Pathology Core

病理学核心

• 批准号: 8195742
• 负责人: JACQUELINE Jones COALSON
• 金额: $ 30.64万
• 依托单位: UNIVERSITY OF TEXAS HLTH SCIENCE CENTER
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-07-01 至 2016-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8195742
• 关键词: Appearance; Asthma; Autopsy; Biopsy; Cells; Clinical; Collaborations; Core Facility; Data; Diagnostic; Documentation; Electrons; Etiology; Evaluation; Glass; Goals; Grant; Histocytochemistry; Image; Image Analysis; Individual; Inflammatory Infiltrate; Length; Lesion; Liquid substance; Literature; Lung; Lymphocyte; Measurable; Measurement; Methods; Microscope; Microscopic; Modeling; Molecular; Mus; Mycoplasma pneumoniae; Organism; Ovum; Pathogenesis; Pathologic; Pathology; Population; Preparation; Procedures; Process; Production; Proteins; Research; Research Personnel; Research Proposals; Resolution; Role; Services; Slide; Specimen; System; Techniques; Testing; Tissues; Toxin; asthmatic patient; cell type; cytokine; diagnosis evaluation; digital; digital imaging; eosinophil; human tissue; immunocytochemistry; light microscopy; mouse model; mutant; pyroglyphid; research study; tissue processing

The Pathology Core will routinely process tissue specimens submitted from the projects and Clinical Core for light microscopy, histochemistry and immunocytochemistry. Due to the high volume of mouse tissues from several bronchial asthma models that require quantitation, an Aperio Image Analysis system, with a stereology module, has been purchased and will be dedicated to the investigative needs of this CRC. It is a digital pathology system which converts entire glass microscope slide specimens into high-resolution, whole slide digital images that can then undergo quantitative analytical techniques. The automated Image quantitative analytical techniques have been shown to shorten research and analysis efforts by 90%, which is critical with the number of slides processed in this core facility (over 3500 during the current CRC grant period). The interpretation of the tissue specimens will be the responsibility of the Core Director and all data will be subsequently shared with the investigators. Projects 1 and 2 and the Clinical Core of this CRC will require substantive Pathology support. The Pathology Core is vital to this CRC because it 1) will process and produce all the routine histopathological specimens for pathologic evaluation and diagnosis, 2) will provide the production of the needed immunocytochemical markers of cell types identified in the mouse models of bronchial asthma that can then be quantified, 3) will produce definitive quantitative data that can be compared and contrasted among different asthma models, 4) will determine the pathological effects of CARDS TX administration in the ova and dust mite asthma models, 5) determine and quantify potential differences in mutant CARDS TX pathologies, and 6) identify M. pneumoniae organisms and CARDS TX in human tissue and fluid specimens. Information gained from the services provided by this core will augment the proposed research plans that will assist the documentation that CARDS TX is an etiologic agent in the pathogenesis of a subset of asthmatic patients.

病理核心将定期处理从项目和临床核心提交的组织标本 光学显微镜，组织化学和免疫细胞化学。由于大量的小鼠组织 几种需要定量的支气管哮喘模型，一个Aperio图像分析系统， 立体学模块已被购买，并将致力于该CRC的调查需求。是一个 数字病理系统将整个玻璃显微镜幻灯片转换为高分辨率，整个 滑动数字图像，然后可以进行定量分析技术。自动图像 已显示定量分析技术将研究和分析工作缩短了90％，这是 对于此核心设施中处理的幻灯片数量至关重要（当前CRC赠款期间的3500多个 时期）。组织标本的解释将是核心主管和所有数据的责任 随后将与调查人员共享。项目1和2以及该CRC的临床核心将 需要实质性病理支持。病理核心对此CRC至关重要，因为它1）将处理和 生产所有常规的组织病理标本以进行病理评估和诊断，2）将提供 在小鼠模型中确定的细胞类型所需的免疫细胞化学标记的产生 然后可以量化的支气管哮喘，3）将产生可以成为的确定定量数据 在不同的哮喘模型之间进行了比较和对比，4）将确定 Cards TX在OVA和尘螨哮喘模型中的给药，5）确定并量化潜力 突变卡TX病理学的差异，以及6）识别肺炎的生物和卡片tx 人体组织和液体标本。从该核心提供的服务中获得的信息将增加 拟议的研究计划将有助于文档，即Cards TX是一个病因学代理 哮喘患者子集的发病机理。