GENETICS AND GENOME BANKING CORE

遗传学和基因组库核心

• 批准号: 8173011
• 负责人: Hans Michael Kubisch
• 金额: $ 6.18万
• 依托单位: TULANE UNIVERSITY OF LOUISIANA
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-05-01 至 2011-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8173011
• 关键词: Alleles; Animals; Back; Blood; Blood Banks; Blood specimen; Cell Line; Chinese People; Computer Retrieval of Information on Scientific Projects Database; DNA; Equilibrium; Equipment and supply inventories; Fibroblasts; Funding; Gene Frequency; Generations; Genetic; Genome; Genotype; Grant; Institution; Methods; Microsatellite Repeats; Output; Parents; Partner in relationship; Research; Research Personnel; Resources; Source; Tandem Repeat Sequences; United States National Institutes of Health; base; father role; genetic resource; male; offspring

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Introduction: The efforts of the Core have focused on banking of genetic resources and on generation of microsatellites to assign paternity and determine allele frequencies. Methods: Blood was collected during routine inventories from which we have amplified small tandem repeats (STRs). The PCR products were sent to UC Davis for size determination. The sequencer output was sent back to us and Core staff makes the final size interpretations of each allele and asigns genotypes. Results/Discussion: During the past year we received 594 STR profiles from animals born from 1983 through 2007 that were based on an older STR panel of 13 loci. From these we have calculated allele frequencies and have determined that the level of heterozygosity has not changed when animals born before and after 2000 are compared. We have established STR profiles using the expanded panel of 34 loci from 230 animals (an additional 190 animals await fragment size determinations at UC Davis), which included 216 Indian and 14 Chinese males. Analysis of these STRs similarly showed a high degree of heterozygosity that does not deviate significantly from what would be expected under equilibrium conditions, such as completely random mating. (observed heterozygosity: 0.75, expected heterozygosity: 0.77). Among the 216 Indian animals were 22 offspring, 109 potential dams and 44 potential sires. We have established paternity for 11 and maternity for 4 of these animals. We do not yet have STR profiles on file for all parents and final parentage determinations will be done when those have arrived. We have collected blood samples from 817 animals, and have obtained DNA extracts from 362 animals. This brings the number of blood-banked animals to 4,356.A total of 100 fibroblast cell lines have been established and cryopreserved, with an additional 50 lines currently in culture.

该副本是利用众多研究子项目之一 由NIH/NCRR资助的中心赠款提供的资源。子弹和 调查员（PI）可能已经从其他NIH来源获得了主要资金， 因此可以在其他清晰的条目中代表。列出的机构是 对于中心，这不一定是调查员的机构。 简介：核心的努力集中在遗传资源和生成微卫星来分配亲子关系并确定等位基因频率上。 方法：在常规库存中收集血液，从中我们放大了小串联重复序列（STR）。 PCR产品已发送到加州大学戴维斯分校的尺寸确定。音序器输出被发送回我们，核心人员对每个等位基因的最终尺寸解释和ASIGN基因型进行了解释。 结果/讨论：在过去的一年中，我们收到了1983年至2007年出生的动物的594个str剖面，这些动物基于13个基因座的较旧的Str面板。通过这些，我们计算出等位基因频率，并确定当比较2000年之前和之后的动物时，杂合性水平没有改变。我们已经使用230只动物的34个基因座（另外190只动物等待碎片尺寸的测定）建立了Str曲线，其中包括216名印度和14名中国男性。 对这些STR的分析类似地显示出高度的杂合性，这与在平衡条件下（例如完全随机交配）下的预期不会显着偏离。 （观察到的杂合性：0.75，预期的杂合性：0.77）。 在216只印度动物中，有22个后代，109个潜在的大坝和44个潜在的sires。我们已经建立了其中4只动物的11个和产妇的亲子关系。我们尚未为所有父母提供文件配置文件，并在到达这些父母的情况下确定最终的父母确定。 我们已经从817只动物那里收集了血液样本，并从362只动物中获得了DNA提取物。这使血液储备的动物数量达到4,356。总共建立了100个成纤维细胞系和冷冻保存，目前正在培养中另外50条线。