RECOVERY OF EPIDIDYMAL SPERM FROM RHESUS MACAQUES

恒河猴附睾精子的回收

• 批准号: 7958697
• 负责人: Hans Michael Kubisch
• 金额: $ 5.8万
• 依托单位: TULANE UNIVERSITY OF LOUISIANA
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-05-01 至 2010-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7958697
• 关键词: Age; Animals; Autopsy; Computer Retrieval of Information on Scientific Projects Database; Freezing; Funding; Grant; Institution; Macaca mulatta; Methods; Primates; Recovery; Research; Research Personnel; Resources; Sampling; Seminal fluid; Source; Testis; United States National Institutes of Health; assisted reproduction; cell motility; male; sperm cell

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Background: To determine whether epididymal sperm could be retrieved and successfully cryopreserved testes were collected at necropsy from a total of 20 rhesus macaques. Methods: The males ranged in age from 3 to 19 years with an average of 8.5 years. No semen was obtained from three animals, which were younger than 4 years. The remaining 17 samples contained semen with an average sperm cell concentration of 144.0 mio/mL, an average motility of 72.8 % and a mean grade of 2.6. Results/Discussion: After 24 h of culture the overall motility decreased significantly in all samples regardless of treatment to 8.8 % in fresh non-activated and 3.8 % in activated semen at 24 hr. Freezing had a significant effect on semen reducing the mean grade to 1.6 and the immediate post-thaw motility to 42.4 % in non-activated samples. Activation further reduced semen motility after 4 hr in fresh semen (72.8 vs 50.4%), but increased it in semen that had been frozen (14.0 vs 23.2%). The age of the male did not influence semen concentration, grade or initial motility, but proved to be a significant factor in determining motility of frozen-thawed activated semen with lower motility found in samples from older males. Overall the study demonstrates that motile semen can be obtained from post-mortem males, although subsequent studies will have to determine whether the quality is sufficient to facilitate its use in assisted reproduction.

该副本是利用众多研究子项目之一 由NIH/NCRR资助的中心赠款提供的资源。子弹和 调查员（PI）可能已经从其他NIH来源获得了主要资金， 因此可以在其他清晰的条目中代表。列出的机构是 对于中心，这不一定是调查员的机构。 背景：确定是否可以检索附子精子，并从总共20个恒河猕猴中收集在尸检时成功冷冻保存的睾丸。方法：男性年龄从3至19岁不等，平均年龄为8.5岁。 从三只动物那里获得了精液，该动物年轻以下4岁。其余的17个样品包含精液，平均精子细胞浓度为144.0 miO/ml，平均运动性为72.8％，平均等级为2.6。结果/讨论：在培养24小时后，所有样品中的总体运动能力在24小时时在新鲜的非激活中的治疗方法显着降低至8.8％，活化精液中的总体运动率显着降低。冻结对精液将平均等级降低到1.6，而在非激活样本中的立即释放后运动性则具有显着影响。 在新鲜精液中4小时后，激活进一步降低了精液运动性（72.8 vs 50.4％），但在被冷冻的精液中增加了（14.0 vs 23.2％）。男性的年龄不影响精液浓度，等级或初始运动，但事实证明是确定冷冻透射活化精液的运动性的重要因素，其在老年男性的样本中发现了较低的运动性。总体而言，研究表明，可以从验尸后男性获得运动精液，尽管随后的研究必须确定质量是否足以促进其在辅助生殖中的使用。