PERMEABILITY OF MACAQUE SPERM AT SUBZERO TEMP IN PRESENCE OF EXTACELLULAR ICE

在细胞外冰存在的情况下，猕猴精子在零下温度下的渗透性

• 批准号: 7958672
• 负责人: Hans Michael Kubisch
• 金额: $ 5.8万
• 依托单位: TULANE UNIVERSITY OF LOUISIANA
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-05-01 至 2010-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7958672
• 关键词: Area; Cell Membrane Permeability; Cell Volumes; Computer Retrieval of Information on Scientific Projects Database; Cryoprotective Agents; Data; Equation; Freezing; Funding; Glycerol; Grant; Ice; Institution; Isotonic Exercise; Length; Macaca; Macaca mulatta; Methods; Modeling; Mole the mammal; Permeability; Primates; Publishing; Radial; Reporting; Research; Research Personnel; Resources; Source; Surface; Suspension substance; Suspensions; Translating; United States National Institutes of Health; Water; response; sperm cell

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Background: In the present study, we report the effects of cooling ejaculated and epididymal rhesus monkey (Macaca mulatta) sperm with and without the presence of a cryoprotective agent, glycerol. Methods: Water transport data during freezing of ejaculated and epididymal sperm cell suspensions were obtained at a cooling rate of 20 ¿C/min in the absence of any cryoprotective agents and in the presence of 0.7 M of glycerol, as well. Using previously published values, the macaque sperm cell was modeled as a cylinder of length 73.83 ¿m with a radius of 0.40 ¿m and an osmotically inactive cell volume, Vb, of 0.772Vo, where Vo is the isotonic cell volume. This translated to a surface area, SA to initial water volume, WV ratio of ~ 22 ¿m-1. Results/Discussion: By fitting a model of water transport to the experimentally determined volumetric shrinkage data, the best-fit membrane permeability parameters (reference membrane permeability to water at 0 ¿C, Lpg or Lpg[cpa] and the activation energy, ELp or ELp[cpa]) were found to range from: Lpg or Lpg[cpa] = 0.00200.0029 ¿m/min-atm; ELp or ELp[cpa]) = 10.618.3 kcal/mole. By incorporating these membrane permeability parameters in a recently developed equation (optimal cooling rate, Bopt ¿ 1009:5 x exp(-0.0546 x ELp) x (Lpg) x (SA/WV); where the units of Bopt are ¿C/min, ELp or ELp[cpa] are kcal/mole, Lpg or Lpg[cpa] are ¿m/min-atm and SA/WV are ¿m-1), we determined the optimal rates of freezing macaque sperm to be ~ 23¿C/min (ejaculated sperm in the absence of CPAs), ~29 ¿C/min (ejaculated sperm in the presence of glycerol), ~24 ¿C/min (epididymal sperm in the absence of CPAs) and ~ 24 ¿C/min (epididymal sperm in the presence of glycerol). In conclusion, the subzero water transport response and consequently the subzero water transport parameters are not significantly different between the ejaculated and epididymal spermatozoa under corresponding cooling conditions.

该副本是使用众多研究子项目之一 由NIH/NCRR资助的中心赠款提供的资源。子弹和 调查员（PI）可能已经从其他NIH来源获得了主要资金， 因此可以在其他清晰的条目中代表。列出的机构是 对于中心，这是调查员的机构。 背景：在演讲研究中，我们报告了冷却射精和附子恒河猴（Macaca Mulatta）精子的影响，有无冰冷的保护剂甘油。方法：在没有任何冷冻保护剂的情况下，在射精和附睾精子细胞悬浮液的冷冻过程中以20℃和0.7 m的甘油也以20 c/min的形式获得了水传输数据。使用先前发表的值，将猕猴的精子模拟为长度为73.83€m的圆柱体，半径为0.40»m，渗透性无活性的细胞体积Vb为0.772VO，其中vo是等渗细胞体积。这转化为表面积，SA到初始水量，WV比为〜22€M-1。结果/讨论：通过将水传输模型拟合到实验确定的体积收缩数据，最佳拟合膜渗透性参数（参考膜到0？c，lpg或lpg [cpa]时对水的渗透率[CPA]和激活能量，ELP或ELP [CPA]均来自：lpg或lpg [CPA]。 m/min-atm; ELP或ELP [CPA]）= 10.6 18.3 kcal/mole。通过在最近开发的方程式中转换这些膜渗透性参数（最佳冷却速率，BOPT€1009：5 x exp（-0.0546 x ELP）x（lpg）x（sa/wv）x（sa/wv）; bopt单位的bopt单位是€c/min，ELP或ELP [CPA]是kcal/min/lpg，是min/min lpg。 and SA/WV are ¿m-1), we determined the optimal rates of Freezing macaque sperm to be ~ 23¿ C/min (ejaculated sperm in the absence of CPAs), ~29 ¿ C/min (ejaculated sperm in the presence of glycerol), ~24 ¿ C/min (epidymal sperm in the absence of CPAs) and ~ 24 ¿ C/min (epiditical sperm in the presence of甘油）。总之，在相应的冷却条件下，射精和附子精子的射精和附子精子之间的亚零输水反应以及亚零输水参数没有显着差异。