GENETICS AND GENOME BANKING CORE

遗传学和基因组库核心

• 批准号: 8358105
• 负责人: Hans Michael Kubisch
• 金额: $ 3.72万
• 依托单位: TULANE UNIVERSITY OF LOUISIANA
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-05-01 至 2012-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8358105
• 关键词: Alleles; Animals; Biopsy; Blood; Blood specimen; Breeding; Cell Line; DNA; DNA Library; Data; Derivation procedure; Development; Equilibrium; Equipment and supply inventories; Fibroblasts; Funding; Gene Frequency; Generations; Genetic; Genome; Grant; Infant; Methods; Microsatellite Repeats; National Center for Research Resources; Outcome; Parents; Partner in relationship; Primates; Principal Investigator; Process; Research; Research Infrastructure; Resources; Sampling; Short Tandem Repeat; Source; Testing; Tissues; United States National Institutes of Health; Universities; age effect; cost; father role; genetic resource; male; working group

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. Introduction: The efforts of the Core have focused on banking of genetic resources and on generation of microsatellites to assign paternity and determine allele frequencies. Methods: Blood and tissue biopsies were collected during routine inventories. Blood was used for DNA extractions and for banking using archival blood cards. Biopsies were processed to establish and cryopreserve fibroblast cell lines. Results/Discussion: During the past year the Core obtained blood samples from 734 animals which were blotted on archival blood cards. The current number of blood samples banked on cards is 5169. The Core also obtained 302 tissue biopsies from which a total of 193 fibroblasts cultured were generated and cryopreserved. Analysis of the outcome of fibroblast derivations revealed a significant age effect with biopsies from older animals yielding fewer cell lines. The number of cell lines banked to date is 329. Short tandem repeats (STRs) were generated from 281 animals and were sent off to the University of Davis (UD) for allele determination. This included samples from 78 SPF4 potential breeding males. To date, results were obtained from UD for 190 animals. Analysis of allele data from these potential breeders revealed that the overall degree of heterozygosity at all loci deviates from what would be expected under equilibrium conditions such as completely random mating (observed: 0.62, expected: 0.72, p=0.006). Paternity and/or maternity were assigned to 36 infants, most of which were part of the SPF9 colony. We do not yet have STR profiles on file for all parents and final parentage determinations will be done when those have arrived. The Core participated in activities of the NPRC Consortium Genome Banking and Genetics Working Groups in the establishment of a DNA bank and the development and testing of SNP panels for paternity an ancestry analysis.

该副本是利用资源的众多研究子项目之一 由NIH/NCRR资助的中心赠款提供。对该子弹的主要支持 而且，副投影的主要研究员可能是其他来源提供的 包括其他NIH来源。 列出的总费用可能 代表subproject使用的中心基础架构的估计量， NCRR赠款不直接向子弹或副本人员提供的直接资金。 简介：核心的努力集中在遗传资源和生成微卫星来分配亲子关系并确定等位基因频率上。 方法：在常规清单期间收集血液和组织活检。血液用于DNA提取，并使用档案血卡进行库存。 进行了活检以建立和冷冻保存成纤维细胞系。 结果/讨论：在过去的一年中，核心从734只动物中获得了血液样本，这些动物被档案血卡印迹。当前在卡片上储存的血液样本数量为5169。核心还获得了302个组织活检，从中产生了193个成纤维细胞培养和冷冻保存。对成纤维细胞衍生的结果的分析表明，来自老年动物的活检产生的年龄效应显着，产生的细胞系较少。迄今为止，存入的细胞系数量为329。从281只动物中产生了短串联重复（STR），并被送往戴维斯大学（UD）进行等位基因确定。这包括来自78个SPF4潜在繁殖雄性的样品。迄今为止，从UD获得了190只动物的UD结果。 对这些潜在繁殖者的等位基因数据的分析表明，所有基因座的杂合性的总体程度远离了在诸如完全随机交配等平衡条件下预期的偏差（观察到：0.62，预期：0.72，p = 0.006）。父亲鉴定和/或产妇分配给36名婴儿，其中大多数是SPF9菌落的一部分。我们尚未为所有父母提供文件配置文件，并在到达这些父母的情况下确定最终的父母确定。核心参与了NPRC财团基因组银行和遗传工作组的活动，以建立DNA库以及SNP小组的开发和测试，以进行亲子关系。