific Aim 2: Design and Develop a BNS assay for Multiple Biomarkers Associated with HIV/AIDS Once the design of the basic BNS device format and HIV detection methods are established in Specific Aim 1, we will develop individual biochips optimized for sensing additional biomarkers that are routinely assessed in HIV infected patients: including CD4, insulin, C-peptide, IL-6 and HbA1C, and apply these to blood and serum analysis. This BNS assay will thus provide a specific health profile of HIV/AIDS patients. BNS optimization will include determining individual reaction conditions for each biomarker, and relevant operating conditions such as: size of electrode, packing density, optimum dimensions, acoustic mode of interaction, software and hardware development for monitoring multiple sensors and signal processing. Milestone: Development of a BNS system for simultaneous detection of the biomarkers gp120, p24, CD4, insulin, C-peptide, IL-6 and HbA1C at picogram sensitivity with an anticipated assay cost of $5 - $10/sample PUBLIC HEALTH RELEVANCE: Current care strategies for older patients (the number of older persons with HIV infection is forecast to augment in the years to come) with HIV infection as well as other conditions face challenges associated with highly complex treatment paradigms that lack coordination and thus often render care less effective. With multiple drugs and multiple problems, such patients are forced to endure a lower quality of life marked by the high possibility of treatment complications and diminished medical outcomes. In order to address this need, we propose an intervention model (an original bio-nanosensor detection technology) directed toward the coordination and simplification of HIV treatment, particularly in older persons suffering from multiple conditions.

描述（由申请人提供）：我们建议开发一种新的干预模型，旨在协调和简化艾滋病毒治疗，该模型与老年人和其他流动性较差的人群特别相关。该模型依赖于新型生物纳米传感器（BNS）诊断/治疗监测技术的开发，该技术将为卫生工作者和患者提供有关家庭环境中疾病状态的实时重要医疗信息。这项新技术将有助于创建新的艾滋病毒/艾滋病管理系统，从而更有效地监测和治疗艾滋病毒感染及其并发症。这些目标是通过以下具体目标实现的： 具体目标 1：开发新型压电生物纳米传感器设备来检测 HIV 在这一具体目标中，我们建议开发一种压电生物纳米传感器 (BNS) 设备，用于快速 (10-15 min) 基于生物标志物 gp120 和 p24 以及完整 HIV 病毒的存在进行 HIV 液相检测。然后，我们将优化检测灵敏度、特异性和动态范围，并根据当前基于实验室的 ELISA/SPR 检测格式验证该设备。里程碑：开发出压电 BNS 测定，能够在 <15 分钟反应时间内检测 gp120 和 p24，检测限 <100 pg/ml，同时使用不超过 10 -100 <l 的液体样品。具体目标 2：设计和开发与 HIV/AIDS 相关的多种生物标志物的 BNS 检测 一旦在具体目标 1 中建立了基本 BNS 设备格式的设计和 HIV 检测方法，我们将开发针对传感其他生物标志物进行优化的个体生物芯片。对 HIV 感染患者进行常规评估：包括 CD4、胰岛素、C 肽、IL-6 和 HbA1C，并将其应用于血液和血清分析。因此，这种 BNS 检测将提供 HIV/AIDS 患者的具体健康状况。 BNS 优化将包括确定每个生物标志物的单独反应条件以及相关操作条件，例如：电极尺寸、堆积密度、最佳尺寸、相互作用的声学模式、用于监测多个传感器和信号处理的软件和硬件开发。里程碑：开发 BNS 系统，以皮克灵敏度同时检测生物标记物 gp120、p24、CD4、胰岛素、C 肽、IL-6 和 HbA1C，预计检测成本为 5 - 10 美元/样本 公共卫生相关性：当前针对感染艾滋病毒的老年患者（预计未来几年感染艾滋病毒的老年人数量将增加）以及其他疾病的护理策略面临着与高度复杂的治疗模式相关的挑战，这些治疗模式缺乏协调，因此通常会使护理效果降低。由于多种药物和多种问题，这些患者被迫忍受较低的生活质量，其特点是治疗并发症和医疗效果下降的可能性很高。为了满足这一需求，我们提出了一种干预模型（一种原创的生物纳米传感器检测技术），旨在协调和简化艾滋病毒治疗，特别是针对患有多种疾病的老年人。